FlyBase Allele Report: Dmel\SERCA[JF01948]

General Information

Symbol

Dmel\SERCA^JF01948

Species

D. melanogaster

Name

FlyBase ID

FBal0219934

Feature type

allele

Associated gene

Dmel\SERCA

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01948}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

(Perkins et al., 2009.1.15)

Progenitor genotype

Carried in construct

P{TRiP.JF01948}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01948

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of heart disease

CEA

(Kuo et al., 2014)

model of Brody myopathy

CEA

(Bruels et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Martin et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^repo.PU, Scer\GAL80^ts.αTub84B

(Ng et al., 2011)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Wong et al., 2014)

lethal, with Scer\GAL4^tin.CΔ4

(Abraham and Wolf, 2013)

Phenotype Manifest In

adult heart, with Scer\GAL4^tin.CΔ4

(Abraham and Wolf, 2013)

embryonic/larval heart | P-stage, with Scer\GAL4^NP1029

(Kuo et al., 2014)

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Wong et al., 2014)

NMJ bouton | decreased number | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Wong et al., 2014)

Detailed Description

Statement

Reference

Expression of SERCA^JF01948 in the fat body under the control of Scer\GAL4^DcG results in significant hyperglycaemia.

(Ugrankar et al., 2015)

Pupae expressing Scer\GAL4^NP1029>Ca-P60A^JF01948 display slower heart rate than do wild-type controls. The electrocardiograms (ECGs) recorded for Scer\GAL4^NP1029>Ca-P60A^JF01948 and wild-type animals also differ.

(Kuo et al., 2014)

Expression of Ca-P60A^JF01948 under the control of Scer\GAL4^tin.CΔ4 throughout development is lethal. When Ca-P60A^JF01948 is expressed post-developmentally (using the TARGET system) there is a significant decrease in cardiac function; fractional shortening is significantly diminished and end diastolic dimension is increased. As in wild type, flies expressing Ca-P60A^JF01948 under the control of Scer\GAL4^tin.CΔ4 do not undergo paralysis in response to heat shock.

(Abraham and Wolf, 2013)

Expression of Ca-P60A^JF01948 under the control of Scer\GAL4^repo.PU during the adult stage under constant darkness conditions results in the flies becoming arrhythmic or weakly rhythmic after 5-6 days of expression (the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to control the timing of expression by shifting the adults to 30[o]C at the beginning of the subjective daytime on day 1 of constant darkness and Dcr-2^Scer\UAS.cDa is present to increase RNAi efficiency).

(Ng et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

SERCA^JF01948, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by iav^hypoB-1

(Wong et al., 2014)

NOT suppressed by