A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Allele Dmel\GlΔ22

General Information
SymbolDmel\GlΔ22SpeciesD. melanogaster
NameFlyBase IDFBal0217579
Feature typealleleAssociated geneDmel\Gl
Allele class
MutagenP-element activity
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Description
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FB2013_03
FB2013_02
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hide Nature of the Allele
Allele class
Mutagen
P-element activity
(Siller et al., 2005)
Mutations Mapped to the Genome
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
GlKG07739
(Siller et al., 2005)
Nature of the lesion
Statement
Reference
Imprecise excision of the insertion in GlKG07739, resulting in a deletion of approximately 3.2kb that part of the Gl transcription unit (including the presumptive transcription start site) and part of the CG8833 transcription unit.
(Siller et al., 2005)
Caused by aberration
Df(3L)GlΔ22
(Siller et al., 2005)
Cytology
hide Phenotypic Data
hide Phenotypic Class
lethal | first instar larval stage (with Gll1)
(Lloyd et al., 2012)
lethal | pupal stage (with GlG38S)
(Lloyd et al., 2012)
mitotic cell cycle defective
(Siller et al., 2005)
neuroanatomy defective | third instar larval stage (with GlG38S)
(Lloyd et al., 2012)
neurophysiology defective | third instar larval stage (with GlG38S)
(Lloyd et al., 2012)
hide Phenotype Manifest In
NMJ bouton | third instar larval stage (with GlG38S)
(Lloyd et al., 2012)
spindle
(Siller et al., 2005)
spindle (with Gll1)
(Siller et al., 2005)
spindle pole centrosome
(Siller et al., 2005)
spindle pole centrosome (with Gll1)
(Siller et al., 2005)
hide Detailed Description
Statement
Reference
Gl[G38S]/Gl[Δ22] larvae show normal locomotion. Gl[G38S]/Gl[Δ22] third instar larvae have a normal number of synaptic boutons per neuromuscular junction (NMJ) in proximal abdominal segments (A2 and A3) but show a small but significant increase in the number of synaptic boutons per NMJ in distal segments (A5 and A6). The terminal boutons (the distal-most synaptic boutons) are swollen at the mutant NMJ and show an approximately 2-fold increase in bouton volume in distal segments. Gl[G38S]/Gl[Δ22] larvae show a significant reduction in the amplitude of the evoked junctional potential (EJP) at the NMJ compared to controls.
(Lloyd et al., 2012)
GlΔ22/Gll1 and homozygous neuroblasts show centrosome and spindle formation defects. Defects include metaphase neuroblasts with curved spindles, unfocused spindle poles, spindles with one or two unattached centrosomes, spindles in which both centrosomes are attached to the same half-spindle, or occasionally neuroblasts with three or four centrosomes forming bipolar or multipolar spindles. The mitotic index and metaphase:anaphase ratio are increased in homozygous neuroblasts compared to wild type.
(Siller et al., 2005)
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Linkouts
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hideEnhanced by
Statement
Reference
GlG38S/GlΔ22 has phenotype, enhanceable by Khc[+]/Khc8
(Lloyd et al., 2012)
hide Additional Comments
hide Genetic Interactions
Statement
Reference
Gl[G38S]/Gl[Δ22] ; Khc[8]/+ animals rarely survive to pupal stages.
(Lloyd et al., 2012)
hide Xenogenetic Interactions
Statement
Reference
hide Complementation & Rescue Data
Rescued by
GlΔ22/Gll1 is rescued by GlCH322-82J07
(Lloyd et al., 2012)
Partially rescued by
GlΔ22/Gll1 is partially rescued by GlScer\UAS.cLa/Scer\GAL4T80
(Lloyd et al., 2012)
Comments
Expression of Gl[Scer\UAS.cLa] under the control of Scer\GAL4[T80] rescues the lethality of Gl[l1]/Gl[Δ22] animals, although the rescued animals are sterile. Expression of Gl[CH322-82J07] fully rescues Gl[l1]/Gl[Δ22] animals (the rescued animals are viable and fertile).
(Lloyd et al., 2012)
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Discoverer
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Other Crossreferences
Linkouts
hide Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym
GlΔ22
(Siller et al., 2005, Lloyd et al., 2012)
Name Synonym
Secondary FlyBase IDs
hide References ( 2 )
Research paper
Lloyd et al., 2012, Neuron 74(2): 344--360
The p150(Glued) CAP-Gly Domain Regulates Initiation of Retrograde Transport at Synaptic Termini. [FBrf0218160]
Siller et al., 2005, Mol. Biol. Cell 16(11): 5127--5140
Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control. [FBrf0191164]