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General Information
Symbol
Dmel\mt:CoIR301S
Species
D. melanogaster
Name
FlyBase ID
FBal0219148
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: R301S.

The R301S amino acid replacement eliminates an XhoI restriction enzyme site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mt:CoIR301S mutants exhibit a wild range of defects, including growth retardation, neurodegeneration, muscular atrophy, male sterility, and reduced life span. The mutant has an extended larval phase (about 10 days at 25[o]C), and the mechanosensory bristles on the thorax are missing or thinned and shortened. Female flies are fertile but produce only 20% as many progeny as wild-type.

mt:CoIR301S flies exhibit about half the normal cytochrome c oxidase activity and significantly reduced ATP levels.

Young mt:CoIR301S mutant flies exhibit a full complement of ommatidia components, although some rhabdomeres have slight morphogenetic defects. However, the ommatidia of aged mt:CoIR301S, but not wild-type flies, are disorganized and the rhabdomeres are shrunken or completely lost, indicating age-dependent degeneration of photoreceptor neurons.

Transmission electron microscopy of flight muscle in wild-type and young mt:CoIR301S flies reveals orderly muscle fibers and fused mitochondria with long and tubular cristae. However, two weeks after adult eclosion, the mt:CoIR301S mitochondria are small and fragmented, containing many vesicular structures, and do not completely fill the intermyofibril space.

When tested in a climbing assay, mt:CoIR301S flies show mobility defects enhanced by age, consistent with age-dependent neurodegeneration and myopathy. Beyond these age-dependent neurological and muscular dysfunctions, mt:CoIR301S flies have substantially reduced longevity.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The eye ablation phenotype caused by expression of Xvas\XhoIScer\UAS.P\T.T:Mito-kdn,T:Hsap\MYC under the control of Scer\GAL4ey.PB is completely suppressed if the animals also carry mt:CoIR301S.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)