Allele Dmel\gwl^L.Scer\UAS

General Information
Symbol	Dmel\gwl^L.Scer\UAS	Species	D. melanogaster
Name	UAS construct of the Long isoform of greatwall	FlyBase ID	FBal0218854
Feature type	allele	Associated gene	Dmel\gwl

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Archambault et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the gwl coding sequences corresponding to the long isoform (required for female meiosis). (Archambault et al., 2007)
Carried in construct	P{UASp-gwl.Long} (Archambault et al., 2007, Wang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Female germline expression of gwl[L.Scer\UAS] (under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16]) has no effect on fertility, with embryos appearing wild-type. (Archambault et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference gwl^L.Scer\UAS/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is a suppressor of sterile phenotype of polo¹¹ (Archambault et al., 2007)
Other
Statement Reference Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, gwl^L.Scer\UAS, tws^j11C8 has meiotic cell cycle defective \| dominant phenotype (Wang et al., 2011) Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, gwl^L.Scer\UAS, tws^j11C8 has sterile \| dominant phenotype (Wang et al., 2011)
Phenotype Manifest In
Enhancer of
Statement Reference gwl^L.Scer\UAS/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is an enhancer of centrosome phenotype of polo¹¹ (Archambault et al., 2007)
Suppressor of
Statement Reference gwl^L.Scer\UAS/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is a suppressor of egg phenotype of polo¹¹ (Archambault et al., 2007) gwl^L.Scer\UAS/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is a suppressor of spindle phenotype of polo¹¹ (Archambault et al., 2007)
Other
Statement Reference Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, gwl^L.Scer\UAS, tws^j11C8 has spindle \| embryonic stage phenotype (Wang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Overexpression of gwl[L.Scer\UAS] in eggs/embryos under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16] from tws[j11C8]/+ mothers is almost completely lethal. Most eggs/embryos are blocked in metaphase of meiosis I, even after 4 to 6 hours post-laying. Only a few eggs manage to complete meiosis and attempt to initiate embryonic development. However, these embryos usually abort in the first mitosis, displaying several aberrant structures with condensed chromatin at the center of small spindles. The few mitotic nuclei observed show a very high incidence of detached centrosomes. (Wang et al., 2011) Female germline expression of gwl[L.Scer\UAS] (under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16]) in polo[11] heterozygous females allows partial egg hatch (around 4%), and these embryos also show loss of centrosomes from spindles in early syncytial divisions. (Archambault et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	gwl^L.Scer\UAS/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues Df(3L)gwl^3b/gwl^Sr18 (Archambault et al., 2007) Scer\GAL4^Act5C.PU/gwl^L.Scer\UAS rescues Df(3L)gwl^3b/gwl^Sr3 (Archambault et al., 2007) Scer\GAL4^Act5C.PU/gwl^L.Scer\UAS rescues gwl^Sr3 (Archambault et al., 2007) Scer\GAL4^Act5C.PU/gwl^L.Scer\UAS rescues gwl^Sr6/Df(3L)gwl^3b (Archambault et al., 2007) Scer\GAL4^Act5C.PU/gwl^L.Scer\UAS rescues gwl^Sr6/gwl^Sr3 (Archambault et al., 2007)
Comments	Expression of gwl[L.Scer\UAS] under the control of Scer\GAL4[Act5C] rescues the viability and somatic integrity of gwl[Sr3]/gwl[Sr6], gwl[Sr3]/gwl[Sr3], gwl[Sr3]/Df(3L)gwl[3b] and gwl[Sr3]/Df(3L)gwl[3b] flies to similar degrees. The fertility of gwl[Sr18]/Df(3L)gwl[3b] females is rescued completely by expression of gwl[L.Scer\UAS] in the maternal germline with Scer\GAL4[mat.αTub67C.T:Hsim\VP16]. (Archambault et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	gwl^L.Scer\UAS
Name Synonym	UAS construct of the Long isoform of greatwall
Secondary FlyBase IDs

References ( 2 )
Research paper	Wang et al., 2011, PLoS Genet. 7(8): e1002227 PP2A-Twins Is Antagonized by Greatwall and Collaborates with Polo for Cell Cycle Progression and Centrosome Attachment to Nuclei in Drosophila Embryos. [FBrf0214683] Archambault et al., 2007, PLoS Genet. 3(11): e200 Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence with polo kinase. [FBrf0202769]

Allele Dmel\gwlL.Scer\UAS

Allele Dmel\gwl^L.Scer\UAS