FB2025_01 , released February 20, 2025
Allele: Dmel\EndoGMB07150
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General Information
Symbol
Dmel\EndoGMB07150
Species
D. melanogaster
Name
FlyBase ID
FBal0218782
Feature type
allele
Associated gene
Dmel\EndoG
Associated Insertion(s)
Mi{ET1}EndoGMB07150
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    Mi{ET1}EndoGMB07150
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    Mi{ET1}
    Encoded product / tool
    GAL4
    Mutations Mapped to the Genome
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        EndoGMB07150/Df(2R)BSC699 transheterozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to controls.

        (Napoletano et al., 2017)

        EndoGMB07150 mutants show defects in mitochondrial nucleoid elimination during spermatid development.

        (Yu et al., 2017)

        60% less spermatogonial cyst death is seen in EndoGMB07150 mutant males in comparison with wild type controls.

        (Yacobi-Sharon et al., 2013)

        In EndoGMB07150 mutant spermatid bundles, mitochondrial DNA nucleoids fail to be eliminated from the apical portion of sperm tail bundles during spermatid elongation and persist throughout spermatid individualisation. Nucleoids are found ahead of the cystic bulge but not behind it and accumulate in the cystic body as it traverses regions of the tail with residual mtDNA. However the number of nucleoids collected by the cystic body never approaches the number per bundle, even though none are left behind. The number of nucleoids accumulated in the cystic body is slightly increased in EndoGMB07150/Df(2R)BSC699 compared to homozygous EndoGMB07150.

        (Deluca and O'Farrell, 2012)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Other
        Statement
        Reference

        EndoGMB07150, p53[+]/p53E8 has hyperplasia | spermatogenesis phenotype

        (Napoletano et al., 2017)
        Phenotype Manifest In
        NOT Enhanced by
        NOT Enhancer of
        Other
        Statement
        Reference

        EndoGMB07150, p53[+]/p53E8 has testis | adult stage phenotype

        (Napoletano et al., 2017)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        EndoGMB07150/Df(2R)BSC699, p53E8/+ adults exhibit a significant increase in the proportion of hyperplastic testes, as compared to EndoGMB07150/Df(2R)BSC699 adults, p53E8/+ adults, or wild-type controls.

        (Napoletano et al., 2017)

        The failure to eliminate mitochondrial nucleoids during spermatid development characteristic for males expressing tamdsRNA.UAS.cUa under the control of Scer\GAL4bam.T:Hsim\VP16 cannot be exacerbated further by combination with EndoGMB07150.

        (Yu et al., 2017)

        Loss of the 4 EndoG orthologs (Tengl1 Tengl2 Tengl3 and Tengl4) using Df(2L)TENGL1-3 and Df(3R)TENGL41/Df(3R)TENGL42 does not enhance the mitochondrial nucleoid elimination defects seen in EndoGMB07150 mutant spermatids.

        (Deluca and O'Farrell, 2012)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        EndoGMB07150 is rescued by Scer\GAL4VP16.nanos.UTR/EndoGUASp.Tag:MYC

        (Deluca and O'Farrell, 2012)
        Comments

        Expression of EndoGScer\UAS.P\T.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the defects in mitochondrial nucleoid elimination seen in EndoGMB07150 mutant spermatids.

        (Deluca and O'Farrell, 2012)
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        Comments
        Comments

        Precise excision of the P{EP} element in Mi{ET1}EndoGMB07150 reverts the nucleoid phenotype.

        (Deluca and O'Farrell, 2012)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        CG8862MB07150
        (Bellen, 2008.11.7)
        EndoGMB07150
        (Napoletano et al., 2017, Yu et al., 2017, Deluca and O'Farrell, 2012)
        endoGMB07150
        (Tarayrah-Ibraheim et al., 2021, Yacobi-Sharon et al., 2013)
        Name Synonyms
        Secondary FlyBase IDs
          References (6)