FlyBase Allele Report: Dmel\Atg7[d77]

General Information

Symbol

Dmel\Atg7^d77

Species

D. melanogaster

Name

FlyBase ID

FBal0219652

Feature type

allele

Associated gene

Dmel\Atg7

Associated Insertion(s)

Carried in Construct

Also Known As

Atg7^Δ77, Atg7[d77]

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Juhász et al., 2007)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{XP}Atg7^d06996

(Juhász et al., 2007)

Caused by aberration

Df(2R)Atg7^d77

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the P{XP}Atg7^d06996 insertion, resulting in a deletion that removes exons 5 and 6 and most of exon 4 of Atg7. The CG5335 gene (which is within an intron of Atg7) is completely deleted.

(Juhász et al., 2007)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | progressive (with Atg7^d14)

(Gupta et al., 2013)

abnormal neuroanatomy (with Atg7^d14)

(Issman-Zecharya and Schuldiner, 2014)

abnormal size | adult stage (with Atg7^d14)

(Nagy et al., 2017)

abnormal stress response (with Atg7^d14)

(Juhász et al., 2007)

chemical sensitive (with Atg7^d14)

(Juhász et al., 2007)

decreased cell death | adult stage | nutrition conditional (with Atg7^d14)

(Hou et al., 2008)

decreased cell death | prepupal stage (with Atg7^d14)

(Juhász et al., 2007)

decreased cell number | dominant | larval stage

(Sim et al., 2019)

increased cell death | adult stage (with Atg7^d14)

(Juhász et al., 2007)

short lived (with Atg7^d14)

(Wen et al., 2017, Juhász et al., 2007)

viable

(Juhász et al., 2007)

viable (with Atg7^d14)

(Barth et al., 2011)

Phenotype Manifest In

adult brain (with Atg7^d14)

(Juhász et al., 2007)

adult gut (with Atg7^d14)

(Nagy et al., 2017)

autophagic vacuole & larval midgut (with Atg7^d14)

(Juhász et al., 2007)

egg chamber | nutrition conditional (with Atg7^d14)

(Hou et al., 2008)

embryonic/larval fat body | third instar larval stage (with Atg7^d14)

(Pircs et al., 2012)

embryonic/larval midgut | prepupal stage (with Atg7^d14)

(Juhász et al., 2007)

female germline cyst | nutrition conditional (with Atg7^d14)

(Hou et al., 2008)

gamma Kenyon cell (with Atg7^d14)

(Issman-Zecharya and Schuldiner, 2014)

germarium region 2 | nutrition conditional (with Atg7^d14)

(Hou et al., 2008)

major mitochondrial derivative (with Atg7^d14)

(Politi et al., 2014)

rhabdomere

(Wang et al., 2009)

RP2 motor neuron | larval stage

(Sim et al., 2019)

Detailed Description

Statement

Reference

Atg7^d77 heterozygous larvae exhibit a mild decrease in the number of RP2 motor neurons, compared to controls.

(Sim et al., 2019)

Atg7^d14/Atg7^d77 transheterozygous adults exhibit significant decreases in gut diameter and area, but not in gut length, as compared to controls.

(Nagy et al., 2017)

Atg7^d14/Atg7^d77 transheterozygotes are short lived compared to controls.

(Wen et al., 2017)

Mushroom body gamma neurons in Atg7^d14/Atg7^d77 animals show a very mild pruning defect.

(Issman-Zecharya and Schuldiner, 2014)

Embryos derived from Atg7^d14/Atg7^d77 females develop with normal kinetics at least until the end of cellularisation and the beginning of gastrulation. The mutant eggs show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type. Early fertilised eggs have multivesicular clusters which are largely devoid of microvesicles.

(Politi et al., 2014)

Compared with wild-type, Atg7^d14/Atg7^d77 flies show reduce memory scores at a young age (3 days of age), which further declines relative to controls at later time points (20 days of age). Wild-type flies show a decline in both anesthesia-sensitive and olfactory short-term memory, both of which can be ameliorated by spermidine-feeding. Notably, the olfactory short term memory-protective effects of spermidine-administration are eliminated in the mutants.

