Open Close
General Information
Symbol
Scer\GAL4GH146
Species
S. cerevisiae
Name
FlyBase ID
FBal0218122
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GH146-GAL4, GH146, GAL4-GH146
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
transposable element insertion site
(Donnelly et al., 2020, Grabe et al., 2020, He et al., 2020, Hing et al., 2020, Lee et al., 2020, Bhukel et al., 2019, Bielopolski et al., 2019, Brown et al., 2019, Jantrapirom et al., 2019, Nojima et al., 2019, Phan et al., 2019, Wu et al., 2019, Zhao et al., 2019, Zhou et al., 2019, Astigarraga et al., 2018, Bakthavachalu et al., 2018, Bronk et al., 2018, Davie et al., 2018, Flybrain Neuron Database et al., 2018.6.28, Fulterer et al., 2018, Gouzi et al., 2018, Hussain et al., 2018, Jeanne et al., 2018, Jing et al., 2018, Louis et al., 2018, Lüdke et al., 2018, Mayseless et al., 2018, Utashiro et al., 2018, Zhao et al., 2018, Bienkowski et al., 2017, Boppana et al., 2017, Franco et al., 2017, Kremer et al., 2017, Li et al., 2017, Martelli et al., 2017, Sethi and Wang, 2017, Tobin et al., 2017, Wu et al., 2017, Wu et al., 2017, Carreira-Rosario et al., 2016, Cervantes-Sandoval et al., 2016, Drago and Davis, 2016, Gai et al., 2016, Grabe et al., 2016, Guven-Ozkan et al., 2016, Miyake et al., 2016, Ng et al., 2016, Rybak et al., 2016, Scholz-Kornehl and Schwärzel, 2016, Vogt et al., 2016, Fosque et al., 2015, Gao et al., 2015, Hartenstein et al., 2015, Haynes et al., 2015, Macpherson et al., 2015, Mullin et al., 2015, Okumura et al., 2015, Pech et al., 2015, Sakuma et al., 2015, Andlauer et al., 2014, Fişek and Wilson, 2014, Kohl et al., 2014, Kohl et al., 2014, Lin et al., 2014, Luhur et al., 2014, Mishra-Gorur et al., 2014, Sakuma et al., 2014, Schubert et al., 2014, Sudhakaran et al., 2014, Wu et al., 2014, Bader et al., 2013, Cao et al., 2013, Chen et al., 2013, Das et al., 2013, Fujita et al., 2013, Levy and Larsen, 2013, Liang et al., 2013, Li et al., 2013, Li et al., 2013, Lin et al., 2013, Lin et al., 2013, Melnattur et al., 2013, Pech et al., 2013, Sekine et al., 2013, Tamura et al., 2013, Wu et al., 2013, Acebes et al., 2012, Akerboom et al., 2012, Berdnik et al., 2012, Butcher et al., 2012, Chen et al., 2012, Hong et al., 2012, Houot et al., 2012, Kao et al., 2012, Kuo et al., 2012, Lin et al., 2012, Masuyama et al., 2012, Moraru et al., 2012, Naganos et al., 2012, Sudhakaran et al., 2012, Tanaka et al., 2012, Volders et al., 2012, Weng et al., 2012, Awasaki et al., 2011, Chen et al., 2011, Christiansen et al., 2011, Das et al., 2011, Hampel et al., 2011, Li et al., 2011, López-Arias et al., 2011, McCann et al., 2011, Michels et al., 2011, Mochizuki et al., 2011, Ping and Tsunoda, 2011, Pitman et al., 2011, Sweeney et al., 2011, Thum et al., 2011, Wang et al., 2011, Wu et al., 2011, Chou et al., 2010, Das et al., 2010, de Haro et al., 2010, Diao et al., 2010, Hekmat-Scafe et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Potter and Luo, 2010, Ruta et al., 2010, Shuai et al., 2010, Tamura et al., 2010, Tea et al., 2010, Yu et al., 2010, Yu et al., 2010, Avery et al., 2009, Blum et al., 2009, Gordon and Scott, 2009, Gu et al., 2009, Hong et al., 2009, Hong et al., 2009, Ignell et al., 2009, Leiss et al., 2009, Liu and Davis, 2009, Masuda-Nakagawa et al., 2009, Sakurai et al., 2009, Tian et al., 2009, Berdnik et al., 2008, Berdnik et al., 2008, Bolduc et al., 2008, Chen et al., 2008, Das et al., 2008, Hong et al., 2008, Lai et al., 2008, Lichtneckert et al., 2008, Root et al., 2008, Schuldiner et al., 2008, Tanaka et al., 2008, Chihara et al., 2007, Keleman et al., 2007, Komiyama and Luo, 2007, Komiyama et al., 2007, Lin et al., 2007, Rolls et al., 2007, Roy et al., 2007, Schwaerzel et al., 2007, Shang et al., 2007, Shi et al., 2007, Spletter et al., 2007, Yao et al., 2007, Zhang et al., 2007, Ashraf et al., 2006, Berdnik et al., 2006, Bhandari et al., 2006, Lai and Lee, 2006, Zhu et al., 2006, Zhu et al., 2006, Bettencourt da Cruz et al., 2005, Marin et al., 2005, Masuda-Nakagawa et al., 2005, Melcher and Pankratz, 2005, Ramaekers et al., 2005, Riemensperger et al., 2005, Stockinger et al., 2005, Wilson and Laurent, 2005, Hummel and Zipursky, 2004, Jefferis et al., 2004, Jhaveri et al., 2004, Suh et al., 2004, Wang et al., 2004, Wilson et al., 2004, Yu et al., 2004, Zhu and Luo, 2004, Ang et al., 2003, Hummel et al., 2003, Komiyama et al., 2003, Wang et al., 2003, Fiala et al., 2002, Jhaveri and Rodrigues, 2002, Ng et al., 2002, Python and Stocker, 2002, Python and Stocker, 2002, Schwaerzel et al., 2002, Wong et al., 2002, Heimbeck et al., 2001, Jefferis et al., 2001, Stocker et al., 1997)
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion into the 5' upstream region of the CG17390 annotation (part of the Oaz gene).

