FB2025_01 , released February 20, 2025
Allele: Dmel\Atg1JF02273
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General Information
Symbol
Dmel\Atg1JF02273
Species
D. melanogaster
Name
FlyBase ID
FBal0220663
Feature type
allele
Associated gene
Dmel\Atg1
Associated Insertion(s)
Carried in Construct
P{TRiP.JF02273}
Also Known As
Atg1 RNAi, UAS-atg1 RNAi, UAS-Atg1-RNAi, Atg1-RNAi, atg1RNAi, UAS-atg1RNAi, UAS-Atg1i, Atg1-IR
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Perkins et al., 2009.3.31)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Perkins et al., 2009.3.31)
Mutagen
in vitro construct
(Perkins et al., 2009.3.31)
Progenitor genotype
Carried in construct
P{TRiP.JF02273}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.3.31)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-JF02273
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell number | P-stage, with Scer\GAL4Mef2.247

      (Zhang et al., 2024)

      abnormal cell number | P-stage, with Scer\GAL4phtm.PO, Scer\GAL80spok.1.45

      (Zhang et al., 2024)

      abnormal locomotor behavior | adult stage |  :chemical conditional | nutrition conditional

      (Zirin et al., 2013)

      abnormal locomotor behavior | third instar larval stage, with Scer\GAL4elav.PU

      (Zhu et al., 2021)

      abnormal size | P-stage, with Scer\GAL4NP0001

      (Xu et al., 2015)

      abnormal starvation stress response, with Scer\GAL4Mef2.PR

      (Zirin et al., 2013)

      abnormal starvation stress response | adult stage, with Scer\GAL4Cg.PU

      (Bourouis et al., 2019)

      abnormal starvation stress response | larval stage, with Scer\GAL4Cg.PU

      (Xu et al., 2015)

      abnormal starvation stress response | late third instar larval stage, with Scer\GAL4Cg.PA

      (Pircs et al., 2012)

      decreased cell death | P-stage, with Scer\GAL4NP0001

      (Xu et al., 2015)

      increased cell size | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4Act5C.PU

      (Bourouis et al., 2019)

      increased occurrence of cell division | adult stage, with Scer\GAL4NP0001

      (Nassari et al., 2022)

      increased size | adult stage, with Scer\GAL4Bx-MS1096

      (Tyra et al., 2020)

      increased size | adult stage, with Scer\GAL4en-e16E

      (Tyra et al., 2020)

      increased size | adult stage, with Scer\GAL4nub-AC-62

      (Tyra et al., 2020)

      short lived | calorie restriction conditional, with Scer\GAL4Cg.PU

      (Bourouis et al., 2019)

      visible | adult stage, with Scer\GAL4Bx-MS1096

      (Tyra et al., 2020)

      visible | adult stage, with Scer\GAL4en-e16E

      (Tyra et al., 2020)

      visible | adult stage, with Scer\GAL4nub-AC-62

      (Tyra et al., 2020)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Clonal expression of Atg1JF02273 under the control of Scer\GAL4Act5C.PU in the larval fat body leads to smaller cells in starved individuals, but not in well fed individuals, as compared to controls.

      (Bourouis et al., 2019)

      Flies expressing Atg1JF02273 under the control of Scer\GAL4Mef2.PU have similar lifespan, locomotion and mitochondrial structure as wild-type controls.

      (Xu et al., 2019)

      Expression of Atg1JF02273 under the control of Scer\GAL4phm.PO leads to massive accumulation of lipid droplets in the prothoracic gland cells of third instar larvae.

      (Danielsen et al., 2016)

      Expression of Atg1JF02273 in muscle under the control of Scer\GAL4Mef2.PU results in significant hyperglycaemia.

      (Ugrankar et al., 2015)

      Expression of Atg1JF02273 under the control of Scer\GAL4Myo31DF-NP0001 results in significantly delayed midgut degradation after pupariation, with increased size of the midgut and gastric caeca 4 hrs after puparium formation, and decreased autophagy 2 hrs after puparium formation, as compared to controls.

      Expression of Atg1JF02273 under the control of Scer\GAL4cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

      (Xu et al., 2015)

      Expression of Atg1JF02273 under the control of Scer\GAL4Mef2.PR is able to completely suppress the formation of autophagosomes in the muscles of both untreated and chloroquine-treated larvae starved on low-nutrient food for 6 hours.

      Glycogen breakdown occurs normally in the muscles of larvae expressing Atg1JF02273 under the control of Scer\GAL4Mef2.PR starved on low-nutrient food for 24 hours, but the rate of breakdown is reduced compared to controls.

      After 3 or 6 hours of starvation, expression of Atg1JF02273 under the control of Scer\GAL4Mef2.PR significantly improves the crawling defects seen in chloroquine-treated larvae compared to controls. After 6 hours starvation, crawling is negatively affected in the absence of chloroquine treatment. In well-fed larvae there is little difference between crawling time compared to controls, either in the presence or absence of chloroquine.

      (Zirin et al., 2013)

      Expression of Atg1JF02273 using Scer\GAL4Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

      Expression of Atg1JF02273 using Scer\GAL4Cg.PA causes a decrease in starvation-induced autophagic response in the fat body of wandering third instar larvae compared with wild-type.

      (Pircs et al., 2012)

      Expression of Atg1JF02273 via Scer\GAL4HCH.Hand causes a decline in cardiac performance.

      Expression of Atg1JF02273 via Scer\GAL4Mef2.PR causes mitochondrial abnormalities and degeneration in skeletal muscle.

      (Lee et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Atg1JF02273 completely suppresses the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

      (Kim et al., 2015)

      Co-expression of Atg1JF02273 and TorHMS00904 under the control of Scer\GAL4Myo31DF-NP0001 restores autophagy and gastric caeca contraction to levels similar to those seen in controls.

      (Xu et al., 2015)

      The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv is not suppressed if they are also co-expressing Atg1JF02273.

      (Ibar et al., 2013)

      In contrast to the expression of either transgene alone, glycogen breakdown is disrupted in the muscles of larvae co-expressing Atg1JF02273 and GlyPNIG.7254R under the control of Scer\GAL4Mef2.PR and starved on low-nutrient food for 24 hours.

      (Zirin et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Atg1JF02273
      (Billes et al., 2018, Clark et al., 2018, Wang et al., 2012)
      Atg1[JF02273]
      (Nagy et al., 2015)
      atg1RNAi
      (Zhuang et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (50)