Gitex21C mutant adults exhibit severely reduced brain size as compared to controls, and a subset exhibit defects in mushroom body structure, with terminated alpha or beta lobes, or divided alpha lobes.
100% of homozygous escapers have defective wing morphology; the wings are crumpled, uneven and sometimes curled upwards or not fully spread. Gitex21C/Gitex51C adults also have a defective wing phenotype.
Homozygous escapers produce fewer fertilised eggs than normal.
Embryos lacking both maternal and zygotic Git[+] function show guidance defects in the VO5 and VO6 muscles, which are characterised by bypass and mistargeting of the mutant muscles towards the ventral midline; VO5 and VO6 muscles from opposite sides of late stage mutant embryos inappropriately attach at the ventral midline or fuse together at their meeting point, instead of terminating at a distance from the midline as occurs in wild-type embryos. 60% of VO5/VO6 muscles are affected to various degrees. The VA3 muscle occasionally shows mistargeting towards the ventral midline. There is a low level of increase in the number of lateral transverse (18% of hemisegments and VA3 (7.5%) muscles in these embryos, and in addition, there are low frequencies of muscle shape or attachment defects in the VO1 and LO1 muscles.
Embryos lacking zygotic Git[+] function show a moderate increase in muscle numbers compared to controls for LT (7.5%) and VA3 (3.7%) muscles and they also show defects in VA3 shape and targeting.