Allele Dmel\ATP7^{Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\ATP7^{Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0221296
Feature type	allele	Associated gene	Dmel\ATP7

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Norgate et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Expression of ATP7 cDNA (amplified from S2 cell cDNA with primers: F1 5' gaGGTACCatgtccacggtgcgcctgcc 3' and R1 5' gcTCTAGAcagcttttgcagttcggtAct 3'), fused in frame at the c-terminus to the epitope flag T:Zzzz\FLAG, is under the control of Scer\UAS sequences. (Norgate et al., 2006)
Carried in construct	P{UAS-ATP7.FLAG} (Norgate et al., 2006, Southon et al., 2008, Binks et al., 2010)
Tagged with	T:Zzzz\FLAG (Norgate et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^Act5C.PI (Norgate et al., 2006) body color defective, with Scer\GAL4^pnr-MD237 (Norgate et al., 2006, Southon et al., 2008) body color defective \| adult stage, with Scer\GAL4^pnr-MD237 (Binks et al., 2010) lethal \| pupal stage, with Scer\GAL4^tracheal (Norgate et al., 2006) partially lethal - majority die \| third instar larval stage, with Scer\GAL4^tracheal (Norgate et al., 2006)
Phenotype Manifest In
	adult cuticle, with Scer\GAL4^pnr-MD237 (Binks et al., 2010) adult thoracic sensillum, with Scer\GAL4^pnr-MD237 (Southon et al., 2008) adult thorax, with Scer\GAL4^pnr-MD237 (Southon et al., 2008) macrochaeta & mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Norgate et al., 2006) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Norgate et al., 2006) scutellar bristle, with Scer\GAL4^ptc-559.1 (Norgate et al., 2006) scutellum, with Scer\GAL4^pnr-MD237 (Norgate et al., 2006) scutellum, with Scer\GAL4^ptc-559.1 (Norgate et al., 2006) tergite, with Scer\GAL4^Act5C.PI (Norgate et al., 2006) trident, with Scer\GAL4^pnr-MD237 (Norgate et al., 2006)
Detailed Description
	Statement Reference Expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237] results in a hypopigmentation phenotype in the adult dorsal cuticle of the thorax and abdomen (in the domain of Scer\GAL4 expression). Expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] has no effect on the eye. (Binks et al., 2010) Expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237] causes hypopigmentation and loss of sensory bristles in the pnr region, and reduced thoracic width in adult flies. (Southon et al., 2008) ATP7[Scer\UAS.T:Zzzz\FLAG]; Scer\GAL4[Act5C.PI] grown on copper deficient medium have reduced abdominal pigmentation. This phenotype is largely rescued by adding back copper to the medium, but not by supplementing the copper deficient medium with iron or zinc. ATP7[Scer\UAS.T:Zzzz\FLAG]; Scer\GAL4[pnr-MD237] adults have a non pigmented stripe running down the midline of the notum. They also have a cleft in the thorax, reduction in the scutellum and variable loss of thoracic bristles. This loss of pigmentation is independent of dietary copper levels. ATP7[Scer\UAS.T:Zzzz\FLAG]; Scer\GAL4[tracheal] animals die as late larvae or early pupae. ATP7[Scer\UAS.T:Zzzz\FLAG]; Scer\GAL4[ptc-559.1] adults show reduction of the scutellum and partial or complete loss of scutellar bristles. (Norgate et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective phenotype, enhanceable by Mvl^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Southon et al., 2008)
NOT Enhanced by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective phenotype, non-enhanceable by Mvl^97f/Mvl[+] (Southon et al., 2008)
Suppressed by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective \| adult stage phenotype, suppressible by Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Binks et al., 2010) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective \| adult stage phenotype, suppressible by Ctr1B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Binks et al., 2010) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective phenotype, suppressible by Ctr1A^{Scer\UAS.T:Zzzz\FLAG} (Norgate et al., 2006)
NOT suppressed by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective \| adult stage phenotype, non-suppressible by Scox^GD898, Scer\GAL4^pnr-MD237 (Binks et al., 2010) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective phenotype, non-suppressible by Atox1^{Scer\UAS.T:Zzzz\FLAG} (Norgate et al., 2006) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective phenotype, non-suppressible by Ccs^{Scer\UAS.T:Zzzz\FLAG} (Norgate et al., 2006) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has body color defective phenotype, non-suppressible by Mvl^97f/Mvl[+] (Southon et al., 2008)
