Allele Hsap\KCNJ2^{AAE.Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Hsap\KCNJ2^{AAE.Scer\UAS.T:Avic\GFP-EGFP}	Species	H. sapiens
Name		FlyBase ID	FBal0218118
Feature type	allele	Associated gene	Hsap\KCNJ2

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Paradis et al., 2001) in vitro construct - coding region fusion (Paradis et al., 2001) in vitro construct - regulatory fusion (Paradis et al., 2001) in vitro construct - site directed mutagenesis (Paradis et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\KCNJ2 in which the putative PDZ binding consensus site (SEI) has been replaced with AAE. The protein is also tagged with T:Avic\GFP^EGFP. (Paradis et al., 2001) Amino acid replacement: E?A. (Paradis et al., 2001) Amino acid replacement: S?A. (Paradis et al., 2001) Amino acid replacement: I?E. (Paradis et al., 2001)
Carried in construct	P{UAS-Kir2.1.AAE} (Paradis et al., 2001)
Tagged with	T:Avic\GFP^EGFP (Paradis et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	embryonic/larval muscle system, with Scer\GAL4^Mhc.PW (Paradis et al., 2001)
Detailed Description
	Statement Reference Expression of Hsap\KCNJ2^{AAE.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW alters the passive membrane properties of muscles. The resting membrane potential is hyperpolarised, the input resistance is decreased and the muscle membrane time constant is faster compared to controls. The average amplitude of spontaneous miniature-release events (mEPSPs) of muscles expressing Hsap\KCNJ2^{AAE.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW is significantly smaller than that of controls. Evoked synaptic depolarisations (EPSPs) depolarise the muscle to the same absolute membrane potential as in the wild type. (Paradis et al., 2001)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\KCNJ2^{AAE.Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Paradis et al., 2001, Neuron 30(3): 737--749 Homeostatic control of presynaptic release is triggered by postsynaptic membrane depolarization. [FBrf0137070]

Allele Hsap\KCNJ2AAE.Scer\UAS.T:Avic\GFP-EGFP

Allele Hsap\KCNJ2^{AAE.Scer\UAS.T:Avic\GFP-EGFP}