FB2021_01, released Feb 18, 2021

Allele: Dmel\sqhRLC.GFP(S65T)

Open Close
General Information
Symbol
Dmel\sqhRLC.GFP(S65T)
Species
D. melanogaster
Name
FlyBase ID
FBal0221190
Feature type
allele
Associated gene
Dmel\sqh
Associated Insertion(s)
Carried in Construct
P{sqh-GFP.RLC}
Also Known As
Sqh-GFP, Sqh::GFP, RLC-GFP, P{sqh-GFP}, MRLC-GFP, sqh-sqh:GFP, sqhGFP, MyoII-GFP, Myosin-GFP, GFP-Sqh
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - coding region fusion
(Royou et al., 2004, Sisson et al., 2000)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{sqh-GFP.RLC}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The sqh coding region, which is tagged at the C-terminal end with a GFP(S65T) tag, is expressed under the control of the natural sqh promoter.

      (Royou et al., 2004)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      sqh
      Encoded product / tool
      sqh
      Tagged with
      GFP(S65T)
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult dorsal vessel
      (Alayari et al., 2009)
      third instar larval stage
      optic lobe neuroepithelial cell of larval optic anlage
      (Rujano et al., 2013)
      Additional Information
      Statement
      Reference

      sqhRLC.GFP(S65T) protein is associated with the basolateral cortex of neuroepithelial cells in the larval optic anlage, where it interacts with GFP-tagged asp protein.

      (Rujano et al., 2013)
       
      Marker for
      Reflects expression of
      sqh
      (Rujano et al., 2013, Alayari et al., 2009)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In ex vivo third instar larval eye-antennal discs expressing sqhRLC.T:Avic\GFP-S65T, chromophore-assisted laser inactivation (CALI) of sqhRLC.T:Avic\GFP-S65T leads to a significant reduction in the extent of epithelial folding at the A1 fold, and disruption of the field boundary of the A1 fold, allowing cells to cross the boundary, in contrast to controls.

      (Ku and Sun, 2017)

      sqhRLC.T:Avic\GFP-S65T/sqhRLC.T:Avic\GFP-S65T does not affect egg length.

      (Ferreira et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      sqhRLC.T:Avic\GFP-S65T/sqhRLC.T:Avic\GFP-S65T enhances the shorter egg length phenotype seen in Pkn1T/Pkn1T or Pkn2T/Pkn2T germline clones.

      (Ferreira et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference

      sqhAX3 embryos which carry sqhRLC.T:Avic\GFP-S65T and which also express slmbScer\UAS.T:Zzzz\vhhGFP4 under the control of Scer\GAL4332.3 in the postmitotic amnioserosa cells show a dorsal open phenotype.

      (Caussinus et al., 2011)
      Complementation and Rescue Data
      Rescues

      sqhRLC.GFP(S65T)/sqhRLC.T:Avic\GFP-S65T rescues sqhAX3

      (Okumura et al., 2010)

      sqhRLC.GFP(S65T)/sqhRLC.T:Avic\GFP-S65T rescues sqhAX3

      (Royou et al., 2004)
      Comments

      Expression of sqhRLC.T:Avic\GFP-S65T rescues the loss of rotation in the proventriculus and anterior midgut seen in sqhAX3/Y.

      (Okumura et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "RLC-GFP" and transfected into S2 cells.

      (Rogers et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Sqh-GFP
      (Hickson et al., 2006)
      sqhRLC.GFP(S65T)
      sqhRLC.T:Avic\GFP-S65T
      (Pulido Companys et al., 2020)
      sqhT:Avic\GFP
      (Bischoff and Cseresnyés, 2009)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0119137
      • FBal0177671
      References (106)