Allele Dmel\egg^{dsRNA.hp.Scer\UAS}

General Information
Symbol	Dmel\egg^{dsRNA.hp.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0221222
Feature type	allele	Associated gene	Dmel\egg

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Brower-Toland et al., 2009) in vitro construct - RNAi (Brower-Toland et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a hairpin repeat of sequences corresponding to part of egg. (Brower-Toland et al., 2009)
Carried in construct	P{UAS-dSETDB1.hp} (Brower-Toland et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| partially, with Scer\GAL4^Act5C.PU (Brower-Toland et al., 2009) locomotor behavior defective \| larval stage, with Scer\GAL4^Act5C.PU (Brower-Toland et al., 2009) short lived, with Scer\GAL4^Act5C.PU (Brower-Toland et al., 2009)
Phenotype Manifest In
	egg, with Scer\GAL4^nos.PG (Brower-Toland et al., 2009) wing, with Scer\GAL4^Act5C.PU (Brower-Toland et al., 2009)
Detailed Description
	Statement Reference Expression of egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[Act5C] results in a number of defects; larvae are more sluggish in their feeding and wandering behaviour than normal, animals often fail to eclose from the pupal case, and newly eclosed adults often fail to complete wing expansion. Surviving adult males and females have shorter lifespans than normal, often dying within 2 days of eclosion. Males show a significantly lower survival to adulthood than females. Expression of egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[nos.PG] decreased egg production. Expression of egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[da.G32] results in suppression of w variegation of the "118E-28" line (which contains the P{hsp26-pt-T}118E-28a and P{hsp26-pt-T}118E-28b insertions). Expression of egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[da.G32] results in suppression of w variegation of the P{hsp26-pt-T}39C-12, P{hsp26-pt-T}39C-2, P{hsp26-pt-T}118E-10 and P{hsp26-pt-T}118E-25-5 insertions. Expression of egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[nos.PG] has no effect on the degree of w variegation of the P{hsp26-pt-T}118E-10 and P{hsp26-pt-T}39C-12 insertions. Expression of egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[ey.200.Exel] results in suppression of w variegation of the P{hsp26-pt-T}118E-10 and P{hsp26-pt-T}39C-12 insertions. (Brower-Toland et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^Act5C.PU, egg^{dsRNA.hp.Scer\UAS} has lethal phenotype, enhanceable by G9a^{dsRNA.hp.Scer\UAS}, Scer\GAL4^Act5C.PU (Brower-Toland et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^Act5C.PU, egg^{dsRNA.hp.Scer\UAS} has lethal phenotype, suppressible by Scer\GAL4^Act5C.PU (Brower-Toland et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The degree of lethality of females expressing egg[dsRNA.hp.Scer\UAS] under the control of Scer\GAL4[Act5C] is decreased if they are also carrying Su(var)3-9[6]. (Brower-Toland et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dSETDB1^KD (Brower-Toland et al., 2009) dSETDB1hp^2101B (Brower-Toland et al., 2009) egg^{dsRNA.hp.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Brower-Toland et al., 2009, Genetics 181(4): 1303--1319 Multiple SET methyltransferases are required to maintain normal heterochromatin domains in the genome of Drosophila melanogaster. [FBrf0207712]

Allele Dmel\eggdsRNA.hp.Scer\UAS

Allele Dmel\egg^{dsRNA.hp.Scer\UAS}