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General Information
Symbol
Dmel\Atg1JF02273
Species
D. melanogaster
Name
FlyBase ID
FBal0220663
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Atg1i, Atg1-IR
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of an inverted repeat.
Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Flies expressing Atg1JF02273 under the control of Scer\GAL4Mef2.PU have similar lifespan, locomotion and mitochondrial structure as wild-type controls.
Expression of Atg1JF02273 under the control of Scer\GAL4phm.PO leads to massive accumulation of lipid droplets in the prothoracic gland cells of third instar larvae.
Expression of Atg1JF02273 in muscle under the control of Scer\GAL4Mef2.PU results in significant hyperglycaemia.
Expression of Atg1JF02273 under the control of Scer\GAL4Myo31DF-NP0001 results in significantly delayed midgut degradation after pupariation, with increased size of the midgut and gastric caeca 4 hrs after puparium formation, and decreased autophagy 2 hrs after puparium formation, as compared to controls. Expression of Atg1JF02273 under the control of Scer\GAL4cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.
Expression of Atg1JF02273 under the control of Scer\GAL4Mef2.PR is able to completely suppress the formation of autophagosomes in the muscles of both untreated and chloroquine-treated larvae starved on low-nutrient food for 6 hours. Glycogen breakdown occurs normally in the muscles of larvae expressing Atg1JF02273 under the control of Scer\GAL4Mef2.PR starved on low-nutrient food for 24 hours, but the rate of breakdown is reduced compared to controls. After 3 or 6 hours of starvation, expression of Atg1JF02273 under the control of Scer\GAL4Mef2.PR significantly improves the crawling defects seen in chloroquine-treated larvae compared to controls. After 6 hours starvation, crawling is negatively affected in the absence of chloroquine treatment. In well-fed larvae there is little difference between crawling time compared to controls, either in the presence or absence of chloroquine.
Expression of Atg1JF02273 using Scer\GAL4Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones. Expression of Atg1JF02273 using Scer\GAL4Cg.PA causes a decrease in starvation-induced autophagic response in the fat body of wandering third instar larvae compared with wild-type.
Expression of Atg1JF02273 via Scer\GAL4HCH.Hand causes a decline in cardiac performance. Expression of Atg1JF02273 via Scer\GAL4Mef2.PR causes mitochondrial abnormalities and degeneration in skeletal muscle.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Expression of Atg1JF02273 completely suppresses the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.
Co-expression of Atg1JF02273 and TorHMS00904 under the control of Scer\GAL4Myo31DF-NP0001 restores autophagy and gastric caeca contraction to levels similar to those seen in controls.
The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv is not suppressed if they are also co-expressing Atg1JF02273.
In contrast to the expression of either transgene alone, glycogen breakdown is disrupted in the muscles of larvae co-expressing Atg1JF02273 and GlyPNIG.7254R under the control of Scer\GAL4Mef2.PR and starved on low-nutrient food for 24 hours.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Atg1JF02273
Atg1[JF02273]
Name Synonyms
Secondary FlyBase IDs
    References (17)