FlyBase Allele Report: Dmel\Cap-H2[Z3-0019]

General Information

Symbol

Dmel\Cap-H2^Z3-0019

Species

D. melanogaster

Name

FlyBase ID

FBal0219642

Feature type

allele

Associated gene

Dmel\Cap-H2

Associated Insertion(s)

Carried in Construct

Also Known As

Cap-H2⁰⁰¹⁹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hartl et al., 2008)

Progenitor genotype

Cytology

Description

A to T change in the first intron and a G to A mutation that changes a Trp residue to a stop codon.

(Hartl et al., 2008)

Nucleotide substitution: A?T.

(Hartl et al., 2008)

Amino acid replacement: W?term.

(Hartl et al., 2008)

Nucleotide substitution: G?A.

(Hartl et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:10,765,260..10,765,260 [+]

Nucleotide change:

G10765260A

Reported nucleotide change:

G?A

Amino acid change:

W581term | Cap-H2-PD; W772term | Cap-H2-PE; W759term | Cap-H2-PF; W771term | Cap-H2-PG

Reported amino acid change:

W?term

Comment:

The location of the TGG to TGA nonsense mutation was determined from the published surrounding sequences. A polymorphism in thefirst intron was also found in the strain.

(Hartl et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Hartl et al., 2008)

abnormal meiotic cell cycle (with Cap-H2^Z3-5163)

(Hartl et al., 2008)

male semi-fertile (with Cap-H2^Z3-5163)

(Hartl et al., 2008)

male sterile (with Cap-H2^TH1)

(Hartl et al., 2008)

male sterile (with Df(3R)Exel6159)

(Hartl et al., 2008)

Phenotype Manifest In

polytene chromosome & nurse cell (with Cap-H2^Z3-5163)

(Hartl et al., 2008)

polytene chromosome & nurse cell (with Df(3R)Exel6159)

(Hartl et al., 2008)

primary spermatocyte in meiotic anaphase I (with Cap-H2^TH1)

(Hartl et al., 2008)

primary spermatocyte in meiotic prophase I (with Cap-H2^TH1)

(Hartl et al., 2008)

spermatozoon

(Hartl et al., 2008)

Detailed Description

Statement

Reference

Cap-H2^Z3-0019/Df(3R)Exel6159 and Cap-H2^Z3-0019/Cap-H2^TH1 transheterozygous males are sterile.

Cap-H2^Z3-0019 homozygous males are seemingly devoid of sperm, indicating a defect in gamete production.

Cap-H2^Z3-0019/Cap-H2^Z3-5163 mutants exhibit decreased fertility. There is no significant difference in male fertility between Cap-H2^Z3-0019/Cap-H2^Z3-5163 and Cap-H2^Z3-5163/+ males.

Sex chromosome nondisjunction is not found in Cap-H2^Z3-0019, Cap-H2^Z3-0019/Cap-H2^Z3-5163 or Cap-H2^Z3-0019/+ mutants.

Fourth chromosome segregation does not differ substantially between Cap-H2^Z3-0019/Cap-H2^Z3-5163 and Cap-H2^Z3-0019 heterozygous control males.

Cap-H2^Z3-0019/Cap-H2^Z3-5163 mutants exhibit an elevated level of exceptional progeny where the second or third chromosomes are exceptional. in both cases the exceptional class most over represented are those from fertilization events involving sperm that lack a 2nd or 3rd chromosome.

Cap-H2^Z3-0019/Cap-H2^Z3-5163 and Cap-H2^Z3-0019/+ mutants exhibit meiosis I nondisjunction. There may also be a slight increase in meiosis II nondisjunction.

In male sterile mutants of the genotype Cap-H2^Z3-0019/Cap-H2^TH1, chromosomal organizational steps throughout prophase I are defective, as normal territory formation is never observed in 100% of S2, S4, and S6 stages. Instead, chromatin is seemingly dispersed within the nucleus.

No prophase I defects are observed in Cap-H2^Z3-0019/Cap-H2^Z3-5163 males, although subtle morphological changes may be difficult to detect.

