FlyBase Allele Report: Dmel\pum[JF02267]

General Information

Symbol

Dmel\pum^JF02267

Species

D. melanogaster

Name

FlyBase ID

FBal0220657

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02267}

Also Known As

pum RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

(Perkins et al., 2009.3.31)

Progenitor genotype

Carried in construct

P{TRiP.JF02267}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.3.31)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02267

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa

(Collins et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of pum^JF02267 under the control of Scer\GAL4^Mhc.PU does not cause any wing posture or jumping ability defects in adult flies and neither does it, when driven by Scer\GAL4^ple.PF, lead to any abnormal mitochondrial aggregations in adult brain dopaminergic (DA) neurons or the number of PPL1 cluster DA neurons.

(Gehrke et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pum^JF02267, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PU/pum^JF02267 is a suppressor | partially of visible | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU/pum^JF02267 is a suppressor | partially of abnormal jumping phenotype of Pink1^B9

(Gehrke et al., 2015)

pum^JF02267, Scer\GAL4^Mhc.PU is a suppressor of visible | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

pum^JF02267, Scer\GAL4^Mhc.PU is a suppressor of abnormal jumping phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/pum^JF02267 is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/pum^JF02267 is a suppressor | partially of decreased cell number | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

pum^JF02267, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

Suppressor of

Statement

Reference

pum^JF02267, Scer\GAL4^Mhc.PU is a suppressor of wing | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

pum^JF02267, Scer\GAL4^Mhc.PU is a suppressor of flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

pum^JF02267, Scer\GAL4^Mhc.PU is a suppressor of mitochondrion | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/pum^JF02267 is a suppressor | partially of dopaminergic neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/pum^JF02267 is a suppressor | partially of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/pum^JF02267 is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU/pum^JF02267 is a suppressor | partially of wing | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU/pum^JF02267 is a suppressor | partially of flight muscle cell phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU/pum^JF02267 is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormal mitochondrial aggregations (observed in flight muscles or dopaminergic neurons), wing posture and jumping ability defects as well as the loss of dopaminergic neurons from the PPL1 cluster characteristic for Pink1^B9 adult homozygotes are significantly improved by expression (controlled by either the Scer\GAL4^Mhc.PU muscle driver or the Scer\GAL4^ple.PF neuronal driver) of pum^JF02267. Similarly, the mitochondrial aggregations in flight muscles along with the wing posture and jumping ability defects observed in adult flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PU are partially rescued by co-expression of pum^JF02267.

(Gehrke et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26725

y¹ v¹; P{TRiP.JF02267}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pum^JF02267

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close