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General Information
Symbol
Dmel\Cdk8K185
Species
D. melanogaster
Name
FlyBase ID
FBal0218350
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Approximate endpoints of an 882 bp deletion associated with the imprecise excision of P{EP}I-2GE26681.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{EP}I-2GE26681, resulting in retention of the P{EP} extremity in I-2 and deletion of 882 bp proximal to the original P{EP}I-2GE26681 insertion, including the C-terminal one third of Cdk8 protein-coding sequences.

Insertion components
P{?EP}I-2GE26681
Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pupariation is delayed by approximately 2-3 days in homozygous animals. This developmental delay is slightly rescued by feeding with 20-hydroxyecdysone.

96% of homozygous prepupae fail to evert their anterior spiracles, and the prepupae are partially separated from the pupal case.

The level of ecdysteroids is significantly lower than normal in homozygous wandering third instar larvae and white prepupae.

Homozygous larvae have a significantly increased triglyceride content compared to controls.

Generation of Cdk8K185 clones specifically in the leg (using Scer\GAL4Dll-em212 and Scer\FLP1Scer\UAS.cUa) and in a Minute background results in severely stunted legs. In an other wild type background, these clones result in tarsal segmentation defects in all three paris of adult legs. Male prothoracic (T1) legs exhibit sex combs with slightly elevated teeth number and discontinuities in the tooth alignment, leading to irregularly grouped, fragmented sex combs. Leg size is relatively unaffected, but the most proximal tarsal joint is usually abnormal.

Generation of large Cdk8K185 clones specifically in the eye (using Scer\FLP1ey.PB) in a Minute background results in full-sized eyes with mostly normal differentiated ommatidia.

Heat-induced Cdk8K185 clones in the adult notum are associated with loss of bristles.

Generation of large Cdk8K185 clones specifically in the eye (using Scer\GAL4ap-md544 and Scer\FLP1Scer\UAS.cUa) in a Minute background results in extensive loss of notal macrochaetes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The phenotypes of Cdk8K185 CycCY5 double mutants are similar to those of the single mutants.

The distal leg phenotypes provoked by Cdk8K185, ktoT241 double mutant clones induced specifically in the leg (using Scer\GAL4Dll-em212 and Scer\FLP1Scer\UAS.cUa) are indistinguishable from ktoT241 alone but distinct from Cdk8K185 alone, both for sex comb differentiation and for overall leg structure.

The phenotypes of Cdk8K185, ktoT241 double mutant clones induced specifically in the eye (using Scer\FLP1ey.PB) in a Minute background are indiscernible from ktoT241 alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Cdk8T:Avic\GFP-EGFP rescues the developmental delay to pupariation, the failure of prepupal anterior spiracle eversion and the prepupal lethality of Cdk8K185 animals.

Cdk8K185 homozygotes are fully rescued to yield viable, morphologically normal, fertile adults by expression of Cdk8Ubi-p63E.PL.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)