FlyBase Allele Report: Dmel\Egfr[DN.cUa.UAS]

General Information

Symbol

Dmel\Egfr^DN.cUa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0219094

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{UAS-Egfr.DN.U}

Also Known As

UAS-Egfr^DN

Key Links

Transgenic product class

dominant_negative_variant

(Overton et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Overton et al., 2007)

Mutagen

in vitro construct

(Overton et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Egfr.DN.U}

Cytology

Description

UAS regulatory sequences drive expression of a dominant negative form Egfr.

(Overton et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage 15, with Scer\GAL4^prd.PU

(Wang et al., 2017)

visible | adult stage, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Phenotype Manifest In

embryonic/larval oenocyte | embryonic stage 15, with Scer\GAL4^prd.PU

(Wang et al., 2017)

wing vein | absent, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Detailed Description

Statement

Reference

The expression of Egfr^{DN.cUa.Scer\UAS} under the control of Scer\GAL4^prd.PU eliminates oenocyte specification in Prd-positive abdominal segments during stage 15 of embryogenesis, as compared to controls.

(Wang et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Egfr^DN.cUa.UAS, Scer\GAL4^NP7397, Scer\GAL80^Su(H).GBE is a suppressor of increased occurrence of cell division | adult stage phenotype of CG12744^VDRC.cUa, Scer\GAL4^NP7397, Scer\GAL80^Su(H).GBE

(Wang et al., 2024)

Egfr^DN.cUa.UAS, Scer\GAL4^NP7397, Scer\GAL80^Su(H).GBE is a suppressor of abnormal cell number | adult stage phenotype of CG12744^VDRC.cUa, Scer\GAL4^NP7397, Scer\GAL80^Su(H).GBE

(Wang et al., 2024)

Egfr^DN.cUa.UAS, Scer\GAL4^prd.PU is a suppressor of increased cell number | embryonic stage 15 phenotype of Scer\GAL4^prd.PU, pnt^P1.UAS

(Wang et al., 2017)

NOT Suppressor of

Statement

Reference

Egfr^DN.cUa.UAS, Scer\GAL4^dpp.blk1 is a non-suppressor of neoplasia phenotype of Hipk^UAS.Tag:HA, Scer\GAL4^dpp.blk1

(Blaquiere et al., 2018)

Egfr^DN.cUa.UAS, Scer\GAL4^dpp.blk1 is a non-suppressor of abnormal cell migration | larval stage phenotype of Hipk^UAS.Tag:HA, Scer\GAL4^dpp.blk1

(Blaquiere et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Egfr^DN.cUa.UAS, Scer\GAL4^salm.EPv has wing vein | absent phenotype, suppressible by Ras85D^{V12.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Egfr^DN.cUa.UAS, Scer\GAL4^salm.EPv has wing vein | absent phenotype, suppressible by Ras64B^{V14.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Suppressor of

Statement

Reference

Egfr^DN.cUa.UAS, Scer\GAL4^NP7397, Scer\GAL80^Su(H).GBE is a suppressor of adult midgut epithelial cell phenotype of CG12744^VDRC.cUa, Scer\GAL4^NP7397, Scer\GAL80^Su(H).GBE

(Wang et al., 2024)

Egfr^DN.cUa.UAS, Scer\GAL4^prd.PU is a suppressor of embryonic/larval oenocyte | embryonic stage 15 phenotype of Scer\GAL4^prd.PU, pnt^P1.UAS

(Wang et al., 2017)

Egfr^DN.cUa.UAS, Scer\GAL4^prd.PU is a suppressor of embryonic/larval oenocyte | ectopic | embryonic stage 15 phenotype of Scer\GAL4^prd.PU, pnt^P1.UAS

(Wang et al., 2017)

NOT Suppressor of

Statement

Reference

Egfr^DN.cUa.UAS, Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of Hipk^UAS.Tag:HA, Scer\GAL4^dpp.blk1

(Blaquiere et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Egfr^{DN.cUa.Scer\UAS} under the control of Scer\GAL4^prd.PU completely suppresses the specification of oenocytes in Prd-positive abdominal segments during stage 15 of embryogenesis, even upon co-expression of pnt^P1.Scer\UAS, which when expressed alone induces increased/ectopic oenocytes in Prd-positive segments.

(Wang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Egfr^{DN.cUa.Scer\UAS}

Egfr^DN.cUa.UAS

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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