FlyBase Allele Report: Dmel\Myc[WT.UAS.Tag:HA]

General Information

Symbol

Dmel\Myc^{WT.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0221191

Feature type

allele

Associated gene

Dmel\Myc

Associated Insertion(s)

Carried in Construct

M{UAS-Myc.HA.WT}

Key Links

Transgenic product class

cDNA

(Steiger et al., 2008)

wild_type

(Steiger et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Steiger et al., 2008)

wild_type

(Steiger et al., 2008)

Mutagen

in vitro construct

(Schwinkendorf and Gallant, 2009, Steiger et al., 2008)

Progenitor genotype

Carried in construct

M{UAS-Myc.HA.WT}

Cytology

Description

UASt regulatory sequences drive expression of a full-length Myc cDNA tagged at the N-terminal end with Tag:HA.

(Steiger et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Myc

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^ap-md544

(Schwinkendorf and Gallant, 2009)

visible, with Scer\GAL4^ap-md544

(Schwinkendorf and Gallant, 2009)

Phenotype Manifest In

ommatidium, with Scer\GAL4^GMR.PB

(Schwinkendorf and Gallant, 2009)

ommatidium | somatic clone, with Scer\GAL4^αTub84B.PBb

(Schwinkendorf and Gallant, 2009)

wing, with Scer\GAL4^ap-md544

(Schwinkendorf and Gallant, 2009)

wing disc, with Scer\GAL4^ap-md544

(Schwinkendorf and Gallant, 2009)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Schwinkendorf and Gallant, 2009)

Detailed Description

Statement

Reference

Flies expressing dm^{WT.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PB show a significant increase in average ommatidial area compared to controls.

Flies expressing dm^{WT.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ap-md544 show a strong bending down of the wings.

Expression of dm^{WT.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ap-md544 induces apoptosis in the larval wing disc.

The average clonal area of clones in the larval wing disc expressing dm^{WT.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP is increased compared to that of control clones.

Expression of dm^{WT.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PBb in eyes using the "ey-FLP" system results in an increase in ommatidial size compared to controls, both when the eyes are dm[+] and when they are mutant for dm⁴.

(Schwinkendorf and Gallant, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Myc^{WT.UAS.Tag:HA}, Scer\GAL4^hh.PU is a non-suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Myc^{WT.UAS.Tag:HA}, Scer\GAL4^hh.PU is a non-suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^αTub84B.PBb/Myc^{WT.UAS.Tag:HA} partially rescues Myc⁴

(Steiger et al., 2008)

Fails to rescue

Scer\GAL4^Myc-PG45/Myc^{WT.UAS.Tag:HA} fails to rescue Myc^PG45

(Schwinkendorf and Gallant, 2009)

Comments

The expression of dm^{WT.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PBb in the eye (using the ey-FLP method) can rescue the reduced size but not the reduced number of ommatidia of eyes consisting of dm⁴ homozygous cells (induced using the ey-FLP method).

(Steiger et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64759

y¹ w^*; M{UAS-Myc.HA.WT}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Myc^{WT.Scer\UAS.T:Ivir\HA1}

Myc^{WT.UAS.Tag:HA}

dm^{WT.Scer\UAS.T:Ivir\HA1}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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