sqhRLC.GFP(S65T) protein is associated with the basolateral cortex of neuroepithelial cells in the larval optic anlage, where it interacts with GFP-tagged asp protein.
In ex vivo third instar larval eye-antennal discs expressing sqhRLC.T:Avic\GFP-S65T, chromophore-assisted laser inactivation (CALI) of sqhRLC.T:Avic\GFP-S65T leads to a significant reduction in the extent of epithelial folding at the A1 fold, and disruption of the field boundary of the A1 fold, allowing cells to cross the boundary, in contrast to controls.
sqhRLC.T:Avic\GFP-S65T/sqhRLC.T:Avic\GFP-S65T does not affect egg length.
sqhRLC.GFP(S65T)/sqhRLC.GFP(S65T) is an enhancer of egg | germline clone phenotype of Pkn1T
sqhRLC.GFP(S65T)/sqhRLC.GFP(S65T) is an enhancer of egg | germline clone phenotype of Pkn2T
sqhRLC.T:Avic\GFP-S65T/sqhRLC.T:Avic\GFP-S65T enhances the shorter egg length phenotype seen in Pkn1T/Pkn1T or Pkn2T/Pkn2T germline clones.
sqhAX3 embryos which carry sqhRLC.T:Avic\GFP-S65T and which also express slmbScer\UAS.T:Zzzz\vhhGFP4 under the control of Scer\GAL4332.3 in the postmitotic amnioserosa cells show a dorsal open phenotype.
Expression of sqhRLC.T:Avic\GFP-S65T rescues the loss of rotation in the proventriculus and anterior midgut seen in sqhAX3/Y.
Carried in plasmid "RLC-GFP" and transfected into S2 cells.