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General Information
Symbol
Dmel\ApplAβ.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0221089
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dAβ
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Appl amino acid residues 767-831, which are tagged with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ApplAβ.Scer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4c739 or Scer\GAL4VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair middle-term memory (measured 2hr post-training) but strongly reduces long-term memory (measured 24hr after training) tested in an aversive olfactory memory assay.

Adult specific expression of ApplAβ.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4Mef2.247.Switch (activated in adult flies fed RU486) does not significantly affect short term memory in an olfactory aversive conditioning paradigm, compared to controls.

Adult specific expression of ApplAβ.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4Mef2.247.Switch (activated in adult flies fed RU486) significantly enhances short term memory defects in Appld/+ flies (compared to Appld/+ flies or to controls not fed RU486); there are no significant effects on learning, olfactory acuity or shock reactivity.

30 day old flies expressing ApplAβ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF have aggregates in the retina (these aggregates are not seen in wild-type controls). These aggregates have characteristics of amyloid deposits. These 30 day old flies also show extensive vacuolisation of the retina.

Expression of ApplAβ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a results in the presence of toluidine blue-positive deposits, vacuoles and dark shrunken nuclei in the brains of 30 day old flies. No gross abnormalities are seen in these flies at 2 days after eclosion.

6 day old flies expressing ApplAβ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155 have a significantly lower performance index in a fast phototaxis assay than control flies and the performance continues to decline with age in the mutant flies.

10 day old flies expressing ApplAβ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a have fibrillar structures within the axons.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The impaired long-term memory tested in an aversive olfactory memory assay (measured 24hr post-training) characteristic for adult flies expressing ApplAβ.Scer\UAS.T:Ivir\HA1 (combined with tub-Gal80[ts] to limit expression to adulthood) under the control of either Scer\GAL4c739 or Scer\GAL4VT064246 is not significantly affected by co-expression of Nep1EY21255 with the Scer\GAL4c739 driver but is effectively rescued with the Scer\GAL4VT064246 driver, whereas co-expression of Nep1mut.Scer\UAS (with Scer\GAL4VT064246) has no effect.

Adult flies expressing both Nep1KK101227 and ApplAβ.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4c739 (combined with tub-Gal80[ts] to limit expression to adulthood) do not show any defects in middle-term memory (MTM) tested in an aversive olfactory memory assay 2hr post-training, whereas with the Scer\GAL4VT064246 driver, the MTM is significantly reduced.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ApplAβ.Scer\UAS.T:Ivir\HA1
ApplAβ.UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (3)