Insertion components
P{GawB}GH146
Product class / Tool use(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in projection neurons, particularly antennal lobe projection neurons.

Scer\GAL4GH146 is expressed in several cells of the adult brain, including a subset of antennal lobe projection neurons and in the mushroom body anterior paired lateral neurons.

Scer\GAL4GH146 labels a subset of projection neurons and the mushroom body anterior paired lateral cell. Co-expression of Scer\GAL4GH146 with a presynaptic marker preferentially labels the presynaptic terminals of the mushroom body anterior paired medial cell throughout the mushroom body with stronger expression in the alpha'/beta' lobes.

Scer\GAL4GH146 labels the antennal lobe projection neurons. Co-expression with a postsynaptic marker reveals that in the mushroom body calyx, these sites are juxtaposed to the presynapses of the Kenyon cells.

Labelling is observed in olfactory projection neurons.

Scer\GAL4GH146 is estimated to be expressed in one fifth of the lPN population.

Scer\GAL4GH146 is expressed in an average of 73, 193 and 49 cells of the adPN, lPN and vPN lineages, respectively.

Scer\GAL4GH146 is expressed in approximately 60% of projection neurons. An estimated 94% of cells expressing Scer\GAL4GH146 are cholinergic.

Although most projection neurons are not GABA-immunopositive, approximately 6 Scer\GAL4GH146 positive neurons, located ventral and slightly lateral to the antennal lobes, are GABA-positive.

Scer\GAL4GH146 is expressed by approximately 90 projection neurons that innervate approximately 40 of the roughly 50 adult antennal lobe glomeruli. Scer\GAL4GH146 is expressed in presumptive larval projection neurons that send neurites that appear to innervate the mushroom body calyx and the larval equivalent of the adult lateral horn.

Scer\GAL4GH146 is estimated to express in about two thirds of the antennal lobe projection neurons.

Use of UAS-GFP with nuclear localization signal allows visualization of cell bodies. In the adult brain, expression observed in clusters of cells (identified as projection neurons) surrounding the antennal lobe in 3 anatomically distinct groups: ~41 cells dorsal-anterior, ~34 cells lateral and ~7 cells ventral. In addition, expression observed in small number of cells that are not projection neurons: 6 cells posterior and lateral to the protocerebrum and 3 cells ventral to the subesophageal ganglion.

