cry01, Rh70 double-homozygous adults reared under 12h:12h white light:dark cycles exhibit: an earlier morning activity onset as compared to controls, which is more severe than both single mutant; an earlier evening activity onset as compared to controls, instead of the later evening activity onset observed in cry01 mutants; and a longer siesta as compared to controls, which is slightly shorter than that of cry01 mutants. These double mutants show an apparently normal resynchronization to a 6h delay in 12h:12h white light:dark cycle and an apparently normal rhythmicity under constant dark, as compared to both single mutants and to wild-type controls.
cry01, norpA39 and Rh70, norpA39 double-homozygous adults reared under 12h:12h white light:dark cycles exhibit a significant delay in resynchronizing to a 6h delay in 12h:12h white light:dark cycle compared to controls; these phenotypes are not enhanced by additional cry01 and Rh70 homozygosity, respectively.
cry01, norpA36 double homozygous adults previously trained under 12h:12h light:dark cycles present slightly increased circadian rhythmicity defects under constant darkness conditions, which are rescued by additional homozygosity for either as trpMB03672 or trplMB10553, compared to each single homozygotes.
cry01, Rh71 double homozygous adults exhibit an increased delay in circadian phase shifting to a 8h delay in the light:dark transition, as compared to either single homozygote. A significant proportion of either cry01/cry01, Rh71/Rh71 adults or cry01/cryb, Rh71/Rh71 adults previously trained under 12h:12h light:dark cycles fail to maintain rhythmicity under constant darkness conditions, while the rhythmic individuals present a significantly longer period, as compared to controls.
norpA36 cry01 double mutant flies generally fail to resynchronise to the new light regime when they are entrained to 12 hour light: 12 hour dark cycles and then exposed to 8 hour delayed light-dark (LD) cycles; their daily peaks of locomotion continue to occur at the time of the photic transitions that that were in operation before the 8 hour shift. Some of the double mutant flies are "partially entrained"; six says after the photic shift, daily peaks of locomotion fall between the phases expected for continuing entrainment to the old LD regime versus re-entrainment to the new one.
norpA36 cry01 double mutant flies are impaired in their ability to re-entrain with novel non-24 hour light:dark (LD) cycles (compared to single mutant flies) when they are entrained to 12:12 LD and are then shifted to 9:9 , 13:13 or 14:14 LD cycles.