FlyBase Allele Report: Dmel\Tk[JF01818]

General Information

Symbol

Dmel\Tk^JF01818

Species

D. melanogaster

Name

FlyBase ID

FBal0219827

Feature type

allele

Associated gene

Dmel\Tk

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01818}

Also Known As

Tk^RNAi, UAS-TK RNAi, UAS-Tk-RNAi, UAS-Tk^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

(Perkins et al., 2009.1.15)

Progenitor genotype

Carried in construct

P{TRiP.JF01818}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01818

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

enterocyte, with Scer\GAL4^Tk.PS

(Song et al., 2014)

oocyte | increased number, with Scer\GAL4^Lsp2.3.1

(Grmai et al., 2024)

Detailed Description

Statement

Reference

Adults expressing AcCoAS^HMS02314 under the control of Scer\GAL4^Tk.PS show increased lethality to infection with V. cholerae.

(Jugder et al., 2021)

Females expressing Tk^JF01818 under the control of Scer\GAL4^Tk.PS fail to show the expected mating-induced increase in germline stem cells in the germarium, as compared to controls.

(Ameku et al., 2018)

Expression of Tk^JF01818 under the control of Scer\GAL4^elav-C155 in adults results in a significant increase in the strength of circadian locomotor rhythms, compared to controls.

(Cavey et al., 2016)

24 hours after UV-induced nociceptive sensitization, early third instar larvae with expression of Tk^JF01818 driven by Scer\GAL4^elav-C155 (but not Scer\GAL4^ppk.1.9) show significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to controls.

(Im et al., 2015)

Knockdown of Tk through expression of Tk^JF01818 under the control of Scer\GAL4^Tkg.PS leads to an increase in intestinal lipid production, haemolymph triglyceride levels, and triglyceride storage.

Neuronal expression of Tk^JF01818, under the control of Scer\GAL4^elav.PLu results in increased locomotor activity and reduces olfactory responses to certain chemicals. In addition body weight is affected in these flies.

Tk knockdown in the gut, through expression of Tk^JF01818 under the control of Scer\GAL4^Tkg.PS does not affect locomotor activity or the olfactory response. Body weight is not affected in these flies.

Knockdown of Tk, through expression of Tk^JF01818 under the control of Scer\GAL4^Tkg.PS dramatically decreases CREB transcriptional activity in enterocytes compared to controls. Activation of PKA, through feeding the flies forskolin, a PKA agonist, restores CREB transcriptional activity.

(Song et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Tk^JF01818, Scer\GAL4^{Act5C.PS-FRT3} is a suppressor of increased cell growth rate | adult stage | somatic clone - tissue specific phenotype of Apc^GD1333, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483