FlyBase Allele Report: Dmel\sti[Z3-5829]

General Information

Symbol

Dmel\sti^Z3-5829

Species

D. melanogaster

Name

FlyBase ID

FBal0220349

Feature type

allele

Associated gene

Dmel\sti

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

heat sensitive loss of function allele

Mutagen

Nature of the Allele

Allele class

heat sensitive loss of function allele

(Sweeney et al., 2008)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: N1799I.

(Sweeney et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:12,508,526..12,508,526 [-]

Nucleotide change:

A12508526T

Amino acid change:

N1799I | sti-PA; N1803I | sti-PB

Reported amino acid change:

N1799I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sweeney et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation | recessive, with Dp(?;2)bw^D/+:

(Sweeney et al., 2008)

female sterile

(Sweeney et al., 2008)

lethal | heat sensitive (with sti³)

(Sweeney et al., 2008)

lethal | recessive | heat sensitive

(Sweeney et al., 2008)

suppressor of variegation | dominant, with In(1)w^m4h

(Sweeney et al., 2008)

suppressor of variegation | dominant, with P{lacW}50C.x6

(Sweeney et al., 2008)

viable

(Sweeney et al., 2008)

visible | heat sensitive (with sti³)

(Sweeney et al., 2008)

visible | recessive

(Sweeney et al., 2008)

Phenotype Manifest In

eye

(Sweeney et al., 2008)

eye | heat sensitive (with sti³)

(Sweeney et al., 2008)

follicle cell

(Sweeney et al., 2008)

interommatidial bristle

(Sweeney et al., 2008)

interommatidial bristle | heat sensitive (with sti³)

(Sweeney et al., 2008)

larval brain

(Sweeney et al., 2008)

nucleus & follicle cell | conditional - heat sensitive

(Sweeney et al., 2008)

nucleus & follicle cell | conditional - heat sensitive (with sti³)

(Sweeney et al., 2008)

Detailed Description

Statement

Reference

Late stage 12-13 egg chambers in homozygous females have enlarged nuclei in the follicle cells and multinucleated follicle cells are also seen. Follicle cell size and shape is disrupted and the follicle cell layer is disorganised. The DNA content of the follicle cells is increased to 32c. The fraction of multinucleated follicle cells is increased in flies reared at 21[o]C compared to those reared at 18[o]C. The nuclear diameter of stage 12-13 homozygous follicle cells is normal at 18[o], but is increased compared to wild type at 21[o]C.

Homozygous larvae have polyploid cells in the brain.

More homozygous adults are produced when cultured at 18[o]C compared to rearing at 21[o]C and no homozygous adults are recovered at 25[o]C.

The fraction of sti^Z3-5829/sti³ individuals recovered in a cross between sti^Z3-5829/TM6B and sti³/TM6B animals is significantly less than expected at 25[o]C, whereas at 18[o]C, there is no significant difference between the fraction of heterozygous and sti^Z3-5829/sti³ progeny recovered. At 18[o]C, both male and female sti^Z3-5829/sti³ progeny are equally represented, whereas at 25[o]C, male sti^Z3-5829/sti³ progeny are significantly reduced.

The nuclear diameter of stage 12-13 sti^Z3-5829/sti³ follicle cells is normal at 18[o], but is increased compared to wild type at 21[o]C.

In sti^Z3-5829 homozygotes and sti^Z3-5829/sti³ flies, 100% have missing eye bristles and 37% have rough eyes when reared at 21[o]C. At 25[o]C, 100% of sti^Z3-5829/sti³ adults have missing eye bristles and rough eyes.

(Sweeney et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sti^Z3-5829 has visible phenotype, enhanceable by AGO1^k08121/AGO1[+]

(Sweeney et al., 2008)

sti^Z3-5829 has visible phenotype, enhanceable by AGO1[+]/AGO1^k00208

(Sweeney et al., 2008)

Suppressor of

Statement

Reference

sti[+]/sti^Z3-5829 is a suppressor of visible phenotype of AGO1^UAS.cZa, Scer\GAL4^GMR.PF

(Sweeney et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

sti^Z3-5829 has eye phenotype, enhanceable by AGO1^k08121/AGO1[+]

(Sweeney et al., 2008)

sti^Z3-5829 has eye phenotype, enhanceable by AGO1[+]/AGO1^k00208

(Sweeney et al., 2008)

Suppressor of

Statement

Reference

sti[+]/sti^Z3-5829 is a suppressor of eye phenotype of AGO1^UAS.cZa, Scer\GAL4^GMR.PF

(Sweeney et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The rough and small eye phenotypes of flies expressing AGO1^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF are suppressed by sti^Z3-5829/+, with suppression consistently being stronger in females than in males.

The sti^Z3-5829 rough eye phenotype is dominantly enhanced by AGO1^k08121 and by AGO1^k00208.

(Sweeney et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sti¹

(Sweeney et al., 2008)

sti³

(Sweeney et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sti^Z3-5829

(Sweeney et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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