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General Information
Symbol
Dmel\Atg13
Species
D. melanogaster
Name
FlyBase ID
FBal0220537
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
unc-513
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Imprecise excision of the P{EP}Atg1EP3348 insertion resulting in a deletion that extends 804 bp downstream of the insertion site.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{EP}Atg1EP3348has deleted 804bp downstream from the insertion point.

Imprecise excision of the insertion in Atg1EP3348, resulting in a deletion of 804bp downstream from the insertion point.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Atg13 homozygous third instar larval mushroom bodies show prevalent disintegration of the core into multiple bundles and defects in the extension of calix dendrites, as compared to controls.

Atg13 homozygous clones within mosaic third instar larval mushroom bodies show prevalent peduncle defects (split fascicles are observed in nearly half of cases) and defects in the extension of calix dendrites, although both the dorsal and the medial lobes do not show defects, as compared to control clones. The majority of Atg13 homozygous lateral neuroblast clones lead to antennal lobe projection neurons fasciculation defects, although most of the anterodorsal neuroblast clones do not lead to obvious defects in axonal projections, as compared to control clones. Projection neurons clones show mild to severe ectopic innervation of the off-target glomeruli DA1, DM5 and DM2 glomeruli, but do not affect the VA2, VA1d, VA1lm, VA3, VM2, DM6, DL1 or VM7 glomeruli, as compared to controls.

Atg13 homozygous DL1 clones lead to a small but significant increase in the number of branches in the calyx, but not in the number of terminal branches in the lateral horn, as compared to clones; a significant proportion of Atg13 homozygous adult antennal lobe projection neuron clones (anterodorsal neuroblast clones, lateral neuroblast clones or ventral neuroblast clones) exhibit overextending dendrites, overshooting as compared to controls.

Atg13 heterozygous neuromuscular junctions exhibit wild-type bouton levels.

Marker analysis indicates that pre-and post synaptic component of the neuromuscular junction fail to align properly in Atg13 homozygous or Atg125/Atg13 heterozygous larvae. In addition, in Atg13 homozygotes the size of neuromuscular junctions (NMJs) is reduced, as is the density of puncta within these junctions. This reduction in size appears not to be due to retraction of an initially normal sized NMJ, as there is not sign of junctional proteins on the muscle surface outside of the NMJ. Ultrastructural analysis reveals that, while many aspects ultrastructure of boutons in these mutants, they have lower densities of active zones, t-bars and active zones containing t-bars. These structural defects in NMJs are accompanied by an approximately 3 fold decrease in the amplitude of evoked excitatory junction potentials (EJPs). There is no accompanying change in spontaneous mini excitatory junction potential (mEJP) amplitude. Atg13/Atg1EY07351 NMJs show the same electrophysiological defects and also exhibit a significant decrease in mEJP frequency.

Accumulation of synaptic material is seen in the axons of motor neurons in Atg13 homozygous larvae.

Atg13/Atg125 and Atg13/Df(3L)ED4486 animals show axonal transport defects at the larval stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Atg13 has axon & motor neuron | larval stage phenotype, non-suppressible by rl1/rl1

Suppressor of
Statement
Reference

Atg13/Atg1[+] is a suppressor of synapse phenotype of Rae1EX28

Atg13/Atg1[+] is a suppressor of NMJ bouton phenotype of Rae1EX28

NOT Suppressor of
Statement
Reference

Atg125/Atg13 is a non-suppressor of neuroblast | larval stage phenotype of fzy1/fzy5032

Additional Comments
Genetic Interactions
Statement
Reference

Atg125/Atg13 does not suppress the neuroblast loss seen in fzy5032/fzy1 mutant larval brains.

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is partially suppressed if the flies are also carrying Atg13.

An Atg13 heterozygous background dominantly suppresses the increase in bouton number seen in Rae1EX28 homozygous mutant neuromuscular junction.

The structural defects seen in the neuromuscular junctions (NMJ) of Atg13 homozygous larvae are almost completely suppressed by rl1/rl1 : synaptic density and apposition of pre and post synaptic components are restored and NMJ size is partially restored. Despite the fact that evoked excitatory junction potentials at larval NMJs are decreased in both rl1 homozygous and Atg13 homozygous larvae, this phenotype is not see in rl1 Atg13 double homozygotes.

The accumulation of synaptic material in the axons of of motor neurons in Atg13 homozygous larvae is not suppressed by rl1/rl1.

Unc-76ex107/+ ; Atg13/+ double heterozygous larvae have more synaptic vesicle aggregates in the segmental nerve than either single heterozygote.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)