M1 of meiosis I may be morphologically abnormal in Cap-H2^Z3-0019/Cap-H2^TH1 mutants. Despite not forming normal chromosome territories and possibly never reaching normal M1 chromosomal structure, there are no unusual features detected in Cap-H2^Z3-0019/Cap-H2^TH1 male sterile metaphase I figures. Anaphase I is clearly not normal in Cap-H2^Z3-0019/Cap-H2^TH1 mutants, where bridges are often found between segregating sets of chromosomes. The frequency of these bridges occurs in a manner tha matches other phenotypic trends, found in 30.4% of the anaphase I figures for sterile Cap-H2^Z3-0019/Cap-H2^TH1 males, 11.5% for Cap-H2^Z3-0019/Cap-H2^TH1 males that are fertile yet undergo 2nd and 3rd chromosome loss, and never in the wild-type. Whereas bridging anaphase I figures are never observed in wild-type squashed chromosome preparations, bridging occurs in 40.5% of those from Cap-H2^Z3-0019/Cap-H2^TH1 mutant males. Of the total anaphase I figures from Cap-H2^Z3-0019/Cap-H2^TH1 mutant testes, 21.4% appear to have anaphase I bridging between homologous chromosomes. The 4th chromosome is bridged in 4.8% of anaphase I figures. As an expected outcome of cosegregation in meiosis I, aneuploidy in prophase II and anaphase II figures is observed.

In onion stages from Cap-H2^Z3-0019 homozygotes, micronuclei are often observed which may be the manifestation of chromatin lost through anaphase I bridging.

(Hartl et al., 2008)

Homozygous nurse cells do not exhibit an alteration in endocycling compared to controls; there is no significant difference in the number of homozygous and heterozygous nurse cells undergoing S phase per stage 5, 6, 10 and 11/12 egg chambers. Homozygous nurse cells do not show a change in DNA replication content compared to controls.

Homozygous larval neuroblasts do not show a change in cell cycle regulation compared to controls (assayed using flow cytometry analysis).

(Hartl et al., 2008)

Polyteny persists in 100% of the nurse cells in stage 10 egg chambers of Cap-H2^Z3-0019/Cap-H2^Z3-5163 and Cap-H2^Z3-0019/Df(3R)Exel6159 females (in contrast to wild-type nurse cells, which disassemble their polytene chromosomes during mid-oogenesis).

(Hartl et al., 2008)

Cap-H2^Z3-0019 has no dominant effect on position effect variegation at the w locus caused by In(1)w^m4h.

(Sweeney et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cap-H2^Z3-5163/Cap-H2^Z3-0019 has male semi-fertile phenotype, enhanceable by glu[+]/glu^k08819

(Hartl et al., 2008)

Enhancer of

Statement

Reference

Cap-H2^Z3-0019/Cap-H2[+] is an enhancer of visible | dominant phenotype of Ubx^1:Cbx-1

(Hartl et al., 2008)

NOT Enhancer of

Statement

Reference

Cap-H2^Z3-0019/Cap-H2[+] is a non-enhancer of visible | adult stage phenotype of eya^E4/eya²

(Tian et al., 2019)

Cap-H2^Z3-0019/Cap-H2[+] is a non-enhancer of abnormal size | adult stage phenotype of eya^E4/eya²

(Tian et al., 2019)

Suppressor of

Statement

Reference

Cap-H2^Z3-5163/Cap-H2^Z3-0019 is a suppressor | partially of visible | adult stage phenotype of eya^E4/eya²

(Tian et al., 2019)

Cap-H2^Z3-5163/Cap-H2^Z3-0019 is a suppressor | partially of abnormal size | adult stage phenotype of eya^E4/eya²

(Tian et al., 2019)

Df(3R)Exel6159/Cap-H2^Z3-0019 is a suppressor | partially of abnormal body color phenotype of y^82f29/y^1#8

(Hartl et al., 2008)

NOT Suppressor of

Statement

Reference

Cap-H2^Z3-0019/Cap-H2[+] is a non-suppressor of visible | adult stage phenotype of eya^E4/eya²

(Tian et al., 2019)

Cap-H2^Z3-0019/Cap-H2[+] is a non-suppressor of abnormal size | adult stage phenotype of eya^E4/eya²

(Tian et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome phenotype, enhanceable by slmb[+]/slmb^UU11

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has nurse cell phenotype, enhanceable by slmb[+]/slmb^UU11

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome phenotype, enhanceable by slmb^3A1/slmb[+]

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has nurse cell phenotype, enhanceable by slmb^3A1/slmb[+]

(Buster et al., 2013)

Suppressed by

Statement

Reference

Cap-H2^Z3-0019/Cap-H2^TH1 has primary spermatocyte in meiotic anaphase I phenotype, suppressible by tef^Z5549/tef^Z5864