(Gupta et al., 2013)

Atg7^d77/Atg7^d14 mutants display basal autophagy defects in larval fat body cells.

(Pircs et al., 2012)

Flies lacking Atg7^d77/Atg7^d14 are viable, but unable to induce autophagy.

Eggs derived from Atg7^d77 homozygous mutants show a slight reduction in hatching rates compared with heterozygous controls, with 18% of the eggs displaying eggshell defects, suggesting that Atg7 mutations have only minor effects on egg development.

(Barth et al., 2011)

While all seven rhabdomeres are detected in the ommatidia of young (<2 day old) Atg7^d77 flies, after 20 days of light/dark cycling, few rhabdomeres are found.

(Wang et al., 2009)

Nutrient-deprived Atg7^d77/Atg7^d14 mutant flies display reduced levels of autophagy (punctate Lysotracker staining) in region two of the germarium and in stage eight degenerating egg chambers compared to controls.

Fewer apoptotic (TUNEL positive) cells are seen in region two cysts in nutrient-deprived Atg7^d77/Atg7^d14 mutant flies than are seen in wild type. Degenerating stage eight egg chambers in starved Atg7^d77/Atg7^d14 mutants show low or no TUNEL-positive staining compared with controls, although nuclear DNA condensation is still observed in these egg chambers.

(Hou et al., 2008)

Atg7^d14/Atg7^d77 fat body cells show a strong reduction of autophagosomes and autolysosomes following 3 hours of starvation compared to control cells.

Midgut cells of Atg7^d14/Atg7^d77 larvae show a strong reduction of autophagosomes and autolysosomes compared to control larvae, at the wandering stage, just prior to pupariation. No major defects are seen in elimination of the larval midgut or in adult midgut epithelium formation in Atg7^d14/Atg7^d77 animals during metamorphosis. However, DNA fragmentation indicative of cell death is greatly reduced in the mutant midgut at the time of pupariation (DNA fragmentation is readily detected at this stage in control midguts) and there is an approximately 4 hour increase in the average time required to complete metamorphosis in the mutant animals.

Under conditions of complete starvation or a sugar-only diet, Atg7^d14/Atg7^d77 mutants show an accelerated lethality compared to control flies. The mutant flies also show a reduced life span compared to controls under normal environmental conditions.

Atg7^d14/Atg7^d77 mutants are hypersensitive to treatment with paraquat compared to controls.

Atg7^d14/Atg7^d77 flies show a significant age-dependent decline in climbing performance. The brains of mutant adults contain ubiquitin-positive inclusion bodies in the neurons, with these structures being seen in 3 day old adults, and increasing in number with age. The brains of 30 day old mutant flies contain dead neurons in addition to the inclusion bodies, show moderate vacuolization and most brain cells show extensive DNA fragmentation (assayed using TUNEL labeling).

(Juhász et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atg7^d77 has decreased cell number | dominant | larval stage phenotype, enhanceable by Df(2L)cl7/bchs^58M

(Sim et al., 2019)

Suppressed by

Statement

Reference

Atg7^d14/Atg7^d77, Pink1^B9 has visible | adult stage phenotype, suppressible by Hsap\DNM1L^{UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has visible | adult stage phenotype, suppressible by Hsap\DNM1L^{S616D.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has visible | adult stage phenotype, suppressible | partially by Hsap\DNM1L^{S616A.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Enhancer of

Statement

Reference

Atg7[+]/Atg7^d77 is an enhancer of abnormal neuroanatomy | larval stage phenotype of Df(2L)cl7/bchs^58M

(Sim et al., 2019)

Atg7[+]/Atg7^d77 is an enhancer of decreased cell number | larval stage phenotype of Df(2L)cl7/bchs^58M

(Sim et al., 2019)

NOT Enhancer of

Statement

Reference

Atg7[+]/Atg7^d77 is a non-enhancer of decreased cell number | larval stage phenotype of bchs^17M/Df(2L)cl7

(Sim et al., 2019)

NOT Suppressor of

Statement

Reference

Atg7^d14/Atg7^d77 is a non-suppressor of visible | adult stage phenotype of Pink1^B9

(Han et al., 2020)

Atg7[+]/Atg7^d77 is a non-suppressor of decreased cell number | larval stage phenotype of bchs^17M/Df(2L)cl7

