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General Information
Symbol
Dmel\DgO86
Species
D. melanogaster
Name
FlyBase ID
FBal0218114
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C16089139T

Reported nucleotide change:

C880T

Amino acid change:

R87term | Dg-PA; R87term | Dg-PB; R87term | Dg-PC; R87term | Dg-PD

Reported amino acid change:

R87term

Comment:

Reported coordinates appear to be relative to GB:GH09323.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C880T.

Amino acid replacement: R87term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

DgO55/DgO86 show no significant changes in systolic diameter, diastolic diameter or fractional shortening compared to wild type.

Induction of DgO86 mutant clones causes abnormal clustering between clonal and non-clonal cells, resulting in altered 'cobblestone-like' brain organisation.

DgO86 mutant larval brains contain fewer actively dividing S-phase neuronal stem cells per lobe compare to controls. There are also fewer M-phase neuroblasts per lobe than in controls and this is also seen in DgO86 mutant clones.

DgO86 follicle cell clones show normal polarity under both well-fed and starvation conditions. Polarity is also maintained after feeding flies drugs that reduce cellular ATP levels and activate AMPK, though many egg chambers degenerate at stage 7/8.

Homozygous DgO86 mutant flies exhibit indirect flight muscle degeneration.

DgO86/+ mutant flies exhibit temperature-induced mobility defects.

DgO86/DgO55 mutant flies exhibit temperature-induced mobility defects.

DgO86/Df(3R)Exel6184 mutant flies exhibit temperature-induced mobility defects.

Increased indirect flight muscle degeneration is seen in DgO86/DgO55 mutant flies at 25[o]C and, as in wild type, the phenotype is increased ~2 fold by an increase in temperature to 33[o]C. On paraquat-containing food the ratio or degree of muscle degeneration is not increased in DgO86/DgO55 mutant flies in comparison to control. Unlike in wild type, animals exposure of DgO86/DgO55 mutants to lower temperature (18[o]C) causes the appearance of severe progressive degeneration, followed by focal muscle loss. At 25[o]C the phenotype does not become significantly more severe with age. Sugar-free food conditions promote severe muscle loss. Metabolic rate on sugar-free food is reduced compared to wild type controls.

DgO86 homozygous as well as DgO86/DgO55 transheterozygous third instar larvae frequently show lamina plexus defects in the brain. Adult DgO86/DgO55 transheterozygotes also display significantly reduced rhabdomere length compared to wild-type.

DgO86 homozygous somatic clones in the pupal retina have shorter ommatidia and display nuclei layering defects as well as aberrant actin distribution compared to wild-type cells.

More than 80% of DgO86 flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, while most of the remaining mutant flies have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5.

Approximately 60% of DgO86/Dg248 flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, while most of the remaining mutant flies have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5.

Approximately 20% of DgO86/Dg323 flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, approximately 60% have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5 and the remainder if the flies have a complete crossvein as in wild type.

The Dg[-] progeny of DgO38/DgO86 females mated to DgO43/+ males are viable when grown without competition from heterozygous larvae.

The Dg[-] progeny of DgO43/DgO86 females mated to DgO55/+ males are viable when grown without competition from heterozygous larvae.

The Dg[-] progeny of DgO55/DgO86 females mated to DgO38/+ males are viable when grown without competition from heterozygous larvae.

DgO86/DgO38, DgO86/DgO43 and DgO86/DgO55 animals show reduced viability under normal culture conditions.

73% of eggs laid by DgO38/DgO86 females mated to wild-type males hatch.

67% of eggs laid by DgO43/DgO86 females mated to wild-type males hatch.

66% of eggs laid by DgO55/DgO86 females mated to wild-type males hatch.

85% of eggs laid by DgO38/DgO86 females mated to DgO43/+ males hatch.

79% of eggs laid by DgO43/DgO86 females mated to DgO55/+ males hatch.

82% of eggs laid by DgO55/DgO86 females mated to DgO38/+ males hatch.

89% of eggs laid by Dg248/DgO86 females mated to wild-type males hatch.

83% of eggs laid by Dg323/DgO86 females mated to wild-type males hatch.

Females carrying homozygous germline clones produce eggs that are normal in shape.

Eggs laid by homozygous females are shorter and rounder than wild type.

DgO43/DgO86 follicle cells show a defect in the organisation of the actin stress fibres; the fibres are still properly aligned in mutant follicle cells, but they do not align along the dorsal-ventral axis, and they sometimes show a mixed orientation within a single cell.

The stress fibres are randomly oriented in homozygous follicle cell clones. This phenotype is cell autonomous.

Under normal food conditions, DgO86 mutant follicle cell clones have normal apical-basal polarity.

Homozygotes show a posterior crossvein phenotype with 100% penetrance. More than 80% of wings show a "detached" phenotype (the crossvein is detached from both L4 and L5). A fraction of wings with a "gapped" phenotype (the crossvein is detached from either vein L4 or L5, but not both) or a "point" phenotype (the crossvein is reduced to a point) are seen.

DgO86/Df(2R)ED2457 animals show 77% viability compared to controls.

The number of hemocytes in the posterior crossvein region in homozygous pupal wings is either greatly reduced compared to wild type or they are completely absent.

DgO86/DgO55 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

DgO86/DgO43 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

DgO86 has posterior crossvein phenotype, suppressible by Dl9P/Dl[+]

Other
Statement
Reference

Dg[+]/DgO86, PgkKG07478 has indirect flight muscle | nutrition conditional phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Dg Dys double heterozygous flies (DgO86/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

chifBG02820a DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

chifEY05746 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

Lis-1k11702 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

Lis-1k13209 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

Nrkk14301 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

FhosEY09842 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

ItgbnBG01037 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

CG7845EMS-Mod4 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

mblE27 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

Rack1EE DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

Rack11.8 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

dyscc03838 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

uif2B7 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

uif1 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

PgkKG07478 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration when raised on normal food, but this degeneration is not seen when flies are raised on sugar-free food. In contrast to either heterozygote alone, temperature -sensitive mobility defects are observed in the double heterozygotes.

vimar09 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

Fkbp1400734 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

SP2353MB00605 DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

captE636 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

captE593 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Lis-1G10.14 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

nAChR╬▒6EY13897 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

nAChR╬▒6KG05852 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Camn339 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Rack1EY00128 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

gcmKG01117 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

vimark16722 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

POSHk15815 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

delKG10262 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

grhIM DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

robo12 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Combination of DgO86 in heterozygous state with a single copy of any of the following: Df(3R)Exel6184, dyscc05107, Camn339 results in significantly increased frequency of lamina plexus defects in third instar larvae. The frequency is not significantly affected by combination with: captE636, vimarEY09646, robo12, SP2353MB00605, mblE27, grhIM or Nrkk14301 alleles and is decreased by combination with chifBG02820a - all in heterozygous state.

Combination of DgO86 in heterozygous state with a single copy of any of the following :Df(3R)Exel6184, Camn339, captE636 or mblE27 results in a significantly reduced rhabdomere length in the double heterozygous flies whereas combination with one copy of Nrkk14301 has no significant effect.

The increased frequency of lamina plexus defects characteristic for Lis-1k13209 heterozygote third instar larvae is not strongly affected by combination with a single copy of either Df(3R)Exel6184 or DgO86, the rhabdomere length in the adult eye of the double heterozygotes is not significantly different from the controls.

The "detached" posterior crossvein phenotype seen in DgO86 homozygotes is suppressed by Dl9P/+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (10)