W581term | Cap-H2-PD; W772term | Cap-H2-PE; W759term | Cap-H2-PF; W771term | Cap-H2-PG
The location of the TGG to TGA nonsense mutation was determined from the published surrounding sequences. A polymorphism in thefirst intron was also found in the strain.
gluk08819/+ ; Cap-H2Z3-0019/+ double heterozygotes show some unpairing of polytene chromosomes in nurse cells. Unpairing is significantly increased if the animals are also heterozygous for either slmbUU11 or slmb3A1.
While 30.4% of anaphase I figures from Cap-H2Z3-0019/Cap-H2TH1 males are bridged, bridging is found within only 10.8% of anaphase I figures in a heterozygous tefZ5549/tefZ5864 background. In squashed preparations anaphase I bridging is decreased from 40.5% in Cap-H2Z3-0019/Cap-H2TH1 males to 25.6% in a heterozygous tefZ5549/tefZ5864 background. The percent of anaphase I figures where homozygous chromosomes appear to be bridged decreases from 21.4 in Cap-H2Z3-0019/Cap-H2TH1 mutants to 9.3% in a heterozygous tefZ5549/tefZ5864 background. Fourth chromosomes-to-heterolog threads are greatly suppressed in a heterozygous tefZ5549/tefZ5864 background.
The persistence of polyteny that is seen in the nurse cells of stage 10 egg chambers of Cap-D3EY00456/Df(2L)Exel7023 females is enhanced by Cap-H2Z3-0019/+; the penetrance of the phenotype is enhanced from 91.3 +/- 2.3% to 100%.
77.3 +/- 9.1% of the nurse cells in stage 10 egg chambers of gluk08819/+ ; Cap-H2Z3-0019/+ double heterozygous females show persistence of polyteny, with the chromosomes having a loosened, but clear, polytene morphology.