Suppressor of
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa is a suppressor of visible phenotype of Ctr1B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa (Binks et al., 2010)
NOT Suppressor of
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa is a non-suppressor of visible phenotype of Ctr1A^GD16726, Scer\GAL4^GMR.PFa (Binks et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, enhanceable by Mvl^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Southon et al., 2008)
NOT Enhanced by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-enhanceable by Mvl^97f/Mvl[+] (Southon et al., 2008)
Suppressed by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, suppressible by Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Binks et al., 2010) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, suppressible by Ctr1B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Binks et al., 2010) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has trident phenotype, suppressible by Ctr1A^{Scer\UAS.T:Zzzz\FLAG} (Norgate et al., 2006)
NOT suppressed by
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has adult cuticle phenotype, non-suppressible by Scox^GD898, Scer\GAL4^pnr-MD237 (Binks et al., 2010) ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by Mvl^97f/Mvl[+] (Southon et al., 2008)
Suppressor of
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Ctr1B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa (Binks et al., 2010)
NOT Suppressor of
Statement Reference ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Ctr1A^GD16726, Scer\GAL4^GMR.PFa (Binks et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of either Ctr1A[Scer\UAS.T:Zzzz\FLAG] or Ctr1B[Scer\UAS.T:Zzzz\FLAG] rescues the cuticle hypopigmentation phenotype caused by expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237]. Co-expression of Scox[GD898] does not suppress the cuticle hypopigmentation phenotype caused by expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237]. Co-expression of ATP7[Scer\UAS.T:Zzzz\FLAG] suppresses the rough eye phenotype caused by expression of Ctr1B[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa]. Co-expression of ATP7[Scer\UAS.T:Zzzz\FLAG] has no effect on the eye phenotype caused by expression of Ctr1A[GD16726] under the control of Scer\GAL4[GMR.PFa]. (Binks et al., 2010) Expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237] in a Mvl[97f]/+ background does not significantly affect pigmentation or thoracic development compared to overexpression in a wildtype background. Simultaneous overexpression of ATP7[Scer\UAS.T:Zzzz\FLAG] and Mvl[Scer\UAS.T:Zzzz\FLAG] by Scer\GAL4[pnr-MD237] causes severe hypopigmentation and a further reduction in thorax width compared to overexpression of ATP7[Scer\UAS.T:Zzzz\FLAG] alone. (Southon et al., 2008) The loss of pigmentation seen in a stripe down the midline of the notum of ATP7[Scer\UAS.T:Zzzz\FLAG]; Scer\GAL4[pnr-MD237] adults is suppressed by Ctr1A[Scer\UAS.T:Zzzz\FLAG] but not by CCS[Scer\UAS.T:Zzzz\FLAG] or Atox1[Scer\UAS.T:Zzzz\FLAG]. (Norgate et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, ATP7^{Scer\UAS.T:Zzzz\FLAG} rescues ATP7^ΔP17 (Norgate et al., 2006) Scer\GAL4^pnr-MD237/ATP7^{Scer\UAS.T:Zzzz\FLAG} rescues ATP7^GD3322 (Binks et al., 2010)
Comments	Co-expression of ATP7[Scer\UAS.T:Zzzz\FLAG] rescues the cuticle hypopigmentation phenotype caused by expression of ATP7[GD3322] under the control of Scer\GAL4[pnr-MD237]. (Binks et al., 2010) Lethality due to ATP7[ΔP17]/Y is rescued by ATP7[Scer\UAS.T:Zzzz\FLAG]; Scer\GAL4[Act5C.PI]. (Norgate et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ATP7^{Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Binks et al., 2010, J. Biol. Inorg. Chem. 15(4): 621--628 Tissue-specific interplay between copper uptake and efflux in Drosophila. [FBrf0210589] Southon et al., 2008, J. Exp. Biol. 211(5): 709--716 Malvolio is a copper transporter in Drosophila melanogaster. [FBrf0203166] Norgate et al., 2006, Mol. Biol. Cell 17(1): 475--484 Essential roles in development and pigmentation for the Drosophila copper transporter DmATP7. [FBrf0191161]

Allele Dmel\ATP7Scer\UAS.T:Zzzz\FLAG

Allele Dmel\ATP7^{Scer\UAS.T:Zzzz\FLAG}