Allele Dmel\Gαq^{dsRNA.Scer\UAS.1f1}

General Information
Symbol	Dmel\Gαq^{dsRNA.Scer\UAS.1f1}	Species	D. melanogaster
Name		FlyBase ID	FBal0219376
Feature type	allele	Associated gene	Dmel\Gαq

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Banerjee et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS sequences govern expression an inverted repeat of 770bp of Gα49B cDNA sequence from a region common to all Gα49B splice variants. This sequence was amplified from of the Gα49B3α isoform cDNA using PCR primers: (5'-GGTACC(538)TCACGATACTAGCAGCATCCC-3') (5'-GGATCC(1314)CGGTGTAAGCGAGCGAAG-3') for the forward sequence and (5'-TCTAGA(539)GTGCTATGATCGTCGTAGGGA-3') (5'-GGATCC(1308)TTCGCTCGCTTCTCAATTCT-3') for the inverted sequence. A 12 nucleotide linker separates the forward and reverse sequences. (Banerjee et al., 2006)
Carried in construct	P{UAS-Gα49B.dsRNA.1f1} (Banerjee et al., 2006, Kain et al., 2008, Xia and Tully, 2007, Yao and Carlson, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	flightless \| partially, with Scer\GAL4^Ddc.PL (Banerjee et al., 2006) neurophysiology defective, with Scer\GAL4^Gα49B.PS (Kain et al., 2008) neurophysiology defective, with Scer\GAL4^{Orco.2.642.T:Hsim\VP22} (Kain et al., 2008) sensory perception defective, with Scer\GAL4^Gr21a.9.323 (Yao and Carlson, 2010) smell perception defective \| heat sensitive, with Scer\GAL80^ts.αTub84B (Xia and Tully, 2007)
Phenotype Manifest In
	adult olfactory receptor neuron Gr21a/63a, with Scer\GAL4^Gr21a.9.323 (Yao and Carlson, 2010) adult olfactory receptor neuron Or59b, with Scer\GAL4^Gα49B.PS (Kain et al., 2008)
Detailed Description
	Statement Reference Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Orco.T:Hsim\VP16] does not reduce the odour response of the ab1A or ab2A olfactory receptor neurons (tested with ethyl acetate) or of the ab3A ORN (tested with methyl butyrate). Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gr21a.9.323] results in a significant decrease in the response of the ab1C ORN to CO[[2]], across a wide response range. Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gr21a.9.323] selectively in adulthood (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele to block expression at earlier stages of development) is sufficient to decrease the response to CO[[2]] compared to wild type, at all concentrations of CO[[2]] tested. (Yao and Carlson, 2010) The electroantennograms of animals expressing Gα49B[dsRNA.Scer\UAS.1f1] under the control of either Scer\GAL4[Gα49B.PS] or Scer\GAL4[Or83b.2.642.T:Hsim\VP22] have a reduced amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate). The response to ethyl acetate of ab2 large basiconic hairs in animals expressing Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gα49B.PS] is significantly different from that of control cells; the firing frequency of the mutant ab2a neuron in response to ethyl acetate is strongly reduced compared to controls. (Kain et al., 2008) Spontaneous odor identity discrimination is significantly disrupted in transgenic Scer\GAL4[c309]/+; Galpha49B[dsRNA.Scer\UAS.1f1], Scer\GAL80[ts.αTub84B]/+ flies at the restrictive (30[o]C) but not the permissive temperature. (Xia and Tully, 2007) 60% of Gα49B[dsRNA.Scer\UAS.1f1]; Scer\GAL4[Ddc.PL] flies are flightless. However, the wing posture of these flies and the spontaneous firing rate in dorsal longitudinal flight muscle is normal. (Banerjee et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Gαq^{dsRNA.Scer\UAS.1f1}, Scer\GAL4^{Orco.2.642.T:Hsim\VP22} is a suppressor of neurophysiology defective phenotype of Scer\GAL4^{Orco.2.642.T:Hsim\VP22}, rdgA^GD1518 (Kain et al., 2008) Gαq^{dsRNA.Scer\UAS.1f1}/Scer\GAL4^Gα49B.PS is a suppressor of neurophysiology defective phenotype of rdgA¹ (Kain et al., 2008) Gαq^{dsRNA.Scer\UAS.1f1}/Scer\GAL4^Gα49B.PS is a suppressor of neurophysiology defective phenotype of rdgA³ (Kain et al., 2008)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The electroantennograms of animals co-expressing Plc21C[GD11359] and Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Or83b.2.642.T:Hsim\VP22] show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate). Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gα49B.PS] in rdgA[1] or rdgA[3] homozygotes significantly rescues the electroantennogram responses of the homozygotes. Co-expression of Gα49B[dsRNA.Scer\UAS.1f1] reduces the decrease in the amplitude of the electroantennogram in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate) that is seen in animals expressing rdgA[GD1518] under the control of Scer\GAL4[Or83b.2.642.T:Hsim\VP22]. (Kain et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Gαq^{dsRNA.Scer\UAS.1f1} is rescued by Scer\GAL4^Gr21a.9.323/Gαq^Scer\UAS.cRa (Yao and Carlson, 2010)
Comments	Co-expression of Gα49B[Scer\UAS.cRa] restores a normal response of the ab1C olfactory receptor neuron to CO[[2]] in animals expressing Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gr21a.9.323]. (Yao and Carlson, 2010)
Stocks ( 1 )
Bloomington	30735 w^*; P{UAS-Gα49B.dsRNA.1f1}2/CyO, P{ActGFP}JMR1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Gα49B^{dsRNA.Scer\UAS.1f1} Gαq^{dsRNA.Scer\UAS.1f1}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Yao and Carlson, 2010, J. Neurosci. 30(13): 4562--4572 Role of G-proteins in odor-sensing and CO2-sensing neurons in Drosophila. [FBrf0210441] Kain et al., 2008, J. Neurosci. 28(18): 4745--4755 Reduced odor responses from antennal neurons of G(q)alpha, phospholipase Cbeta, and rdgA mutants in Drosophila support a role for a phospholipid intermediate in insect olfactory transduction. [FBrf0204625] Xia and Tully, 2007, PLoS Biol. 5(10): e264 Segregation of odor identity and intensity during odor discrimination in drosophila mushroom body. [FBrf0202823] Banerjee et al., 2006, J. Neurosci. 26(32): 8278--8288 Compensation of inositol 1,4,5-trisphosphate receptor function by altering sarco-endoplasmic reticulum calcium ATPase activity in the Drosophila flight circuit. [FBrf0194925]

Allele Dmel\GαqdsRNA.Scer\UAS.1f1

Allele Dmel\Gαq^{dsRNA.Scer\UAS.1f1}