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General Information
Symbol
Dmel\ATP7UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0221296
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ATP7, UAS-ATP7FLAG
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of ATP7 cDNA (amplified from S2 cell cDNA with primers: F1 5' gaGGTACCatgtccacggtgcgcctgcc 3' and R1 5' gcTCTAGAcagcttttgcagttcggtAct 3'), fused in frame at the c-terminus to the epitope flag Tag:FLAG, is under the control of UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ATP7Scer\UAS.T:Zzzz\FLAG driven in the thoracic midline by Scer\GAL4pnr-MD237 causes a mild thoracic cleft and hypo-pigmentation of the thorax and abdomen, compared to controls.

Expression of ATP7Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 does not significantly affect larval lethality compared to controls. Scer\GAL4CCAP.PP>ATP7Scer\UAS.T:Zzzz\FLAG third instar larval brains do not show a significant decrease in the density of axon branching or number of cell bodies or area of neurons compared to controls.

Expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 results in reduced survival from first instar larval stage to adulthood, as compared with controls, with most fatalities occurring at the pharate adult stage, and a subset of adults display an unexpanded wing phenotype and tanning defects; but does not lead to any delay in developmental time. Lethality and the unexpanded wing phenotype are both enhanced as compared to controls upon dietary supplementation with the copper chelator bathocuproine disulfonate at 300uM. The expanded wing phenotype is not significantly ameliorated by the addition of a Scer\GAL80CCAP.PL construct to inhibit expression in the CCAP cells.

Expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PP does not cause any significant increase in the number of adults with unexpanded wings, as compared to controls.

Animals expressing ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4mex1.2.1 are sensitive to dietary copper compared to controls.

Expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237 results in a hypopigmentation phenotype in the adult dorsal cuticle of the thorax and abdomen (in the domain of Scer\GAL4 expression).

Expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa has no effect on the eye.

Expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237 causes hypopigmentation and loss of sensory bristles in the pnr region, and reduced thoracic width in adult flies.

ATP7Scer\UAS.T:Zzzz\FLAG; Scer\GAL4Act5C.PI grown on copper deficient medium have reduced abdominal pigmentation. This phenotype is largely rescued by adding back copper to the medium, but not by supplementing the copper deficient medium with iron or zinc. ATP7Scer\UAS.T:Zzzz\FLAG; Scer\GAL4pnr-MD237 adults have a non pigmented stripe running down the midline of the notum. They also have a cleft in the thorax, reduction in the scutellum and variable loss of thoracic bristles. This loss of pigmentation is independent of dietary copper levels.

ATP7Scer\UAS.T:Zzzz\FLAG; Scer\GAL4tracheal animals die as late larvae or early pupae.

ATP7Scer\UAS.T:Zzzz\FLAG; Scer\GAL4ptc-559.1 adults show reduction of the scutellum and partial or complete loss of scutellar bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of ATP7Scer\UAS.T:Zzzz\FLAG enhances the thoracic cleft and scutellum loss seen in flies with expression of Grx1GD10587 driven by Scer\GAL4pnr-MD237 (compared to expression of either alone).

Co-expression of ATP7Scer\UAS.T:Zzzz\FLAG significantly enhances larval lethality seen with expression of GclcGD9767 driven by Scer\GAL4elav-C155. Co-expression of ATP7Scer\UAS.T:Zzzz\FLAG significantly exacerbates the decrease of axon branching and Scer\GAL4CCAP.PP neuron area in third instar larval brains with expression of GclcGD9767 driven by Scer\GAL4CCAP.PP.

Co-expression of Ctr1AGD16726 and ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PP causes a highly penetrant unexpanded wing phenotype, as compared to controls.

Co-expression of PsnKK109329 suppresses the copper sensitivity seen in animals expressing ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4mex1.2.1.

Co-expression of either Ctr1AScer\UAS.T:Zzzz\FLAG or Ctr1BScer\UAS.T:Zzzz\FLAG rescues the cuticle hypopigmentation phenotype caused by expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237.

Co-expression of ScoxGD898 does not suppress the cuticle hypopigmentation phenotype caused by expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237.

Co-expression of ATP7Scer\UAS.T:Zzzz\FLAG suppresses the rough eye phenotype caused by expression of Ctr1BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa.

Co-expression of ATP7Scer\UAS.T:Zzzz\FLAG has no effect on the eye phenotype caused by expression of Ctr1AGD16726 under the control of Scer\GAL4GMR.PFa.

Expression of ATP7Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237 in a Mvl[97f]/+ background does not significantly affect pigmentation or thoracic development compared to overexpression in a wildtype background.

Simultaneous overexpression of ATP7Scer\UAS.T:Zzzz\FLAG and MvlScer\UAS.T:Zzzz\FLAG by Scer\GAL4pnr-MD237 causes severe hypopigmentation and a further reduction in thorax width compared to overexpression of ATP7Scer\UAS.T:Zzzz\FLAG alone.

The loss of pigmentation seen in a stripe down the midline of the notum of ATP7Scer\UAS.T:Zzzz\FLAG; Scer\GAL4pnr-MD237 adults is suppressed by Ctr1AScer\UAS.T:Zzzz\FLAG but not by CCSScer\UAS.T:Zzzz\FLAG or Atox1Scer\UAS.T:Zzzz\FLAG.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expression of ATP7Scer\UAS.T:Zzzz\FLAG rescues the cuticle hypopigmentation phenotype caused by expression of ATP7GD3322 under the control of Scer\GAL4pnr-MD237.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ATP7Scer\UAS.T:Zzzz\FLAG
ATP7UAS.Tag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (8)