FlyBase Allele Report: Dmel\Syx18[JF02263]

General Information

Symbol

Dmel\Syx18^JF02263

Species

D. melanogaster

Name

FlyBase ID

FBal0220653

Feature type

allele

Associated gene

Dmel\Syx18

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02263}

Also Known As

JF02263

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

(Perkins et al., 2009.3.31)

Progenitor genotype

Carried in construct

P{TRiP.JF02263}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.3.31)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02263

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

abnormal size | larval stage, with Scer\GAL4^pros-V1

(Lakatos et al., 2019)

decreased occurrence of cell division | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

Phenotype Manifest In

autolysosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Lakatos et al., 2019)

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Lakatos et al., 2019)

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Lakatos et al., 2019)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

embryonic/larval garland cell | larval stage, with Scer\GAL4^pros-V1

(Lakatos et al., 2019)

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Lakatos et al., 2019)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

intermediate neuronal progenitor | decreased number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

larval brain | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

late endosome | larval stage, with Scer\GAL4^pros-V1

(Lakatos et al., 2019)

secretory granule | larval stage, with Scer\GAL4^fkh.PH

(Lakatos et al., 2019)

type II neuroblast | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

Detailed Description

Statement

Reference

Expressing Syx18^JF02263 under the control of Scer\GAL4^pros-V1 results in larger late endosomes in larval garland cells (Rab7-positive puncta in fluorescence microscopy).

Clonal expression of Syx18^JF02263 under the control of Scer\GAL4^Act5C.PP (in combination with Dicer-2, for a more efficient RNAi) affects the distribution of autophagic vesicles in the larval fat body (Atg8-positive puncta in fluorescence microscopy).

Expressing Syx18^JF02263 under the combined control of Scer\GAL4^fkh.PH abolishes glue granules in the larval salivary glands; in agreement, all of the (pre)pupae fell down from the wall of the culture vials.

(Lakatos et al., 2019)

The expression of Syx18^JF02263 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP.

(Ke et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, Syx18^JF02263 has abnormal cell number | larval stage phenotype, suppressible by N^UAS.cMa, Scer\GAL4^wor.PA, Scer\GAL80^ase.PN