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General Information
Symbol
Dmel\subΔNT.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0218574
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of a mutated form of sub, which lacks the N-terminal domain, and which is tagged with EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of subΔNT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in the formation of ectopic spindles in 98% of oocytes. The ectopic spindles cluster and form in many regions in the oocyte without direct contact with chromosomes.

Stage 14 oocytes expressing subΔNT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 contain a large number of bundled microtubules that appear like bipolar spindles. Most of these "ectopic" spindles are not associated with chromosomes. The karyosomes are usually seen to have prematurely split into at least two masses of chromosomes that are scattered in the oocyte cytoplasm. These ectopic spindles are not seen in stage 13 or earlier stage oocytes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The frequency of the ectopic spindle phenotype seen in the oocytes of females expressing subΔNT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is not suppressed by co-expression of ranScer\UAS.P\T.T:Ivir\HA1.

Co-expression of ranT24N.Scer\UAS.P\T.T:Ivir\HA1 and subΔNT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 increases female fertility compared to the fertility of females expressing either ranT24N.Scer\UAS.P\T.T:Ivir\HA1 or subΔNT.Scer\UAS.P\T.T:Avic-EGFP separately.

The ectopic spindle phenotype seen in the oocytes of females expressing subΔNT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is completely suppressed by co-expression of ranT24N.Scer\UAS.P\T.T:Ivir\HA1.

The arrest in embryonic development seen in zygotes derived from females expressing ranT24N.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is partially suppressed by co-expression of subΔNT.Scer\UAS.P\T.T:Avic-EGFP.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
subΔNT.Scer\UAS.P\T.T:Avic-EGFP
subΔNT.UASp.EGFP
subΔNT.UASp.T:Avic-EGFP
Name Synonyms
Secondary FlyBase IDs
    References (3)