FlyBase Allele Report: Dmel\lola[JF02254]

General Information

Symbol

Dmel\lola^JF02254

Species

D. melanogaster

Name

FlyBase ID

FBal0220646

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02254}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

(Perkins et al., 2009.3.31)

Progenitor genotype

Carried in construct

P{TRiP.JF02254}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.3.31)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02254

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal anterior ventral multidendritic neuron vdaa, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

abdominal dorsal multidendritic neuron ddaD | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4²²¹

(Hattori et al., 2013)

dendritic tree | third instar larval stage, with Scer\GAL4^ppk.PG

(Hattori et al., 2013)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

larval dorsal multidendritic neuron ddaD, with Scer\GAL4²²¹

(Iyer et al., 2013)

larval dorsal multidendritic neuron ddaE, with Scer\GAL4²²¹

(Iyer et al., 2013)

larval ventral multidendritic neuron vdaB, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

Detailed Description

Statement

Reference

Expression of lola^JF02254 driven by Scer\GAL4^GMR.PU does not significantly affect eye morphology.

(Peng et al., 2016)

Expression of lola^JF02254 in muscle under the control of Scer\GAL4^Mef2.PU results in significant hyperglycaemia.

Expression of lola^JF02254 in the fat body under the control of Scer\GAL4^DcG results in significant hyperglycaemia.

Expression of lola^JF02254 in the fat body under the control of Scer\GAL4^DcG results in significant higher trehalose levels than controls.

(Ugrankar et al., 2015)

Only 50% of males expressing lola^JF02254 under the control of Scer\GAL4^fru.P1.D initiate courtship behaviour towards wild-type females during a 1 hour observation period, and those that do court show a significant delay.

(Neville et al., 2014)

The expression of lola^JF02254 under the control of Scer\GAL4²²¹, in combination with Dicer-2 for efficient RNAi, results in third instar larval class I abdominal dorsal multidendritic neurons ddaD and ddaE exhibiting a significant decrease in the number of dendrite branch terminals, as compared to controls.

Expressing lola^JF02254 under the control of Scer\GAL4^ppk.PG, in combination with Dicer-2 for efficient RNAi, results in third intar larval class IV abdominal dorsal multidendritic neurons ddaC exhibiting dendrite branch terminals of similar number but of significantly lower average length, as compared to controls.

(Hattori et al., 2013)

Expression of lola^JF02254 in C-I neurons under the control of Scer\GAL4²²¹ results in ddaD neurons that have an increased number of dendritic branches compared to controls. Dendritic phenotypes are also seen in ddaE neurons. No defects are seen in vpda neurons.

Expression of lola^JF02254 in C-IV neurons under the control of Scer\GAL4^ppk.1.9 results in a reduction in total dendritic length in ddaC, v'ada and vdaB neurons. The total number of dendritic branches is also reduced in ddaC and vdaB neurons.

(Iyer et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

lola^JF02254/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, for^GD6843

(Peng et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

lola^JF02254/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, for^GD6843

(Peng et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of lola^JF02254 suppresses the rough eye phenotype in flies with for^GD6843 driven by Scer\GAL4^GMR.PU. Expression of lola^JF02254 driven by Scer\GAL4^elav.PU partially suppresses targeting defects seen in motor axons of intersegmental nerve b (ISNb) in Df(2L)ED243/Df(2L)ED243 mutant embryos.

(Peng et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26714

y¹ v¹; P{TRiP.JF02254}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lola^JF02254

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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