FlyBase Allele Report: Dmel\sub[ΔNT.UASp.EGFP]

General Information

Symbol

Dmel\sub^{ΔNT.UASp.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0218574

Feature type

allele

Associated gene

Dmel\sub

Associated Insertion(s)

Carried in Construct

P{UASp-sub.ΔNT}

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Jang et al., 2007)

in vitro construct - coding region fusion

(Jang et al., 2007)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UASp-sub.ΔNT}

Cytology

Nature of the lesion

Statement

Reference

UASp regulatory sequences drive expression of a mutated form of sub, which lacks the N-terminal domain, and which is tagged with EGFP.

(Jang et al., 2007)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

sub

Tagged with

EGFP

green fluorescent protein

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^VP16.nos.UTR

(Cesario and McKim, 2011)

Phenotype Manifest In

karyosome, with Scer\GAL4^VP16.nos.UTR

(Jang et al., 2007)

oocyte, with Scer\GAL4^VP16.nos.UTR

(Cesario and McKim, 2011)

oocyte | oogenesis stage S14, with Scer\GAL4^VP16.nos.UTR

(Jang et al., 2007)

spindle | ectopic, with Scer\GAL4^VP16.nos.UTR

(Cesario and McKim, 2011)

spindle microtubule | oogenesis stage S14, with Scer\GAL4^VP16.nos.UTR

(Jang et al., 2007)

Detailed Description

Statement

Reference

Expression of sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in the formation of ectopic spindles in 98% of oocytes. The ectopic spindles cluster and form in many regions in the oocyte without direct contact with chromosomes.

(Cesario and McKim, 2011)

Stage 14 oocytes expressing sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} contain a large number of bundled microtubules that appear like bipolar spindles. Most of these "ectopic" spindles are not associated with chromosomes. The karyosomes are usually seen to have prematurely split into at least two masses of chromosomes that are scattered in the oocyte cytoplasm. These ectopic spindles are not seen in stage 13 or earlier stage oocytes.

(Jang et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^VP16.nos.UTR, sub^{ΔNT.UASp.EGFP} has female sterile phenotype, suppressible | partially by Scer\GAL4^VP16.nos.UTR/Ran^{T24N.UASp.Tag:HA}

(Cesario and McKim, 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^VP16.nos.UTR, sub^{ΔNT.UASp.EGFP} has female sterile phenotype, non-suppressible by Ran^UASp.Tag:HA/Scer\GAL4^VP16.nos.UTR

(Cesario and McKim, 2011)

Suppressor of

Statement

Reference

sub^{ΔNT.UASp.EGFP}/Scer\GAL4^VP16.nos.UTR is a suppressor | partially of female sterile phenotype of Scer\GAL4^VP16.nos.UTR, Ran^{T24N.UASp.Tag:HA}

(Cesario and McKim, 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^VP16.nos.UTR, sub^{ΔNT.UASp.EGFP} has spindle | ectopic phenotype, suppressible by Scer\GAL4^VP16.nos.UTR/Ran^{T24N.UASp.Tag:HA}

(Cesario and McKim, 2011)

Scer\GAL4^VP16.nos.UTR, sub^{ΔNT.UASp.EGFP} has oocyte phenotype, suppressible by Scer\GAL4^VP16.nos.UTR/Ran^{T24N.UASp.Tag:HA}

(Cesario and McKim, 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^VP16.nos.UTR, sub^{ΔNT.UASp.EGFP} has spindle | ectopic phenotype, non-suppressible by Ran^UASp.Tag:HA/Scer\GAL4^VP16.nos.UTR

(Cesario and McKim, 2011)

Scer\GAL4^VP16.nos.UTR, sub^{ΔNT.UASp.EGFP} has oocyte phenotype, non-suppressible by Ran^UASp.Tag:HA/Scer\GAL4^VP16.nos.UTR

(Cesario and McKim, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of the ectopic spindle phenotype seen in the oocytes of females expressing sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is not suppressed by co-expression of ran^{Scer\UAS.P\T.T:Ivir\HA1}.

Co-expression of ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} and sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} increases female fertility compared to the fertility of females expressing either ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} or sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} separately.

The ectopic spindle phenotype seen in the oocytes of females expressing sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is completely suppressed by co-expression of ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}.

The arrest in embryonic development seen in zygotes derived from females expressing ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is partially suppressed by co-expression of sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP}.

(Cesario and McKim, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP}

sub^{ΔNT.UASp.EGFP}

sub^{ΔNT.UASp.T:Avic-EGFP}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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