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General Information
Symbol
Dmel\SuURUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Zhimulev
FlyBase ID
FBal0218109
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-SuUR, UAS-SuUR+
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of the full ORF and 3'UTR of the SuUR cDNA is governed by UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4Sgs3.PD-mediated expression of SuURScer\UAS.cZa leads to formation of large chromosomal swellings in the intercalary heterochromatin regions, such as the 75C1-2 region.

Polytene chromosomes in larvae expressing SuURScer\UAS.cZa under the control of Scer\GAL4Sgs3.PD show swelling in the regions 24D, 25A, 56F, 67A, 67D and 75C.

Expression of SuURScer\UAS.cZa under the control of Scer\GAL4AB1 results in a dramatic reduction in the size of larval salivary gland nuclei compared to controls.

Expression of SuURScer\UAS.cZa under the control of Scer\GAL4AB1 results in the formation of miniature salivary glands. The nuclei of the salivary glands cells are also significantly smaller than normal. Weak points in the polytene chromosomes are still seen in these animals.

Expression of SuURScer\UAS.cZa under the control of Scer\GAL4Sgs3.PD results in the appearance of swellings in intercalary heterochromatin regions of the salivary gland polytene chromosomes.

The polytene chromosomes of the salivary glands of SuURScer\UAS.cZa; Scer\GAL4Sgs3.PD larvae grown at 25'C develop a characteristic pattern of swellings beginning at mid-third instar. By late (wandering) third instar stage, some of these swellings become very large. The size and number of swellings peaks in mid prepupal stages (4-8 hours APF). This phenotype is enhanced at 29'C, suppressed at 18'C and is reversible by switching larvae displaying these swellings at 29'C to 18'C. The number (about 190) and cytological pattern of swellings is highly reproducible, but is completely different from the pattern of chromosome puffs seen in wild-type: swellings arise in chromosome regions where puffs never appear ; the sites of their formation are normally tightly condensed, solid bands and some, such as the region of the Antp and bithorax complexes, are well known examples of silenced regions in intercalary heterochromatin. Analysis of all 190 polytene chromosome bands where swellings appear shows that they represent the regions of intercalary heterochromatin characterized by late replication or underreplication. 3Huridine labelling shows that, unlike chromosome puffs, these swellings are not sites of transcription.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

No chromosomal swellings occur in the 75C1-2 region in Df(3L)ED225 chromosomes when SuURScer\UAS.cZa is expressed via Scer\GAL4Sgs3.PD.

Chromosomal swellings of nearly the same size as controls occur in the 75C1-2 region in Df(3L)ED224 or Df(3L)H99 chromosomes when SuURScer\UAS.cZa is expressed via Scer\GAL4Sgs3.PD.

Chromosomal swellings that are smaller than controls occur in the 75C1-2 region in Df(3L)NA34 chromosomes when SuURScer\UAS.cZa is expressed via Scer\GAL4Sgs3.PD.

Chromosomal swellings that are the same size as controls occur in the natural and duplicated parts of the 75C1-2 region of Dp(3L)NA18 chromosomes when SuURScer\UAS.cZa is expressed via Scer\GAL4Sgs3.PD.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
SuURScer\UAS.cZa
SuURUAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct of Zhimulev
Secondary FlyBase IDs
    References (8)