FlyBase Allele Report: Dmel\CanA1[JF01871]

General Information

Symbol

Dmel\CanA1^JF01871

Species

D. melanogaster

Name

FlyBase ID

FBal0219877

Feature type

allele

Associated gene

Dmel\CanA1

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01871}

Also Known As

UAS-CanA1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

(Perkins et al., 2009.1.15)

Progenitor genotype

Carried in construct

P{TRiP.JF01871}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01871

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Wong et al., 2014)

partially lethal - majority live, with Scer\GAL4^elav.PU

(Lee et al., 2016)

Phenotype Manifest In

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Wong et al., 2014)

larval glial cell | increased number, with Scer\GAL4^elav.PU

(Lee et al., 2016)

NMJ bouton | decreased number | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Wong et al., 2014)

Detailed Description

Statement

Reference

Expression of CanA1^JF01871 under the control of Scer\GAL4^GMR.PU does not cause any adult eye phenotype compared to controls.

Expression of CanA1^JF01871 under the control of Scer\GAL4^elav.PU results in increased mortality during development and increased number of glial cells in third instar larval brain compared to controls.

(Lee et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CanA1^JF01871, Scer\GAL4^elav.PU has partially lethal - majority live phenotype, enhanceable by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav.PU

(Lee et al., 2016)

NOT Enhanced by

Statement

Reference

CanA1^JF01871, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

NOT suppressed by

Statement

Reference

CanA1^JF01871, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by iav^hypoB-1

(Wong et al., 2014)

Enhancer of

Statement

Reference

CanA1^JF01871, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU

(Lee et al., 2016)

CanA1^JF01871, Scer\GAL4^elav.PU is an enhancer of partially lethal - majority live phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav.PU

(Lee et al., 2016)

NOT Enhancer of

Statement

Reference

Scer\GAL4^VGlut1-OK371/CanA1^JF01871 is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of iav^hypoB-1

(Wong et al., 2014)

Suppressor of

Statement

Reference

CanA1^JF01871, Scer\GAL4^repo.PU is a suppressor | partially of lethal - all die during P-stage phenotype of Scer\GAL4^repo.PU, brm^GD4507

(Tegeder et al., 2019)

Other

Statement

Reference

CanA1^JF01871, Scer\GAL4^repo.PU, brm^GD4507 has partially lethal - majority die phenotype

(Tegeder et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

CanA1^JF01871, Scer\GAL4^elav.PU has larval glial cell | third instar larval stage | increased number phenotype, non-enhanceable by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav.PU

(Lee et al., 2016)

CanA1^JF01871, Scer\GAL4^VGlut1-OK371 has NMJ bouton | third instar larval stage | decreased number phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

CanA1^JF01871, Scer\GAL4^VGlut1-OK371 has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

NOT suppressed by

Statement

Reference

CanA1^JF01871, Scer\GAL4^VGlut1-OK371 has NMJ bouton | third instar larval stage | decreased number phenotype, non-suppressible by iav^hypoB-1

(Wong et al., 2014)

CanA1^JF01871, Scer\GAL4^VGlut1-OK371 has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype, non-suppressible by iav^hypoB-1

(Wong et al., 2014)

Enhancer of

Statement

Reference

CanA1^JF01871, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU

(Lee et al., 2016)

NOT Enhancer of

Statement

Reference

CanA1^JF01871, Scer\GAL4^elav.PU is a non-enhancer of larval glial cell | third instar larval stage | increased number phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav.PU

(Lee et al., 2016)

Scer\GAL4^VGlut1-OK371/CanA1^JF01871 is a non-enhancer of NMJ bouton | third instar larval stage | decreased number phenotype of iav^hypoB-1

(Wong et al., 2014)

Scer\GAL4^VGlut1-OK371/CanA1^JF01871 is a non-enhancer of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of iav^hypoB-1

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is exacerbated further by co-expression of CanA1^JF01871 RNAi.

The increased number of glial cells in third instar larval brain observed in larvae expressing either Hsap\APP^{Aβ42.Scer\UAS} or CanA1^JF01871 under the control of Scer\GAL4^elav.PU does not increase further when Hsap\APP^{Aβ42.Scer\UAS} and CanA1^JF01871 are co-expressed.

The increased mortality during development characteristic for flies expressing either Hsap\APP^{Aβ42.Scer\UAS} or under the control of CanA1^JF01871 is dramatically increased if both Hsap\APP^{Aβ42.Scer\UAS} and CanA1^JF01871 are expressed simultaneously.

(Lee et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25850

y¹ v¹; P{TRiP.JF01871}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

CanA1^JF01871

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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