Allele Dmel\Ir84a^Scer\UAS.cBa

General Information
Symbol	Dmel\Ir84a^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Benton	FlyBase ID	FBal0220387
Feature type	allele	Associated gene	Dmel\Ir84a

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Benton et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the full length Ir84a open reading frame. (Benton et al., 2009)
Carried in construct	P{UAS-Ir84a.B} (Benton et al., 2009, Benton et al., 2009, Abuin et al., 2011, Grosjean et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	smell perception defective, with Scer\GAL4^Or35a.PF (Benton et al., 2009)
Phenotype Manifest In
	sensillum coeloconicum of antennal segment 3, with Scer\GAL4^Or35a.PF (Benton et al., 2009)
Detailed Description
	Statement Reference Flies expressing Ir84a[Scer\UAS.cBa] under the control of Scer\GAL4[Or35a.PF] results in a strong olfactory response to phenylacetaldehyde in the ac3 sensilla (a response not seen in the wild-type ac3 sensilla), although a response to phenylacetonitrile is not seen in the ac3 sensilla in these flies. (Benton et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Ir84a^Scer\UAS.cBa, Ir8a^{Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP}, Scer\GAL4^Or22a.7.717 has neurophysiology defective phenotype (Abuin et al., 2011)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Neurons co-expressing Ir84a[Scer\UAS.cBa] and Ir8a[Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or22a.7.717] respond to phenylacetaldehyde in a concentration-dependent manner. Neurons co-expressing Ir84a[Scer\UAS.cBa] and Ir8a[ΔATD.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or22a.7.717] fail to respond to phenylacetaldehyde. Neurons co-expressing Ir8a[ΔC.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] and Ir8a[Scer\UAS.cAa] under the control of Scer\GAL4[Or22a.7.717] fail to respond to phenylacetaldehyde. Neurons co-expressing Ir84a[Scer\UAS.cBa] and Ir8a[R481A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or22a.7.717] respond to phenylacetaldehyde in a concentration-dependent manner. Neurons co-expressing Ir84a[Scer\UAS.cBa] and Ir8a[R481E.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or22a.7.717] show a moderate but significant reduction in phenylacetaldehyde responses. Neurons co-expressing Ir84a[Scer\UAS.cBa] and Ir8a[T645A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or22a.7.717] respond to phenylacetaldehyde in a concentration-dependent manner. Neurons co-expressing Ir84a[Scer\UAS.cBa] and Ir8a[D724A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or22a.7.717] fail to respond to phenylacetaldehyde. (Abuin et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^Ir84a-GAL4/Ir84a^Scer\UAS.cBa rescues Ir84a^GAL4 (Grosjean et al., 2011)
Partially rescues	Scer\GAL4^Ir84a-GAL4/Ir84a^Scer\UAS.cBa partially rescues Ir84a^GAL4 (Grosjean et al., 2011)
Comments	Expression of Ir84a[Scer\UAS.cBa] using Scer\GAL4[Ir84a-GAL4] restores the response to phenylacetic acid and phenylacetaldehyde in Ir84a[GAL4] homozygotes. The reduced courtship seen in Ir84a[GAL4] males is also rescued. The increased courtship seen in wild type males when killed female objects are perfumed with phenylacetic acid (or fly food) is not observed in Ir84a[GAL4] males, but this is partially restored by expression of Ir84a[Scer\UAS.cBa]. (Grosjean et al., 2011)
Stocks ( 1 )
Bloomington	41740 w^*; P{UAS-Ir84a.B}attP40; TM2/TM6B, Tb¹
Notes on Origin
Discoverer

Comments
	Generated using the phiC31-based integration system. (Benton et al., 2009)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Ir84a^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Benton
Secondary FlyBase IDs

References ( 4 )
Research paper	Abuin et al., 2011, Neuron 69(1): 44--60 Functional architecture of olfactory ionotropic glutamate receptors. [FBrf0212725] Grosjean et al., 2011, Nature 478(7368): 236--240 An olfactory receptor for food-derived odours promotes male courtship in Drosophila. [FBrf0216424] Benton et al., 2009, Cell 136(1): 149--162 Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila. [FBrf0206496]
Supplementary material	Benton et al., 2009, Cell 136(1): Supplemental Experimental Procedures. [FBrf0207607]

Allele Dmel\Ir84aScer\UAS.cBa

Allele Dmel\Ir84a^Scer\UAS.cBa