|Feature type||allele||Associated gene||Dmel\Gαq|
|Mutagen||in vitro construct - RNAi|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Scer\UAS sequences govern expression an inverted repeat of 770bp of Gα49B cDNA sequence from a region common to all Gα49B splice variants. This sequence was amplified from of the Gα49B3α isoform cDNA using PCR primers: (5'-GGTACC(538)TCACGATACTAGCAGCATCCC-3') (5'-GGATCC(1314)CGGTGTAAGCGAGCGAAG-3') for the forward sequence and (5'-TCTAGA(539)GTGCTATGATCGTCGTAGGGA-3') (5'-GGATCC(1308)TTCGCTCGCTTCTCAATTCT-3') for the inverted sequence. A 12 nucleotide linker separates the forward and reverse sequences.
|Carried in construct|
|Phenotype Manifest In|
Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Orco.T:Hsim\VP16] does not reduce the odour response of the ab1A or ab2A olfactory receptor neurons (tested with ethyl acetate) or of the ab3A ORN (tested with methyl butyrate). Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gr21a.9.323] results in a significant decrease in the response of the ab1C ORN to CO[], across a wide response range. Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gr21a.9.323] selectively in adulthood (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele to block expression at earlier stages of development) is sufficient to decrease the response to CO[] compared to wild type, at all concentrations of CO[] tested.
The electroantennograms of animals expressing Gα49B[dsRNA.Scer\UAS.1f1] under the control of either Scer\GAL4[Gα49B.PS] or Scer\GAL4[Or83b.2.642.T:Hsim\VP22] have a reduced amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate). The response to ethyl acetate of ab2 large basiconic hairs in animals expressing Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gα49B.PS] is significantly different from that of control cells; the firing frequency of the mutant ab2a neuron in response to ethyl acetate is strongly reduced compared to controls.
Spontaneous odor identity discrimination is significantly disrupted in transgenic Scer\GAL4[c309]/+; Galpha49B[dsRNA.Scer\UAS.1f1], Scer\GAL80[ts.αTub84B]/+ flies at the restrictive (30[o]C) but not the permissive temperature.
GαqdsRNA.Scer\UAS.1f1, Scer\GAL4Orco.2.642.T:Hsim\VP22 is a suppressor of neurophysiology defective phenotype of Scer\GAL4Orco.2.642.T:Hsim\VP22, rdgAGD1518
GαqdsRNA.Scer\UAS.1f1/Scer\GAL4Gα49B.PS is a suppressor of neurophysiology defective phenotype of rdgA1
|Phenotype Manifest In|
The electroantennograms of animals co-expressing Plc21C[GD11359] and Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Or83b.2.642.T:Hsim\VP22] show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate). Expression of Gα49B[dsRNA.Scer\UAS.1f1] under the control of Scer\GAL4[Gα49B.PS] in rdgA or rdgA homozygotes significantly rescues the electroantennogram responses of the homozygotes. Co-expression of Gα49B[dsRNA.Scer\UAS.1f1] reduces the decrease in the amplitude of the electroantennogram in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate) that is seen in animals expressing rdgA[GD1518] under the control of Scer\GAL4[Or83b.2.642.T:Hsim\VP22].
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 4 )|