FlyBase Allele Report: Dmel\ct[RNAi.cGa.UAS]

General Information

Symbol

Dmel\ct^RNAi.cGa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0220357

Feature type

allele

Associated gene

Dmel\ct

Associated Insertion(s)

Carried in Construct

P{UAS-ct.RNAi.G}

Key Links

Transgenic product class

RNAi_reagent

(Casso et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Casso et al., 2008)

Mutagen

in vitro construct

(Casso et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-ct.RNAi.G}

Cytology

Description

UAS regulatory sequences drive expression of a construct which produces RNAi directed against ct.

(Casso et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Wong et al., 2014)

CEA with Delta^UAS.cDa

(Zhai et al., 2012)

model of hematologic cancer

CEA with Delta^UAS.cDa, lola^GS88A8, psq^GS88A8

(Zhai et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Delta^UAS.cDa

(Zhai et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia, with Scer\GAL4^Pxn.PS

(Wong et al., 2014)

melanotic mass phenotype, with Scer\GAL4^Pxn.PS

(Wong et al., 2014)

visible, with Scer\GAL4^arm.PS

(Zhai et al., 2012)

visible, with Scer\GAL4^ey.PU

(Zhai et al., 2012)

visible, with Scer\GAL4^vg.PM

(Casso et al., 2008)

Phenotype Manifest In

embryonic/larval lymph gland, with Scer\GAL4^Pxn.PS

(Wong et al., 2014)

eye, with Scer\GAL4^ey.PU

(Zhai et al., 2012)

interommatidial bristle, with Scer\GAL4^arm.PS

(Zhai et al., 2012)

wing margin, with Scer\GAL4^vg.PM

(Casso et al., 2008)

Detailed Description

Statement

Reference

Down-regulation of ct in differentiating blood cells, through expression of ct^{dsRNA.cGa.Scer\UAS}, under the control of Scer\GAL4^Pxn.PS results in blood cell hyperplasia and overgrowth of the lymph glands.

Just under 80% of pupae expressing ct^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS exhibit visible melanotic tumours.

(Wong et al., 2014)

Expression of ct^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^ey.PU results in an overall reduction in the size of the eye compared to wild type.

Expression of ct^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^arm.PS results in loss of ommatidial bristles.

(Zhai et al., 2012)

Expression of ct^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^vg.PM produces a jagged wing-blade margin.

(Casso et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PU, ct^RNAi.cGa.UAS has neoplasia phenotype, enhanceable by BacA\p35^UAS.cUa, Scer\GAL4^ey.PU

(Zhai et al., 2012)

Suppressed by

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PU, ct^RNAi.cGa.UAS has neoplasia phenotype, suppressible | partially by Pi3K92E^KK102291, Scer\GAL4^ey.PU

(Zhai et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^vg.PM, ct^RNAi.cGa.UAS has visible phenotype, non-suppressible by swm^NIG.10084R/Scer\GAL4^vg.PM

(Casso et al., 2008)

Scer\GAL4^vg.PM, ct^RNAi.cGa.UAS has visible phenotype, non-suppressible by swm^37Dh-1

(Casso et al., 2008)

Enhancer of

Statement

Reference

ct^RNAi.cGa.UAS/BacA\p35^UAS.cUa, Scer\GAL4^ey.PU is an enhancer of neoplasia phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PU, lola^GS88A8, psq^GS88A8

(Zhai et al., 2012)

ct^RNAi.cGa.UAS, Scer\GAL4^ey.PU is an enhancer of neoplasia phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PU, lola^GS88A8, psq^GS88A8

(Zhai et al., 2012)

Other

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PU, ct^RNAi.cGa.UAS has neoplasia phenotype

(Zhai et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PU, ct^RNAi.cGa.UAS has eye phenotype, enhanceable by BacA\p35^UAS.cUa, Scer\GAL4^ey.PU

(Zhai et al., 2012)

Suppressed by

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PU, ct^RNAi.cGa.UAS has eye phenotype, suppressible | partially by Pi3K92E^KK102291, Scer\GAL4^ey.PU

(Zhai et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^vg.PM, ct^RNAi.cGa.UAS has wing margin phenotype, non-suppressible by swm^NIG.10084R/Scer\GAL4^vg.PM

(Casso et al., 2008)

Scer\GAL4^vg.PM, ct^RNAi.cGa.UAS has wing margin phenotype, non-suppressible by swm^37Dh-1

(Casso et al., 2008)

Enhancer of

Statement

Reference

ct^RNAi.cGa.UAS/BacA\p35^UAS.cUa, Scer\GAL4^ey.PU is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PU, lola^GS88A8, psq^GS88A8

(Zhai et al., 2012)

ct^RNAi.cGa.UAS, Scer\GAL4^ey.PU is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PU, lola^GS88A8, psq^GS88A8

(Zhai et al., 2012)

Other

Statement

Reference

Delta^UAS.cDa, Scer\GAL4^ey.PH, ct^RNAi.cGa.UAS has eye phenotype

(Wong et al., 2014)

Delta^UAS.cDa, Scer\GAL4^ey.PU, ct^RNAi.cGa.UAS has eye phenotype

(Zhai et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Dl^Scer\UAS.cDa with ct^{dsRNA.cGa.Scer\UAS}, under the control of Scer\GAL4^ey.PH results in dramatically altered eyes.

(Wong et al., 2014)

Flies co-expressing ct^{dsRNA.cGa.Scer\UAS} and Dl^Scer\UAS.cDa under the control of Scer\GAL4^ey.PU show a low frequency of primary eye tumours and secondary tumours. The incidence of primary and secondary tumours is enhanced by co-expression of BacA\p35^Scer\UAS.cUa.

Co-expression of Pi3K92E^KK102291 partially suppresses the tumorous eye growth seen in flies co-expressing ct^{dsRNA.cGa.Scer\UAS} and Dl^Scer\UAS.cDa under the control of Scer\GAL4^ey.PU.

72.5% of flies expressing Dl^Scer\UAS.cDa, psq^GS88A8 and lola^GS88A8 under the control of Scer\GAL4^ey.PU have eye tumours, with 4.9% having macroscopically visible secondary tumour growths derived from the developing retina. The incidence of primary and secondary tumours in these "eyeful" flies is slightly enhanced by co-expression of ct^{dsRNA.cGa.Scer\UAS}, but is greatly enhanced if the "eyeful" flies also co-express both ct^{dsRNA.cGa.Scer\UAS} and BacA\p35^Scer\UAS.cUa.

(Zhai et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ct^RNAi.cGa.UAS

ct^{dsRNA.cGa.Scer\UAS}

ct^{dsRNA.cGa.UAS}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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