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General Information
Symbol
Dmel\Gem3ΔN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0220955
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gem3BART, UAS.Gem3BART
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the C-terminus of Gem3 (the N-terminal 424 amino acid residues are missing).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of one copy of Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR has no effect on both motor function and survival throughout adulthood; expression of two copies of Gem3ΔN.Scer\UAS results in the majority of flies being flightless (5 days old) and flight ability progressively declines (none fly by day 15) and there is a progressive drop in adult survival.

Flies expressing Gem3ΔN.Scer\UAS (co-expressed with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4Mef2.PR show an age-dependent reduction in climbing ability compared to controls. The majority of flies are flightless at 5 days post-eclosion.

Flies co-expressing Gem3Scer\UAS.cCa, Gem3GD17221 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR are flight defective.

Animals expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4how-24B show several defects at metamorphosis; the flies are "headless" as a results of failed head eversion and the wings are shortened. The pupae are elongated compared to wild type. The abdomen remains elongated (as in the larval stage) during pupal development in these animals, although its shows normal segmentation as shown by the complete bristle pattern on the tergites. The leg discs evert but the legs are not fully elongated.

Eclosed flies expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR have a reduced ability to fly, although they can walk and jump normally. The indirect flight muscles in these animals are shrunken, resulting in increased spacing, and breakages and large tears can be seen between the muscle fibres.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Adult flies bearing Df(1)ED6716 and also expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR display rapid age-progressive decrease in viability as well as flight ability relative to controls.

Expression of Gem3ΔN.Scer\UAS together with GlosGD17786 under the Scer\GAL4Mef2.PR driver are adult lethal and simultaneous expression of Gem3ΔN.Scer\UAS and any of the following: GlosGD17786, rigNIG.11171R, rigScer\UAS.T:Avic\GFP-ECFP, Gem2Scer\UAS.cBa, SmndsRNA.C.Scer\UAS.WIZ or SmnScer\UAS.T:Zzzz\FLAG leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium.

Scer\GAL4elav-C155-driven co-expression of Gem3ΔN.Scer\UAS and GlosGD17786, Gem2Scer\UAS.cBa, SmndsRNA.C.Scer\UAS.WIZ or SmnScer\UAS.T:Zzzz\FLAG reduced adult viability and Gem3ΔN.Scer\UAS;rigScer\UAS.T:Avic\GFP-ECFP flies are completely lethal.

Flies expressing simultaneously VletGD5216 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR display significantly reduced survival rate compared to controls.

Df(3R)Exel6178 adult heterozygotes expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR display age-progressive loss of flight ability.

No decline in adult flight ability compared to controls is observed when wmdKK100974 and Gem3ΔN.Scer\UAS are co-expressed under the Scer\GAL4Mef2.PR driver.

Expression of Tgs1GD14932 together with Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR leads to age-progressive flight defects.

Flies expressing both iclnScer\UAS.cBa and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR are completely lethal.

Co-expression of Gem3ΔN.Scer\UAS and rigScer\UAS.T:Avic\GFP-ECFP or rigΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in flightless flies with shortened lifespans (compared to either single expression, which is similar to controls).

Co-expression of rigNIG.11171R or SmnScer\UAS.T:Avic\GFP or SmndsRNA.N.Scer\UAS.WIZ or SmndsRNA.FL.Scer\UAS.WIZ or Gem2ΔN.Scer\UAS or Gem2Scer\UAS.cBa and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in lethality.

Expression of one copy of Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR in a Df(2R)exu1/+ background results in progressive flight defects and reduced adult viability. Presence of SmnX7 or Df(3L)ED4782 does not result in significant progressive flight or survival phenotypes in flies with expression of Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR.

Co-expression of SmnΔ6.Scer\UAS.T:Avic\GFP and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in flightless flies and a significant drop in adult viability throughout adulthood. Co-expression of SmnScer\UAS.T:Zzzz\FLAG and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in partial lethality, with the few escapers exhibiting severe flight defects. Co-expression of Gem3ΔN.Scer\UAS and SmndsRNA.C.Scer\UAS.WIZ driven by Scer\GAL4Mef2.PR results in flight defects and reduces adult viability, compared to controls.

Co-expression of Gem3ΔN.Scer\UAS and Gem2ΔC.Scer\UAS driven by Scer\GAL4Mef2.PR results in progressive age-dependent flight defects (no significant effect on survival with age progression). Co-expression of Gem3ΔN.Scer\UAS and one copy of Gem2NIG.10419R driven by Scer\GAL4Mef2.PR results in flight defects and partial lethality; co-expression of Gem3ΔN.Scer\UAS and two copies of Gem2NIG.10419R driven by Scer\GAL4Mef2.PR results in lethality.

Expression of SmnScer\UAS.T:Avic\GFP-YFP.Venus is unable to suppress the flight defects seen when Gem3ΔN.Scer\UAS is expressed under the control of Scer\GAL4Mef2.PR.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Gem3Scer\UAS.cCa rescues the flight defects seen in flies expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR.

Expression of Gem3Scer\UAS.cCa rescues the lethality seen in flies co-expressing Gem3GD17221 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR. The rescued flies exhibit flight defects.

Pupae expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4how-24B have increased axial ratios.

The lethality caused by expression of Gem3ΔN.Scer\UAS under the control of one of Scer\GAL4Act5C.PU, Scer\GAL4c179, Scer\GAL4how-24B, Scer\GAL4G7, Scer\GAL4Mef2.PR or Scer\GAL4C57 is significantly suppressed by co-expression of Gem3Scer\UAS.cCa.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Gem3ΔN.Scer\UAS
Gem3ΔN.UAS
Name Synonyms
Secondary FlyBase IDs
    References (6)