Allele Dmel\gwl^Sr6

General Information
Symbol	Dmel\gwl^Sr6	Species	D. melanogaster
Name		FlyBase ID	FBal0218849
Feature type	allele	Associated gene	Dmel\gwl

Allele class	hypomorphic allele - genetic evidence
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Archambault et al., 2007)
Mutagen	X ray (Archambault et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference In addition to the amino acid substitution, gwl[Sr6] has a K689STOP substitution. (Archambault et al., 2007) Amino acid replacement: K689@. (Archambault et al., 2007) Amino acid replacement: K97M. (Archambault et al., 2007) Nucleotide substitution: A?T. (Archambault et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	eclosion rhythm defective (with gwl^Sr3) (Archambault et al., 2007) female fertile (with gwl²) (Archambault et al., 2007) female fertile (with gwl^3a) (Archambault et al., 2007) female fertile (with gwl^6a) (Archambault et al., 2007) fertile (with gwl²) (Archambault et al., 2007) fertile (with gwl^3a) (Archambault et al., 2007) fertile (with gwl^6a) (Archambault et al., 2007) mitotic cell cycle defective (with gwl^Sr3) (Archambault et al., 2007) partially lethal - majority live (with gwl^Sr3) (Archambault et al., 2007) sterile (Archambault et al., 2007)
Phenotype Manifest In
	chromatin (Archambault et al., 2007) chromatin (with gwl^Sr3) (Archambault et al., 2007) eye (with gwl^Sr3) (Archambault et al., 2007) gonad (with gwl^Sr3) (Archambault et al., 2007) macrochaeta (with gwl^Sr3) (Archambault et al., 2007) wing margin (with gwl^Sr3) (Archambault et al., 2007)
Detailed Description
	Statement Reference gwl[Sr6] homozygotes die as pupae (although this is believed to reflect a second lethal on its chromosome). gwl[Sr6] mutants are sterile in both males and females. gwl[Sr3]/gwl[6a] mutants exhibit mitotic defects, leading to an increase in the mitotic index with more cells in anaphase. Mutant neuroblasts show a high frequency of defective chromatin condensation. Some regions of chromatin appear undercondensed and other regions are overcondensed. In some cells the chromosomes are uniformly overcondensed, suggesting a lengthy pre-anaphase delay. gwl[Sr3]/gwl[Sr6] adults eclose (after a delay), with variably rough eyes, eroded wing margins, missing bristles and strongly reduced gonads, though viability is reduced. gwl[Sr6]/Df(3L)gwl[3b] mutants die predominantly in the pupal stage, and the rare escaper adults have rough eyes, ragged wings, cuticle defects, missing bristles, and are sterile. gwl[3a]/gwl[Sr6] mutants produce a gwl[Sr3] phenotype, i.e female fertility is restored. gwl[2]/gwl[Sr6] mutants produce a gwl[Sr3] phenotype, i.e female fertility is restored. gwl[6a]/gwl[Sr6] mutants produce a gwl[Sr3] phenotype, i.e female fertility is restored. (Archambault et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	gwl^Sr6/Df(3L)gwl^3b is rescued by Scer\GAL4^Act5C.PU/gwl^{K97M.Scer\UAS} (Archambault et al., 2007) gwl^Sr6/Df(3L)gwl^3b is rescued by Scer\GAL4^Act5C.PU/gwl^L.Scer\UAS (Archambault et al., 2007) gwl^Sr6/gwl^Sr3 is rescued by Scer\GAL4^Act5C.PU/gwl^{K97M.Scer\UAS} (Archambault et al., 2007) gwl^Sr6/gwl^Sr3 is rescued by Scer\GAL4^Act5C.PU/gwl^L.Scer\UAS (Archambault et al., 2007)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Based on viability, the following gwl alleles can be ranked from strongest to weakest as follows: gwl[Sr3] > gwl[Sr3]/Df(3L)gwl[3b] > gwl[Sr6] > gwl[Sr6]/Df(3L)gwl[3b]> gwl[Sr3]/gwl[Sr6] > gwl[Sr18]/Df(3L)gwl[3b]. (Archambault et al., 2007)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	gwl^Sr6 (Archambault et al., 2007)
Name Synonym	Scant revertant (Archambault et al., 2007)
Secondary FlyBase IDs

References ( 1 )
Research paper	Archambault et al., 2007, PLoS Genet. 3(11): e200 Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence with polo kinase. [FBrf0202769]

Allele Dmel\gwlSr6

Allele Dmel\gwl^Sr6