Allele Dmel\Gadd45^{Scer\UAS.P\T.cPa}

General Information
Symbol	Dmel\Gadd45^{Scer\UAS.P\T.cPa}	Species	D. melanogaster
Name		FlyBase ID	FBal0219054
Feature type	allele	Associated gene	Dmel\Gadd45

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Peretz et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of the entire Gadd45 coding region. (Peretz et al., 2007)
Carried in construct	P{UASp-Gadd45.P} (Peretz et al., 2007, Plyusnina et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act5C.PU (Peretz et al., 2007) lethal, with Scer\GAL4^tub.PU (Peretz et al., 2007) locomotor rhythm defective (Plyusnina et al., 2011) locomotor rhythm defective \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Plyusnina et al., 2011) long lived (Plyusnina et al., 2011) long lived \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Plyusnina et al., 2011)
Phenotype Manifest In
	chorion, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Peretz et al., 2007) dorsal appendage, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Peretz et al., 2007) egg chamber, with Scer\GAL4^CY2 (Peretz et al., 2007) follicle cell, with Scer\GAL4^CY2 (Peretz et al., 2007) nurse cell, with Scer\GAL4^CY2 (Peretz et al., 2007)
Detailed Description
	Statement Reference Constitutive overexpression of Gadd45[Scer\UAS.P\T.cPa] under the control of Scer\GAL4[1407] results in a lifespan increase in both males and females compared to controls. The median lifespan of males with constitutive Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous system is extended by 77 and 73% compared to the median lifepsan of males of the parental Gadd45[Scer\UAS.P\T.cPa] and Scer\GAL4[1407] strains, respectively. The age of 90% mortality of males with constitutive Gadd45[Scer\UAS.P\T.cPa] overexpression is increased by 25 and 59% compared to parental Gadd45[Scer\UAS.P\T.cPa] and Scer\GAL4[1407] males, and in females by 11 and 50%, respectively. This indicates that constitutive Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous system increases lifespan and delays aging in males and females. This effect is larger in males than in females. Survival curves demonstrate a lifespan increase in males expressing Gadd45[Scer\UAS.P\T.cPa] in neuronal cells under the control of Scer\GAL4[elav.Switch.PO] that are treated with mifepristone compared to those kept with mifepristone and males of parental strains. Specifically, the median lifespan of males with mifepristone-inducible Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous sytem is higher by 40-42% compared to males with the same genotype not treated with mifepristone, while in females, by 3-6%. The median lifespan of males with conditional Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous is increased by 50-70 and 62-84%, compared to the median lifespan of parental Gadd45[Scer\UAS.P\T.cPa] and Scer\GAL4[elav.Switch.PO] strain males, respectively, and in females by 27-29 and 100-102%, respectively. In females with constitutive Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous system (under the control of Scer\GAL4[1407]), fecundity is higher over the entire course of lifetime, compared to the parental strain females. The average number of eggs laid daily by a female increases 2-2.3-fold compared to parental strains, while the number of pupae per female is 3.7 and 4.4 times higher, respectively. In females with Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous system (under the control of Scer\GAL4[elav.Switch.PO]), the average number of eggs laid daily increase 1.7 times compared to the Scer\GAL4[elav.Switch.PO] parental strain. The locomotor activity of males and females with constitutive Gadd45[Scer\UAS.P\T.cPa] overexpression in the nervous system (under the control of Scer\GAL4[1407]) is increased compared with males and females of both parental strains throughout their whole lifetime, both in terms of spontaneous locomotor activity and according to a negative geotaxis test. There is an increase in locomotor activity in males (on day 4-29) and in females (on day 12-36) with conditional expression of Gadd45[Scer\UAS.P\T.cPa] under the control of Scer\GAL4[elav.Switch.PO]. In the neuroblasts of larvae constitutively or conditionally expressing Gadd45[Scer\UAS.P\T.cPa] in the nervous system under the control of Scer\GAL4[1407] or Scer\GAL4[elav.Switch.PO] respectively, the DNA damage level is reduced by 21-27%, with statistical significance compared to control larvae. However, the level of apoptosis seen in these larval neuroblasts does not differ significantly. (Plyusnina et al., 2011) Expression of Gadd45[Scer\UAS.P\T.cPa] under the control of Scer\GAL4[CY2] results in apoptosis of the egg chamber, shown by condensation and breaking up of DNA within both the nurse and follicle cells. Expression of Gadd45[Scer\UAS.P\T.cPa] under the control of Scer\GAL4[GMR.PF] does not result in any gross morphological changes in the eye. Expression of Gadd45[Scer\UAS.P\T.cPa] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] results in a broad range of abnormal eggshell phenotypes, ranging from normal morphology to dorsalised. Weakly dorsalised eggs have two broadened dorsal appendages, while moderately dorsalised eggshells have one broadened dorsal appendage on the dorsal midline. A small fraction of the eggs have a more posteriorly located patch of dorsal appendage material. (Peretz et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Gadd45^{Scer\UAS.P\T.cPa}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has chorion phenotype, suppressible by Df(1)H6 (Peretz et al., 2007) Gadd45^{Scer\UAS.P\T.cPa}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has chorion phenotype, suppressible by hep[+]/hep^r75 (Peretz et al., 2007) Gadd45^{Scer\UAS.P\T.cPa}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has chorion phenotype, suppressible by hep^G0208/hep[+] (Peretz et al., 2007) Gadd45^{Scer\UAS.P\T.cPa}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has dorsal appendage phenotype, suppressible by Df(1)H6 (Peretz et al., 2007) Gadd45^{Scer\UAS.P\T.cPa}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has dorsal appendage phenotype, suppressible by hep[+]/hep^r75 (Peretz et al., 2007) Gadd45^{Scer\UAS.P\T.cPa}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has dorsal appendage phenotype, suppressible by hep^G0208/hep[+] (Peretz et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference The dorsalised eggshell phenotype caused by expression of Gadd45[Scer\UAS.P\T.cPa] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] is significantly suppressed by Df(1)H6, hep[G0208]/+ or hep[r75]/+. (Peretz et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Gadd45^{Scer\UAS.P\T.cPa}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Plyusnina et al., 2011, Biogerontology 12(3): 211--226 Increase of Drosophila melanogaster lifespan due to D-GADD45 overexpression in the nervous system. [FBrf0213783] Peretz et al., 2007, Genetics 177(3): 1691--1702 Expression of the Drosophila melanogaster GADD45 homolog (CG11086) affects egg asymmetric development that is mediated by the c-Jun N-terminal kinase pathway. [FBrf0201501]

Allele Dmel\Gadd45Scer\UAS.P\T.cPa

Allele Dmel\Gadd45^{Scer\UAS.P\T.cPa}