Use of UAS-CD8-GFP allows visualization of axons and dendrites. In the adult brain, the 'projection neurons' (relay interneurons) extend dendrites to specific glomeruli in the antennal lobe; the majority send dendrites to only one glomerulus. Projection neurons that innervate the same glomerulus exhibit similar patterns of axon arborization. The majority extend axons through the iACT and synapse both in the calyx of the mushroom body and the lateral protocerebrum; a small number extend axons through the mACT or the oACT, bypass the mushroom body, and connect to the protocerebrum.

The majority of axons from neurons in which Scer\GAL4GH146 expresses project through the inner antenno-cerebral tract and synapse in both the calyx of the mushroom body and the lateral cerebrum. It is expressed in at least 4 neurons that send axons through the middle antenno-cerebral tract and a single neuron projecting through the outer antenno-cerebral tract. These latter two tracts bypass the mushroom body and connect directly with the protocerebrum.

Scer\GAL4GH146 is expressed in some subset of adult antennal lobe projection neurons of the ventral, antero-dorsal and ventral antennal lobe neuroblast lineages. Because of this expression it specifically marks a number of adult antennal lobe glomeruli, the inner and medial antenno-cerebral tracts and parts of the mushroom body calyx and lateral horn.

Scer\GAL4GH146 is expressed in approximately 90 projection neurons.

In adult brain, expressed in lateral protocerebrum (LPR), relay interneurons, inner and median antenno-cerebral tracts (iACT, mACT), and calyx of mushroom body; also in 'descending interneurons' ventral to the LPR, with processes extending into the iACT. In pupal brain, expression first observed 24h APF.

 
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

antennal glomerulus & neuron, with acj61,4.UAS

antennal glomerulus & neuron, with vvlUAS.cCa

axon & adult antennal lobe projection neuron | somatic clone, with lola4.7.UAS, lolae76

axon & adult antennal lobe projection neuron | somatic clone, with lolaL.UAS

axon & adult antennal lobe projection neuron | somatic clone, with lolaL.UAS, lolae76

axon & adult antennal lobe projection neuron | somatic clone | heat sensitive, with lola4.7.UAS

dendrite & adult antennal lobe projection neuron | somatic clone, with lola3.8.UAS, lolae76

dendrite & adult antennal lobe projection neuron | somatic clone, with lola4.7.UAS, lolae76

dendrite & adult antennal lobe projection neuron | somatic clone, with lolaL.UAS

dendrite & adult antennal lobe projection neuron | somatic clone, with lolaL.UAS, lolae76

dendrite & adult antennal lobe projection neuron | somatic clone | heat sensitive, with lola4.7.UAS

dendrite & antennal glomerulus | somatic clone, with Sema1aUAS.cYa

neuron & antennal glomerulus, with Cbeta\DT-AI.UAS

olfactory sensory organ & neuron & dendrite, with acj61,4.UAS

olfactory sensory organ & neuron & dendrite, with vvlUAS.cCa

Detailed Description
Statement
Reference

Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4GH146 cells in adults during testing blocks the increase in aversive olfactory 24 h memory observed when the recall environment better matches the training environment (presence of copper grid), compared to controls.

Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.UAS.Citrine) of Scer\GAL4GH146 neurons in subregions of antennal lobe glomeruli results in activation or inhibition of lateral horn neurons and activation of Kenyon cells.

Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by Scer\GAL4GH146 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4GH146 cells in adult females significantly reduces attraction to vinegar, with no effect on avoidance of E2-hexenal or acetic acid, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

TrpA1UAS.(B).cKa/Scer\GAL4GH146 is a non-suppressor of smell perception defective | heat sensitive phenotype of shakB2

Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference

vvlUAS.cCa, Scer\GAL4GH146, Scer\GAL4GH146 is an enhancer of antennal glomerulus DL1 & neuron | somatic clone phenotype of acj66

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
GAL4GH146
Scer\GAL4GH146
Scer\GAL4H146
Name Synonyms
Secondary FlyBase IDs
  • FBal0061906
  • FBal0197461
References (211)