(Sim et al., 2019)

Atg7^d77 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Atg7^d77 is a non-suppressor of decreased cell number | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Atg7^d77 has RP2 motor neuron | larval stage phenotype, enhanceable by Df(2L)cl7/bchs^58M

(Sim et al., 2019)

Suppressed by

Statement

Reference

Atg7^d14/Atg7^d77, Pink1^B9 has mitochondrion | adult stage phenotype, suppressible by Hsap\DNM1L^{UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has adult thorax phenotype, suppressible | partially by Hsap\DNM1L^{S616D.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has mitochondrion | adult stage phenotype, suppressible | partially by Hsap\DNM1L^{S616D.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has adult thorax phenotype, suppressible | partially by Hsap\DNM1L^{S616A.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has adult thorax phenotype, suppressible by Hsap\DNM1L^{UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

NOT suppressed by

Statement

Reference

Atg7^d14/Atg7^d77, Pink1^B9 has adult thorax phenotype, non-suppressible | partially by Hsap\DNM1L^{S616A.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Atg7^d14/Atg7^d77, Pink1^B9 has mitochondrion | adult stage phenotype, non-suppressible by Hsap\DNM1L^{S616A.UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4^Mhc.PU

(Han et al., 2020)

Enhancer of

Statement

Reference

Atg7[+]/Atg7^d77 is an enhancer of RP2 motor neuron | larval stage phenotype of Df(2L)cl7/bchs^58M

(Sim et al., 2019)

NOT Enhancer of

Statement

Reference

Atg7^d14/Atg7^d77 is a non-enhancer of mitochondrial crista | adult stage phenotype of Pink1^B9

(Han et al., 2020)

Atg7^d14/Atg7^d77 is a non-enhancer of indirect flight muscle cell phenotype of Pink1^B9

(Han et al., 2020)

Atg7^d14/Atg7^d77 is a non-enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Han et al., 2020)

Atg7[+]/Atg7^d77 is a non-enhancer of RP2 motor neuron | larval stage phenotype of bchs^17M/Df(2L)cl7

(Sim et al., 2019)

NOT Suppressor of

Statement

Reference

Atg7^d14/Atg7^d77 is a non-suppressor of mitochondrial crista | adult stage phenotype of Pink1^B9

(Han et al., 2020)

Atg7^d14/Atg7^d77 is a non-suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Han et al., 2020)

Atg7^d14/Atg7^d77 is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Han et al., 2020)

Atg7[+]/Atg7^d77 is a non-suppressor of RP2 motor neuron | larval stage phenotype of bchs^17M/Df(2L)cl7

(Sim et al., 2019)

Atg7^d77 is a non-suppressor of neuroblast | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Other

Statement

Reference

Atg7^d14/Atg7^d77, Wdr24¹ has lysosome phenotype

(Cai et al., 2016)

Atg7^d14/Atg7^d77, Wdr24¹ has ovariole phenotype

(Cai et al., 2016)

Atg7[+]/Atg7^d77, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Wdr24¹/Wdr24¹ ;Atg7^d14/Atg7^d77 double mutant ovarioles show accumulation of late endosomes/lysosomes (not autolysosomes), without activation of autophagy.

(Cai et al., 2016)

Atg7^d77 does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

(Kuang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} under the Scer\GAL4^shot-OK307 driver in adults heterozygous for Atg7^d77 has no phenotype, unless the animals are also heterozygous for htt^98E2, in which case a severe loss of thoracic somatic muscles is observed.

(Rui et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Atg7 d77

(Han et al., 2020)

Atg7⁷⁷

(Jipa et al., 2021)

Atg7^Δ77

(Jacomin et al., 2021, Jacomin et al., 2020, Jacomin et al., 2019, Billes et al., 2018, Barth et al., 2011)

Atg7[d77]

(Nagy et al., 2015, Takáts et al., 2014, Takáts et al., 2013)

atg7^d77

(Tarayrah-Ibraheim et al., 2021, Cai et al., 2016, Rui et al., 2015, Politi et al., 2014, Wang et al., 2009)

atg7^Δ77

(Tusco et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (37)

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