Open Close
General Information
Symbol
Dmel\PdfrUAS.cMa
Species
D. melanogaster
Name
FlyBase ID
FBal0218036
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Pdfr, UAS-pdfr-16
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The pdfr open reading frame (2010bp), derived from the RH51348 EST sequence, plus an additional 108bp exon at position 424, is cloned into pPUAST.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of PdfrScer\UAS.cMa fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing Ac3Scer\UAS.cDa under the control of Scer\GAL4P2.4.Pdf.

Co-expression of PdfrScer\UAS.cMa fully rescues the response of the M pacemaker neurons in the brain to Pdf to wild-type levels in flies expressing G-sα60AScer\UAS.cCa under the control of Scer\GAL4P2.4.Pdf.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of PdfrScer\UAS.cMa under the combined control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS, but not under the control of either Scer\GAL4Clk.4.5F or Scer\GAL4Pdf.PU, rescues the lower night-onset preferred temperature decrease observed in Pdfr5304 homozygous mutant adults; Pdfr5304 homozygous mutant adults expressing PdfrScer\UAS.cMa under the combined control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS also display significantly higher daytime preferred temperature increase compared to controls.

Expression of PdfrScer\UAS.cMa in DN1[[p]]s under the control of Scer\GAL4Clk.4.5F restores normal light-dependent temperature preference in Pdfr5304 mutants.

The advanced evening peak of locomotor activity which is seen in Pdfr3369 flies under light-dark conditions is rescued by expression of PdfrScer\UAS.cMa under the control of one of Scer\GAL4Pdfr.B, Scer\GAL4cry.PE, Scer\GAL4tim.PE or Scer\GAL4dimm-929, but is not rescued by expression of PdfrScer\UAS.cMa under the control of one of Scer\GAL4P2.4.Pdf, Scer\GAL4cry.PZ or Scer\GAL4Aph-4-c232.

Under constant darkness conditions (measured on days 3-9), expression of PdfrScer\UAS.cMa under the control of Scer\GAL4cry.PZ restores strong rhythmic behavior in Pdfr3369 flies, but the phase is delayed 6-10 hours. In approximately 50% of the rhythmic flies this delay happens instantaneously, whereas in others it takes 3-4 days to shift and then free-run with a period of 23.7 hours.

Expression of pdfrScer\UAS.cMa using Scer\GAL4Clk.8.0 in a pdfr5304 background rescues the defects in morning anticipation and the timing of light-dark entrained evening behaviour.

Expression of pdfrScer\UAS.cMa using Scer\GAL4cry.PE in a pdfr5304 background restores the timing of evening behaviour and also promotes significant restoration of morning behaviour during light-dark entrainment and the first day of constant dark.

Expression of pdfrScer\UAS.cMa only in "evening" cells using Scer\GAL4cry.PE together with Scer\GAL80Pdf.PS in a pdfr5304 background rescues both the timing of evening behaviour and the magnitude of morning anticipation during light-dark entrainment and the first day of constant dark.

Expression of pdfrScer\UAS.cMa only in "morning" cells using Scer\GAL4P2.4.Pdf in a pdfr5304 background does not rescue the timing of either morning or evening behaviours during light-dark entrainment and the first day of constant dark.

Expression of pdfrScer\UAS.cMa using Scer\GAL4Clk.8.0 in a pdfr5304 background promotes significant rescue of rhythmicity and period length in constant dark.

Expression of pdfrScer\UAS.cMa using Scer\GAL4cry.PE in a pdfr5304 background rescues period length and partially restores rhythmicity in constant dark.

Expression of pdfrScer\UAS.cMa only in "evening" cells using Scer\GAL4cry.PE together with Scer\GAL80Pdf.PS in a pdfr5304 background does not rescue rhythmic power though does rescue period length.

Expression of pdfrScer\UAS.cMa only in "morning" cells using Scer\GAL4P2.4.Pdf in a pdfr5304 background has no significant effect on either free-running rhythmicity or period length in constant darkness.

Expression of pdfrScer\UAS.cMa using Scer\GAL4elav.PLu in a pdfr5304 background rescues all circadian rhythm phenotypes in both light-dark and constant dark conditions. Co-expression of Scer\GAL80Pdf.PS results in a substantial reduction in rescue of rhythmic power in constant dark, though doesn't affect the rescue of period length in constant dark.

Expression of pdfrScer\UAS.cMa using Scer\GAL4Pdfr-EY11181-GAL4 in a pdfr5304/pdfrEY11181-GAL4 background strongly rescues the reduced rhythmicity phenotype seen in constant dark conditions.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PdfrScer\UAS.cMa
PdfrUAS.cMa
pdfrScer\UAS.cMa
Name Synonyms
Secondary FlyBase IDs
    References (10)