Pupariation is delayed by approximately 2-3 days in homozygous animals. This developmental delay is slightly rescued by feeding with 20-hydroxyecdysone.
96% of homozygous prepupae fail to evert their anterior spiracles, and the prepupae are partially separated from the pupal case.
The level of ecdysteroids is significantly lower than normal in homozygous wandering third instar larvae and white prepupae.
Homozygous larvae have a significantly increased triglyceride content compared to controls.
Generation of Cdk8K185 clones specifically in the leg (using Scer\GAL4Dll-em212 and Scer\FLP1Scer\UAS.cUa) and in a Minute background results in severely stunted legs. In an other wild type background, these clones result in tarsal segmentation defects in all three paris of adult legs. Male prothoracic (T1) legs exhibit sex combs with slightly elevated teeth number and discontinuities in the tooth alignment, leading to irregularly grouped, fragmented sex combs. Leg size is relatively unaffected, but the most proximal tarsal joint is usually abnormal.
Generation of large Cdk8K185 clones specifically in the eye (using Scer\FLP1ey.PB) in a Minute background results in full-sized eyes with mostly normal differentiated ommatidia.
Heat-induced Cdk8K185 clones in the adult notum are associated with loss of bristles.
Generation of large Cdk8K185 clones specifically in the eye (using Scer\GAL4ap-md544 and Scer\FLP1Scer\UAS.cUa) in a Minute background results in extensive loss of notal macrochaetes.