Pdfr5304 does not suppress the increase in the levels of (z)-7-Tricosene and (z)-7-Pentacosene compared to wild type seen in males expressing PdfScer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1 under the control of Scer\GAL4desat1.PB (measured on the sixth day of constant darkness).
Expression of PdfrScer\UAS.cMa under the combined control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS, but not under the control of either Scer\GAL4Clk.4.5F or Scer\GAL4Pdf.PU, rescues the lower night-onset preferred temperature decrease observed in Pdfr5304 homozygous mutant adults; expression under the combined control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS also significantly exacerbates the day-time preferred temperature increase observed in Pdfr5304 homozygous mutant adults.
PdfrT:Hsap\MYC rescues both the advanced evening peak of locomotor activity which is seen in Pdfr5304 flies under light-dark conditions and the arrhythmicity of Pdfr5304 flies under constant darkness conditions.
The evening phase-advanced anticipation seen in pdfr5304 mutants is not rescued by expression of pdfrScer\UAS.cZa under the control of Scer\GAL4Clk.4.5F or Scer\GAL4Clk.4.1M. In contrast, morning anticipation is resuced by both Scer\GAL4Clk.4.1M and Scer\GAL4Clk.4.5F expression of pdfrScer\UAS.cZa.
Behaviour rhythms of pdfr5304 mutants are rescued by Scer\GAL4Clk.4.1M-driven expression of pdfrScer\UAS.cZa. There is significant improvements of rhythm amplitude with Scer\GAL4Clk.4.1M-driven expression of pdfrScer\UAS.cZa but not with Scer\GAL4Clk.4.5F-driven expression.
Expression of pdfrScer\UAS.cMa using Scer\GAL4cry.PE in a pdfr5304 background restores the timing of evening behaviour and also promotes significant restoration of morning behaviour during light-dark entrainment and the first day of constant dark.
Expression of pdfrScer\UAS.cMa only in "evening" cells using Scer\GAL4cry.PE together with Scer\GAL80Pdf.PS in a pdfr5304 background rescues both the timing of evening behaviour and the magnitude of morning anticipation during light-dark entrainment and the first day of constant dark.
Expression of pdfrScer\UAS.cMa only in "morning" cells using Scer\GAL4P2.4.Pdf in a pdfr5304 background does not rescue the timing of either morning or evening behaviours during light-dark entrainment and the first day of constant dark.
Expression of pdfrScer\UAS.cMa only in "morning" cells using Scer\GAL4P2.4.Pdf in a pdfr5304 background has no significant effect on either free-running rhythmicity or period length in constant darkness.
Expression of pdfrScer\UAS.cMa using Scer\GAL4elav.PLu in a pdfr5304 background rescues all circadian rhythm phenotypes in both light-dark and constant dark conditions. Co-expression of Scer\GAL80Pdf.PS results in a substantial reduction in rescue of rhythmic power in constant dark, though doesn't affect the rescue of period length in constant dark.