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General Information
Symbol
Dmel\Stat92EdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0220151
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-STAT92E RNAi, UAS-STAT92E-IR
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat of Stat92E sequences, which correspond to nucleotides 287-1418 (this fragment includes an intron) and nucleotides 986-487 in the opposite direction.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

In a tuberculosis model by abdominal injection with Mycobacterium marinum, adult flies expressing Stat92EdsRNA.Scer\UAS under the control of: Scer\GAL4crq.Unk, in combination with gal80[ts] to restrict expression to adulthood, present a significant decrease in the bacterial burden, and a significant increase in the survival, as compared to wild-type control flies; Scer\GAL4Hml.Δ, in combination with gal80[ts] to restrict expression to adulthood, present a significant increase in the hemocyte numbers at day 6, but not day 5, after infection, as compared to wild-type control flies; Scer\GAL4c564 present similar survival, as compared to wild-type control flies.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Stat92EdsRNA.Scer\UAS under the control of Scer\GAL4c564 has no significant effect on the survival of adults, upon infection or not with Mycobacterium marinum, as compared to wild-type controls.

The expression of Stat92EdsRNA.Scer\UAS under the control of Scer\GAL4crq.Unk, in combination with gal80[ts] to restrict expression to adulthood, leads to a significant decrease in the bacterial burden, and a significant increase in the survival of adults infected with Mycobacterium marinum, as compared to wild-type controls, whereas non-infected individuals do not present significant differences in survival as compared to wild-type controls; expression under the control of Scer\GAL4Hml.Δ, in combination with gal80[ts] to restrict expression to adulthood, leads to a significant increase in the hemocyte numbers at day 6, but not at day 5, after infection with Mycobacterium marinum, as compared to wild-type controls, whereas non-infected individuals do not present significant differences in hemocyte numbers as compared to wild-type controls.

Flies expressing Stat92EdsRNA.Scer\UAS show a higher bacterial clearance activity than do control flies after injection with E.coli.

Flies expressing Stat92EdsRNA.Scer\UAS (either using heat shock to drive expression from the minimal heat shock promoter in the P{UAS-Stat92E.RNAi} construct, or using a Scer\GAL4da.G32 driver to drive expression) show a lower survival rate after infection with E.coli than do control flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1KO;Cherry/Sulf1ΔP1 mutants can be suppressed by expression of Stat92EdsRNA.Scer\UAS under the control of Scer\GAL4NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).

The lower survival rate seen in flies expressing Stat92EdsRNA.Scer\UAS (either using heat shock to drive expression from the minimal heat shock promoter in the P{UAS-Stat92E.RNAi} construct, or using a Scer\GAL4da.G32 driver to drive expression) after infection with E.coli is suppressed if the flies are also heterozygous for RelE20.

Xenogenetic Interactions
Statement
Reference

The aggressive tissue overgrowth observed in third instar larval wing discs carrying somatic (flip-out) clones simultaneously expressing Snr1HMS00363 and BacA\p35Scer\UAS.cHa driven by Scer\GAL4Act.PU is significantly suppressed by co-expression of Stat92EdsRNA.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Stat92EdsRNA.Scer\UAS
Stat92EdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (8)