FlyBase Allele Report: Dmel\moi[1]

General Information

Symbol

Dmel\moi¹

Species

D. melanogaster

Name

FlyBase ID

FBal0221227

Feature type

allele

Associated gene

Dmel\moi

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Raffa et al., 2009)

Mutagen

ethyl methanesulfonate

(Raffa et al., 2009)

Progenitor genotype

Cytology

Description

The point mutation within the moi coding sequence (the upstream open reading frame (ORF) of a dicistronic transcript that encodes both moi and Tgs1) also affects Tgs1 (the downstream ORF of the dicistronic transcript); in the presence of a transgene encoding moi[+], homozygotes for the mutation show substantially reduced levels of Tps1 protein compared to heterozygotes. FlyBase curator comment: the moi¹ allele represents the effect of the point mutation on the moi ORF, while the Tgs1^m1 allele represents the effect on Tgs1.

(Maccallini et al., 2020)

Amino acid replacement: G45E.

(Raffa et al., 2009)

Nucleotide substitution: G?A.

(Raffa et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:18,255,749..18,255,749 [+]

Nucleotide change:

G18255749A

Reported nucleotide change:

G?A

Amino acid change:

G45E | moi-PA

Reported amino acid change:

G45E

(Raffa et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with moi^CB02140)

(Raffa et al., 2009)

lethal (with moi^S096713)

(Raffa et al., 2009)

Phenotype Manifest In

chromosome, telomeric region

(Raffa et al., 2009)

chromosome, telomeric region (with Df(3R)P14)

(Raffa et al., 2009)

chromosome, telomeric region (with moi^CB02140)

(Raffa et al., 2009)

chromosome, telomeric region (with moi^S096713)

(Raffa et al., 2009)

Detailed Description

Statement

Reference

Mitotic cells from the brains of homozygous larvae have frequent telomere fusions, with an average of 5.6 telomere associations per cell. Double telomere associations (DTAs), in which 2 sister telomeres fuse with another pair of sister telomeres are approximately 4-fold more frequent that single telomere associations (STAs), in which a single telomere associates with either its sister or a nonsister telomere.

Mitotic cells from the brains of moi¹/Df(3R)P14 larvae show frequent telomere fusions, with DTAs being more common than STAs.

Mitotic cells from the brains of moi¹/moi^S096713 and moi¹/moi^CB02140 larvae show frequent telomere fusions.

(Raffa et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

moi¹/moi[+] is a suppressor | partially of male semi-sterile phenotype of cav¹/Df(3R)Exel6198, cav^HipHop.GFP

(Dubruille and Loppin, 2015)

Other

Statement

Reference

moi¹/moi[+], peo¹ has abnormal mitotic cell cycle | larval stage phenotype

(Cenci et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

moi¹/moi[+], peo¹ has larval neuroblast phenotype

(Cenci et al., 2015)

moi¹/moi[+], peo¹ has chromosome | larval stage phenotype

(Cenci et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

peo¹, moi¹ double heterozygosity leads to telomeric fusions in larval neuroblasts.

(Cenci et al., 2015)

The slightly reduced fertility of cav^{HipHop.T:Avic\GFP}/Y;cav¹/Df(3R)Exel6198 adult (cav¹ rescue) males is moderately improved by combination with moi¹.

The increased number of abnormal spermatid nuclei (showing chromatin bridges) characteristic for adult cav^{HipHop.T:Avic\GFP};cav¹/Df(3R)Exel6198 males is strongly enhanced by combination with Df(3R)ms(3)K81-2 in homozygous or, to a lesser degree, in heterozygous state. Enhancement of the abnormal nuclear phenotype is also observed upon combination with single copy of HipHop¹ as well as ver^S147910 but the phenotype is not aggravated further by combination with heterozygous moi¹ allele.

(Dubruille and Loppin, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

moi^S096713

(Raffa et al., 2009)

moi^CB02140

(Raffa et al., 2009)

Rescued by

moi¹/Df(3R)P14 is rescued by moi^R1.Tub

(Raffa et al., 2009)

moi¹/Df(3R)P14 is rescued by moi^R2.Tub

(Raffa et al., 2009)

moi¹/Df(3R)P14 is rescued by moi^R3.Tub

(Raffa et al., 2009)

moi¹/moi^S096713 is rescued by moi^R1.Tub

(Raffa et al., 2009)

moi¹/moi^S096713 is rescued by moi^R2.Tub

(Raffa et al., 2009)

moi¹/moi^S096713 is rescued by moi^R3.Tub

(Raffa et al., 2009)

moi^CB02140/moi¹ is rescued by moi^R1.Tub

(Raffa et al., 2009)

moi^CB02140/moi¹ is rescued by moi^R2.Tub

(Raffa et al., 2009)

moi^CB02140/moi¹ is rescued by moi^R3.Tub

(Raffa et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: a second site lethal mutation on the chromosome.

(Raffa et al., 2009)

The mutation in the moi¹ mutant is in the upstream ORF (moi) of a dicistronic locus that encodes moi (upstream ORF) and tatl (downstream ORF). The telomere fusion phenotype of moi¹ homozygotes and the telomere fusion and lethal phenotypes of moi¹/Df(3R)P14 animals are fully rescued by a transgene containing only the moi ORF, indicating that the lesion appears to affect only moi and not tatl.

(Raffa et al., 2009)

Comments

Allelic series (with respect to severity of telomere fusion phenotype): moi¹ > moi^CB02140 = moi^M12 > moi^S096713.

(Raffa et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

moi¹

(Maccallini et al., 2020, Cicconi et al., 2017, Cenci et al., 2015, Dubruille and Loppin, 2015, Raffa et al., 2010, Raffa et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close