(Hartl et al., 2008)

Enhancer of

Statement

Reference

Cap-H2^Z3-0019/Cap-H2[+] is an enhancer of wing phenotype of Ubx^1:Cbx-1

(Hartl et al., 2008)

Cap-H2^Z3-0019/Cap-H2[+] is an enhancer of polytene chromosome & nurse cell phenotype of Cap-D3^EY00456/Df(2L)Exel7023

(Hartl et al., 2008)

NOT Enhancer of

Statement

Reference

Cap-H2^Z3-0019/Cap-H2[+] is a non-enhancer of eye phenotype of eya^E4/eya²

(Tian et al., 2019)

Suppressor of

Statement

Reference

Cap-H2^Z3-5163/Cap-H2^Z3-0019 is a suppressor | partially of eye phenotype of eya^E4/eya²

(Tian et al., 2019)

Df(3R)Exel6159/Cap-H2^Z3-0019 is a suppressor | partially of adult cuticle phenotype of y^82f29/y^1#8

(Hartl et al., 2008)

NOT Suppressor of

Statement

Reference

Cap-H2^Z3-0019/Cap-H2[+] is a non-suppressor of eye phenotype of eya^E4/eya²

(Tian et al., 2019)

Other

Statement

Reference

Cap-H2^Z3-0019, glu[+]/glu^k08819 has nurse cell phenotype

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome phenotype

(Buster et al., 2013)

Cap-H2^Z3-0019, glu[+]/glu^k08819 has polytene chromosome & nurse cell phenotype

(Hartl et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

glu^k08819/+ ; Cap-H2^Z3-0019/+ double heterozygotes show some unpairing of polytene chromosomes in nurse cells. Unpairing is significantly increased if the animals are also heterozygous for either slmb^UU11 or slmb^3A1.

(Buster et al., 2013)

A glu^k08819 heterozygous background lead to a substantial decrease in fertility for Cap-H2^Z3-5163/Cap-H2^Z3-0019 mutants.

While 30.4% of anaphase I figures from Cap-H2^Z3-0019/Cap-H2^TH1 males are bridged, bridging is found within only 10.8% of anaphase I figures in a heterozygous tef^Z5549/tef^Z5864 background. In squashed preparations anaphase I bridging is decreased from 40.5% in Cap-H2^Z3-0019/Cap-H2^TH1 males to 25.6% in a heterozygous tef^Z5549/tef^Z5864 background. The percent of anaphase I figures where homozygous chromosomes appear to be bridged decreases from 21.4 in Cap-H2^Z3-0019/Cap-H2^TH1 mutants to 9.3% in a heterozygous tef^Z5549/tef^Z5864 background. Fourth chromosomes-to-heterolog threads are greatly suppressed in a heterozygous tef^Z5549/tef^Z5864 background.

(Hartl et al., 2008)

The severity of the Ubx^1:Cbx-1/+ wing phenotype is dominantly enhanced by Cap-H2^Z3-0019; the proportion of flies that show blistering or necrosis of the wing is increased.

The severity of the T(2;3)BTD71/+ wing phenotype is dominantly enhanced by Cap-H2^Z3-0019; the proportion of flies that show withering at the posterior of the wing is increased.

(Hartl et al., 2008)

The persistence of polyteny that is seen in the nurse cells of stage 10 egg chambers of Cap-D3^EY00456/Df(2L)Exel7023 females is enhanced by Cap-H2^Z3-0019/+; the penetrance of the phenotype is enhanced from 91.3 +/- 2.3% to 100%.

77.3 +/- 9.1% of the nurse cells in stage 10 egg chambers of glu^k08819/+ ; Cap-H2^Z3-0019/+ double heterozygous females show persistence of polyteny, with the chromosomes having a loosened, but clear, polytene morphology.

Body colour: The reduced body pigmentation seen in y^1#8/y^82f29 flies is partially suppressed by Cap-H2^Z3-0019/Df(3R)Exel6159.

(Hartl et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Cap-H2^Z3-5163

(Hartl et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cap-H2⁰⁰¹⁹

(Tian et al., 2019, Wallace et al., 2015)

Cap-H2^Z3-0019

(Wallace et al., 2015, Johnston and Desplan, 2014, Buster et al., 2013, Hartl et al., 2008, Hartl et al., 2008, Hartl et al., 2008)

Z3-0019

(Sweeney et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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