FlyBase Allele Report: Dmel\polybromo[Δ86]

General Information

Symbol

Dmel\polybromo^Δ86

Species

D. melanogaster

Name

FlyBase ID

FBal0221008

Feature type

allele

Associated gene

Dmel\polybromo

Associated Insertion(s)

Carried in Construct

Also Known As

Bap180^Δ86

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Carrera et al., 2008)

Progenitor genotype

polybromo^EY14080

(Carrera et al., 2008)

Cytology

Description

Imprecise excision of the P{EPgy2}polybromo^EY14080 insertion, resulting in a 575bp deletion that removes the 5'UTR and extends 120bp into the coding sequence.

(Carrera et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:24,805,053..24,805,627 [-]

Comment:

A 575bp deletion resulting from the imprecise excision of the P{EPgy2}polybromo^EY14080 insertion.

(Carrera et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation | dominant, with In(1)w^m4

(Nakayama et al., 2012)

female sterile

(Carrera et al., 2008)

homeotic

(Nakayama et al., 2012)

lethal - all die during embryonic stage | maternal effect

(Carrera et al., 2008)

some die during embryonic stage | maternal effect

(Carrera et al., 2008)

viable

(Carrera et al., 2008)

Phenotype Manifest In

adult abdominal segment 5 | ectopic

(Nakayama et al., 2012)

adult abdominal segment 6

(Nakayama et al., 2012)

egg chorion

(Carrera et al., 2008)

vitelline membrane

(Carrera et al., 2008)

Detailed Description

Statement

Reference

polybromo^Δ86/+ enhances the position effect variegation at the w locus which is seen in In(1)w^m4 flies.

4% of heterozygous adult males and 40% of homozygous adult males show transformation of A6 to A5.

(Nakayama et al., 2012)

Homozygotes can survive to the adult stage with no obvious morphological defects.

Homozygous females are sterile; the eggs they lay fail to hatch and have eggshell defects. Eggs laid by homozygous females have very thin and irregularly deposited chorions and dechorionated embryos are permeable to the dye neutral red, indicating defects in the barrier function of the vitelline membrane.

24.3% of eggs produced by females containing homozygous germ line clones hatch. Embryos derived from female germ line clones and lacking both maternal and zygotic polybromo function die at a range of developmental stages, but some are able to develop into normal adults.

Incorporation of BrdU at sites of chorion gene amplification appears normal in follicle cell nuclei.

(Carrera et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN, polybromo^Δ86 has visible | dominant phenotype

(Curtis et al., 2011)

Bap170^Δ65, polybromo^Δ86 has lethal | pupal stage phenotype

(Carrera et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

akirin^KG01343, polybromo[+]/polybromo^Δ86 has larval somatic muscle cell phenotype

(Nowak et al., 2012)

Su(var)3-3[+]/Su(var)3-3^ΔN, polybromo^Δ86 has wing vein | ectopic phenotype

(Curtis et al., 2011)

Bap170^Δ65, polybromo^Δ86 has ventral denticle belt phenotype

(Carrera et al., 2008)

Bap170^Δ65, polybromo^Δ86 has embryonic/larval crystal cell phenotype

(Carrera et al., 2008)

Bap170^Δ65, polybromo^Δ86 has leg | pupal stage phenotype

(Carrera et al., 2008)

Bap170^Δ65, polybromo^Δ86 has melanotic mass | pupal stage phenotype

(Carrera et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Double heterozygosity for both polybromo^Δ86 and Trl^R85 enhances the position effect variegation at the w locus which is seen in In(1)w^m4 flies more strongly than either single polybromo^Δ86 and Trl^R85 heterozygote alone.

12% of Trl^R85/polybromo^Δ86 double heterozygous adult males show transformation of A6 to A5.

15% of polybromo^Δ86/Df(3L)Aprt-21 double heterozygous adult males show transformation of A6 to A5.

(Nakayama et al., 2012)

Stage 16 polybromo^Δ86/+ ; akirin^KG01343/+ double heterozygous embryos show disruption of the somatic muscle pattern (38% of embryos have missing, misattached or duplicated muscles in at least 2 hemisegments).

(Nowak et al., 2012)

Bap170^Δ65 polybromo^Δ86 double mutants show fully penetrant pupal lethality, which can be rescued by ubiquitous expression of either polybromo^Scer\UAS.cCa or Bap170^Scer\UAS.cCa.

Bap170^Δ65 polybromo^Δ86 double mutant larvae show overproduction of crystal cells.

Bap170^Δ65 polybromo^Δ86 double mutants show defects at the pupal stage beginning approximately 12 hours after puparium formation; the mutants fail to fully evert their legs and sometimes have melanotic patches.

Embryos laid by Bap170^Δ65 females containing polybromo^Δ86 homozygous germ line clones die before the third larval instar. Cuticles secreted by the embryos that fail to hatch show dorsalisation (ventral denticle belts are reduced), especially in the anterior region.

Bap170^Δ65 females with osa³⁰⁸ polybromo^Δ86 germ lines clones are able to differentiate oocytes.

(Carrera et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

polybromo^Δ86 is rescued by polybromo^{Hsp83.Tag:FLAG}

(Nakayama et al., 2012)

Partially rescued by

polybromo^Δ86 is partially rescued by Scer\GAL4^T155/polybromo^UAS.cCa

(Carrera et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63127

Bap170^Δ65; P{neoFRT}82B polybromo^Δ86/T(2;3)SM6a-TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Bap180^Δ86

(Curtis et al., 2011, Di Stefano et al., 2011)

bap180^Δ86

(He et al., 2017, Hong and Choi, 2016, Nowak et al., 2012, Kirilly et al., 2011, Carrera et al., 2008)

bap180Δ86

(Nakayama et al., 2012)

polybromo^Δ86

(Tian and Smith-Bolton, 2021)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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