Allele Dmel\Fer2LCH^Scer\UAS.cMa

General Information
Symbol	Dmel\Fer2LCH^Scer\UAS.cMa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Missirlis	FlyBase ID	FBal0218476
Feature type	allele	Associated gene	Dmel\Fer2LCH

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Missirlis et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the Fer2LCH LD01936 cDNA. (Missirlis et al., 2007)
Carried in construct	P{UAS-Fer2LCH.M} (Missirlis et al., 2007, Kosmidis et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	circadian rhythm defective, with Scer\GAL4^nrv2.PS (Kosmidis et al., 2011) circadian rhythm defective, with Scer\GAL4^repo (Kosmidis et al., 2011)
Phenotype Manifest In

Detailed Description
	Statement Reference Fer2LCH[Scer\UAS.cMa] overexpression in all adult brain glia under the control of Scer\GAL4[repo] does not generate statistically significant differences in escape response in the first four weeks of adult life. Overexpression of Fer2LCH[Scer\UAS.cMa] in all adult brain glia under the control of Scer\GAL4[repo] results in a circadian phenotype in 54% of flies. (Kosmidis et al., 2011) Expression of Fer2LCH^Scer\UAS.cMa alone under the control of Scer\GAL4^Act does not result in increased resistance to paraquat compared to control flies. (Missirlis et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Fer2LCH^Scer\UAS.cMa, Scer\GAL4^repo has circadian rhythm defective phenotype, enhanceable by Fer1HCH^Scer\UAS.cMa, Scer\GAL4^repo (Kosmidis et al., 2011)
Enhancer of
Statement Reference Fer2LCH^Scer\UAS.cMa, Scer\GAL4^repo is an enhancer of circadian rhythm defective phenotype of Fer1HCH^Scer\UAS.cMa, Scer\GAL4^repo (Kosmidis et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^Act.PU/Fer2LCH^Scer\UAS.cMa is a non-suppressor of lethal phenotype of Fer1HCH⁰⁰⁴⁵¹ (Missirlis et al., 2007) Scer\GAL4^Act.PU/Fer2LCH^Scer\UAS.cMa is a non-suppressor of lethal phenotype of Fer1HCH^G00188/Fer1HCH⁰⁰⁴⁵¹ (Missirlis et al., 2007)
Other
Statement Reference Fer1HCH^mut.Scer\UAS, Fer2LCH^Scer\UAS.cMa, Scer\GAL4^Act.PU has chemical resistant \| female limited phenotype (Missirlis et al., 2007) Fer1HCH^Scer\UAS.cMa, Fer2LCH^Scer\UAS.cMa, Scer\GAL4^Act.PU has chemical resistant \| female limited phenotype (Missirlis et al., 2007)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Flies simultaneously expressing Fer1HCH[Scer\UAS.cMa] and Fer2LCH[Scer\UAS.cMa] in glia under the control of Scer\GAL4[repo] exhibit an abrupt and pronounced deficit in escape responses in both horizontal and vertical directions at five and six weeks of age. Quantification of rhythmic patterns shows that 57% of six-week old individuals overexpressing both Fer1HCH[Scer\UAS.cMa] and Fer2LCH[Scer\UAS.cMa] in glia under the control of Scer\GAL4[repo] remain rhythmic in constant darkness, albeit with a reduced value for rhythmicity statistic, suggesting behavioral disturbances even in the flies categorized as rhythmic. Three-week old flies overexpressing both chains in glia show robut rhythms in 69% of the individuals tested. Approximately 63% of six-week old Scer\GAL4[nrv2.PS]/Fer1HCH[Scer\UAS.cMa], Fer2LCH[Scer\UAS.cMa] flies are rhythmic compared to 91% for similar aged Scer\GAL4[nrv2.PS]/+ flies. Flies overexpressing both Fer1HCH[Scer\UAS.cMa] and Fer2LCH[Scer\UAS.cMa] in glia under the control of Scer\GAL4[repo] or Scer\GAL4[nrv2.PS] accumulate ferritin-iron inclusions in the outermost layer of the medulla and in the lamina in the optic lobe, with crystal-like (i.e. symmetric) morphology and are surrounded by an intracellular membrane. Flies overexpressing both Fer1HCH[Scer\UAS.cMa] and Fer2LCH[Scer\UAS.cMa] in glia under the control of Scer\GAL4[repo] or Scer\GAL4[nrv2.PS] do not exhibit any apparent neurodegeneration or a shorter lifespan compared to controls. (Kosmidis et al., 2011) Co-expression of both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^Act results in females with greater resistance to paraquat than wild-type controls (males show the same sensitivity to paraquat as controls). Co-expression of both Fer1HCH^mut.Scer\UAS and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^Act results in females with greater resistance to paraquat than wild-type controls (males show the same sensitivity to paraquat as controls). Males co-expressing both Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa under the control of Scer\GAL4^Act are at a developmental disadvantage compared to their control siblings, with this effect being most pronounced under iron-limiting conditions and with stronger overexpression of Fer1HCH^Scer\UAS.cMa and Fer2LCH^Scer\UAS.cMa. (Missirlis et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^Act.PU/Fer2LCH^Scer\UAS.cMa rescues Fer2LCH⁰⁰⁰³⁵ (Missirlis et al., 2007)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Fer2LCH^Scer\UAS.cMa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Missirlis
Secondary FlyBase IDs

References ( 2 )
Research paper	Kosmidis et al., 2011, Neurobiol. Disease 43(1): 213--219 Ferritin overexpression in Drosophila glia leads to iron deposition in the optic lobes and late-onset behavioral defects. [FBrf0213691] Missirlis et al., 2007, Genetics 177(1): 89--100 Homeostatic mechanisms for iron storage revealed by genetic manipulations and live imaging of Drosophila ferritin. [FBrf0202180]

Allele Dmel\Fer2LCHScer\UAS.cMa

Allele Dmel\Fer2LCH^Scer\UAS.cMa