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General Information
Symbol
Dmel\Gem3e03688
Species
D. melanogaster
Name
FlyBase ID
FBal0220796
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Insertion 92bp upstream of the transcription start site. Part of the 5' and 3' ends of the original PBac{RB} element have been lost during the insertion.

    Insertion components
    PBac{?RB}Gem3e03688
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    30% of homozygous larvae survive to day 8 after egg laying (a period when the control larvae have already pupated) but fail to pupate, although a few animals form pseudopupal cases. A small fraction of homozygous larvae survive to day 25 after egg laying.

    Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying.

    Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.

    Gem3rL562/Gem3e03688 animals survive to the prepupal stage. The animals undergo a prolonged wandering third instar larval stage.

    Gem3rL562/Gem3e03688 animals show normal body wall contraction rates throughout the first and second larval instars, but at the third instar larval stage the body wall contraction rate is significantly decreased in the mutant animals compared to controls. The mutant third instar larvae show no apparent disruption in the gross structure of the body wall musculature or in sarcomere organisation.

    The puparium made by Gem3rL562/Gem3e03688 animals fails to evert spiracles and has a large axial ratio (length/width).

    Gem3rL562/Gem3e03688 larvae show a synaptic overgrowth phenotype at the neuromuscular junction (NMJ) and have a significantly increased synaptic area when it is normalised to muscle size.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT suppressed by
    Statement
    Reference
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer

    The lethality of the homozygous Gem3e03688 chromosome cannot be rescued by expression of Gem3Scer\UAS.T:Avic\GFP-CFP under the control of Scer\GAL41032.hx, suggesting that there may be a secondary lethal mutation on the Gem3e03688 chromosome (since the Gem3Scer\UAS.T:Avic\GFP-CFP, Scer\GAL41032.hx combination can rescue the lethality of Gem3e03688/Gem3rL562 and Gem3rL562/Gem3rL562 animals).

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    Name Synonyms
    Secondary FlyBase IDs
      References (2)