FB2025_01 , released February 20, 2025
Allele: Dmel\PtenJF01859
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General Information
Symbol
Dmel\PtenJF01859
Species
D. melanogaster
Name
FlyBase ID
FBal0219868
Feature type
allele
Associated gene
Dmel\Pten
Associated Insertion(s)
Carried in Construct
P{TRiP.JF01859}
Also Known As
UAS-Pten-RNAi, UAS-pten RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Perkins et al., 2009.1.15)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Perkins et al., 2009.1.15)
Mutagen
in vitro construct
(Perkins et al., 2009.1.15)
Progenitor genotype
Carried in construct
P{TRiP.JF01859}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-JF01859
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PtenJF01859 under the control of Scer\GAL4e16A results in a significant increase in trichome density in the wing.

      (Das et al., 2014)

      Expression of PtenJF01859 in the pupal wing under the control of Scer\GAL80ts.αTub84B and Scer\GAL4en.PU causes cell rearrangement defects where newly formed junctions fail to elongate and become unstable. Compared with wild-type tissues, an excess of short junctions are observed in the mutant tissue. The excess of short junctions observed in the mutant tissue is associated with a failure of junction lengthening during cell rearrangement accompanied by junction instability.

      Expression of PtenJF01859 under the control of Scer\GAL4en.PU drastically effects the ridge organization on the adult wing cuticle.

      (Bardet et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      PtenJF01859, Scer\GAL4e16A has wing hair phenotype, suppressible by trblUAS.Tag:FLAG, Scer\GAL4e16A

      (Das et al., 2014)

      PtenJF01859, Scer\GAL4en.PU, Scer\GAL80ts.αTub84B has epithelial cell | pupal stage | heat sensitive phenotype, suppressible by Rok2

      (Bardet et al., 2013)

      PtenJF01859, Scer\GAL4en.PU, Scer\GAL80ts.αTub84B has wing | pupal stage | heat sensitive phenotype, suppressible by Rok2

      (Bardet et al., 2013)

      PtenJF01859, Scer\GAL4en.PU, Scer\GAL80ts.αTub84B has cell junction | pupal stage | heat sensitive phenotype, suppressible by Rok2

      (Bardet et al., 2013)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of trblScer\UAS.T:Zzzz\FLAG suppresses the increase in trichome density seen in the wings of flies expressing PtenJF01859 under the control of Scer\GAL4e16A. The resulting flies exhibit a significant decrease in trichome density compared with controls, as is seen in trblScer\UAS.T:Zzzz\FLAG alone.

      (Das et al., 2014)

      rok2 clones generated in PtenJF01859-expressing tissue suppresses the effect of the expression of PtenJF01859 under the control of Scer\GAL80ts.αTub84B and Scer\GAL4en.PU on cell packing, namely the excess of short junctions. Despite a slightly higher proportion of non-rearranging junctions (1.15-fold), the rearranging junctions neither produce an excess of short junctions nor fluctuate extensively, but lengthen to produce long cell junctions as observed in wild-type tissue. Furthermore, the packing and cell rearrangement dynamics in the double mutant tissue is similar to the one observed in rok2 single mutant tissue.

      (Bardet et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      PtenJF01859
      Name Synonyms
      Secondary FlyBase IDs
        References (11)