Allele Dmel\TrpA1^ins.3'

General Information
Symbol	Dmel\TrpA1^ins.3'	Species	D. melanogaster
Name		FlyBase ID	FBal0220311
Feature type	allele	Associated gene	Dmel\TrpA1

Allele class
Mutagen	recombination, ends-in gene targeting
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-in gene targeting (Hamada et al., 2008) recombination (Hamada et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A targeted recombination event at the TrpA1 locus which has generated a tandem array of two mutated copies of TrpA1 (represented by TrpA1[ins.5'] and TrpA1[ins.3']). One copy is deleted for the sixth transmembrane domain and carboxy terminus, whereas the other copy is deleted for the promoter region and start codon and contains a translational stop before the transmembrane domains). (Hamada et al., 2008)
Caused by aberration	Dp(3;3)TrpA1^ins (Hamada et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (with TrpA1^ins.5') (Hamada et al., 2008) behavior defective (with TrpA1^ins.5'), with Df(3L)ED4415 (Hamada et al., 2008, Hamada et al., 2008, Hamada et al., 2008) neurophysiology defective \| adult stage (with TrpA1^ins.5') (Hamada et al., 2008) taste perception defective (with TrpA1^ins.5') (Kim et al., 2010)
Phenotype Manifest In
	AC thermosensory neuron (with TrpA1^ins.5') (Hamada et al., 2008)
Detailed Description
	Statement Reference TrpA1[ins.5'], TrpA1[ins.3'] flies display a reduced aversion to aristolochic acid compared to wild type. In contrast to wild type, TrpA1[ins.5'], TrpA1[ins.3'] flies have virtually no aristolochic acid-induced action potentials in tip recordings of sensillum s6. (Kim et al., 2010) Dp(3;3)TrpA1[ins] flies (which carry TrpA1[ins.5'] and TrpA1[ins.3']) show an altered distribution along a thermal gradient compared to wild-type flies. The mutant flies show increased accumulation in the warmest regions (28-32[o]C) of the gradient, but not in the coolest region (18-22[o]C). Df(3L)ED4415/Dp(3;3)TrpA1[ins] flies show an altered distribution along a thermal gradient compared to wild-type flies. The mutant flies show increased accumulation in the warmest regions (28-32[o]C) of the gradient, but not in the coolest region (18-22[o]C). Anterior cell (AC) neurons in the brains of Dp(3;3)TrpA1[ins] flies do not show warmth-responsive increases in intracellular calcium, in contrast to control AC neurons. (Hamada et al., 2008)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Associated with: TrpA1[ins.5']. (Hamada et al., 2008)
Comments
	Generated by 'ends-in' targeting of the TrpA1 locus. (Hamada et al., 2008)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	TrpA1^ins.3' trpA1^ins (Kim et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Kim et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(18): 8440--8445 Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons. [FBrf0210738] Hamada et al., 2008, Nature 454(7201): 217--220 An internal thermal sensor controlling temperature preference in Drosophila. [FBrf0205189]

Allele Dmel\TrpA1ins.3'

Allele Dmel\TrpA1^ins.3'