30% of homozygous larvae survive to day 8 after egg laying (a period when the control larvae have already pupated) but fail to pupate, although a few animals form pseudopupal cases. A small fraction of homozygous larvae survive to day 25 after egg laying.
Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying.
Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.
Gem3rL562/Gem3e03688 animals survive to the prepupal stage. The animals undergo a prolonged wandering third instar larval stage.
Gem3rL562/Gem3e03688 animals show normal body wall contraction rates throughout the first and second larval instars, but at the third instar larval stage the body wall contraction rate is significantly decreased in the mutant animals compared to controls. The mutant third instar larvae show no apparent disruption in the gross structure of the body wall musculature or in sarcomere organisation.
The puparium made by Gem3rL562/Gem3e03688 animals fails to evert spiracles and has a large axial ratio (length/width).
Gem3rL562/Gem3e03688 larvae show a synaptic overgrowth phenotype at the neuromuscular junction (NMJ) and have a significantly increased synaptic area when it is normalised to muscle size.