FB2025_01 , released February 20, 2025
Allele: Dmel\Vha68-2R6
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General Information
Symbol
Dmel\Vha68-2R6
Species
D. melanogaster
Name
FlyBase ID
FBal0219044
Feature type
allele
Associated gene
Dmel\Vha68-2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Menut et al., 2007)
Progenitor genotype
Cytology
Description

Introduces a stop codon in the region encoding the ATP synthetase α/β chain of Vha68-2.

(Vaccari et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:12,977,885..12,977,885 [+]
Nucleotide change:

C12977885G

Reported nucleotide change:

C?G

Amino acid change:

Y576term | Vha68-2-PA; Y576term | Vha68-2-PB; Y576term | Vha68-2-PC; Y576term | Vha68-2-PD; Y576term | Vha68-2-PE; Y576term | Vha68-2-PF

Reported amino acid change:

Y?term

Comment:

Reported as a mutaton from NIMYQLS to NIM@ in the penultimate exon.

(Vaccari et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Induction of Vha68-2R6 homozygous whole-eye clones in the third instar larva leads to the absence of eyes in adults.

      (Zhao et al., 2018)

      Wing hair polarity is mostly unaffected in homozygous clones at 32-33 hours after puparium formation.

      (Hermle et al., 2013)

      Predominantly mutant Vha68-2R6 eye discs (generated using Scer\FLP1ey.PN) are small in size compared to wild type and have aberrant morphology and compromised epithelial polarity.

      Vha68-2R6 eye disc cells show an increased number of multivesicular bodies (MVBs) compared to wild type. These MVBs are enlarged and contain a high number of luminal vesicles.

      Endocytic organelles are present in Vha68-2R6 homozygous mosaic eye disc clones but the cells have a reduced number of acidified endosomes compared to wild type.

      A portion of the cells in Vha68-2R6 mosaic clones are eliminated from the disc, especially when surrounded by wild type cells.

      Vha68-2R6 mosaic eye disc clones underproliferate compared to controls.

      Vha68-2R6 homozygous mutant follicle cell mosaic clones have a reduced number of acidified endosomes compared to wild type.

      (Vaccari et al., 2010)

      Mutant eye discs (generated using the eyFLP-cell lethal system) show disrupted epithelial organisation.

      (Menut et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Vha68-2[+]/Vha68-2R6 is a suppressor | partially of visible | adult stage phenotype of MitfUAS.cZa, Scer\GAL4dpp.blk1

      (Zhang et al., 2015)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Vha68-2[+]/Vha68-2R6 is a suppressor | partially of wing | adult stage phenotype of MitfUAS.cZa, Scer\GAL4dpp.blk1

      (Zhang et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The small underdeveloped wing characteristic for adult flies expressing MitfScer\UAS.cZa under the control of Scer\GAL4dpp.blk1 is significantly suppressed by combination with a single copy of Vha68-2R6.

      (Zhang et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      MENE-R6R6
      MENE2L-R6
      (Vaccari et al., 2010)
      R6
      (Menut et al., 2007)
      Vha68-2R6
      (Portela et al., 2018, Hermle et al., 2013, Vaccari et al., 2010)
      vha68-2R6
      (Wang et al., 2021, Zhao et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)