Allele Dmel\Ctr1A^{Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\Ctr1A^{Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0221300
Feature type	allele	Associated gene	Dmel\Ctr1A

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Norgate et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an N-terminally truncated version of Ctr1A, which starts at residue M22. (Binks et al., 2010) Expression of Ctr1A cDNA (amplified from S2 cell cDNA with primers: F1 5' gaGGTACCatgcaccacgatcacagcg 3' and R1 5'gcTCTAGAgtgacagtgctcggttacg 3'), fused in frame at the c-terminus to the epitope flag T:Zzzz\FLAG, is under the control of Scer\UAS sequences. (Norgate et al., 2006)
Carried in construct	P{UAS-Ctr1A.FLAG} (Norgate et al., 2006, Binks et al., 2010)
Tagged with	T:Zzzz\FLAG (Norgate et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa] has no effect on the eye. (Binks et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Ctr1B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa (Binks et al., 2010)
Suppressor of
Statement Reference Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 is a suppressor of body color defective \| adult stage phenotype of ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Binks et al., 2010) Ctr1A^{Scer\UAS.T:Zzzz\FLAG} is a suppressor of body color defective phenotype of ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Norgate et al., 2006)
NOT Suppressor of
Statement Reference Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 is a non-suppressor of visible phenotype of Scer\GAL4^pnr-MD237, Scox^GD898 (Binks et al., 2010)
Other
Statement Reference ATP7^GD3322, Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa has eye color defective \| female phenotype (Binks et al., 2010) ATP7^GD3322, Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa has lethal \| male phenotype (Binks et al., 2010) ATP7^GD3322, Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa has visible \| female phenotype (Binks et al., 2010)
Phenotype Manifest In
Enhancer of
Statement Reference Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Ctr1B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa (Binks et al., 2010)
Suppressor of
Statement Reference Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 is a suppressor of adult cuticle phenotype of ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Binks et al., 2010) Ctr1A^{Scer\UAS.T:Zzzz\FLAG} is a suppressor of trident phenotype of ATP7^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Norgate et al., 2006)
NOT Suppressor of
Statement Reference Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of Scer\GAL4^pnr-MD237, Scox^GD898 (Binks et al., 2010) Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 is a non-suppressor of chaeta phenotype of Scer\GAL4^pnr-MD237, Scox^GD898 (Binks et al., 2010) Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 is a non-suppressor of scutellum phenotype of Scer\GAL4^pnr-MD237, Scox^GD898 (Binks et al., 2010)
Other
Statement Reference ATP7^GD3322, Ctr1A^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^GMR.PFa has eye \| female phenotype (Binks et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] rescues the cuticle hypopigmentation phenotype caused by expression of ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237]. Co-expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] has no effect on the "Minute"-like phenotype caused by expression of Scox[GD898] under the control of Scer\GAL4[pnr-MD237]. Co-expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] enhances the rough eye phenotype caused by expression of Ctr1B[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PFa]. Co-expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] and ATP7[GD3322] under the control of Scer\GAL4[GMR.PFa] results in male lethality, while females have a severe rough eye phenotype. (Binks et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Ctr1A^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^pnr-MD237 rescues Ctr1A^GD16726 (Binks et al., 2010)
Fails to rescue	Ctr1A^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^GMR.PFa fails to rescue Ctr1A^GD16726 (Binks et al., 2010)
Comments	Co-expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] rescues the cuticle hypopigmentation phenotype caused by expression of a weak Ctr1A[GD16726] line under the control of Scer\GAL4[pnr-MD237]. Co-expression of Ctr1A[Scer\UAS.T:Zzzz\FLAG] does not rescue the eye phenotype caused by expression of Ctr1A[GD16726] under the control of Scer\GAL4[GMR.PFa]. (Binks et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Ctr1A^{Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Binks et al., 2010, J. Biol. Inorg. Chem. 15(4): 621--628 Tissue-specific interplay between copper uptake and efflux in Drosophila. [FBrf0210589] Norgate et al., 2006, Mol. Biol. Cell 17(1): 475--484 Essential roles in development and pigmentation for the Drosophila copper transporter DmATP7. [FBrf0191161]

Allele Dmel\Ctr1AScer\UAS.T:Zzzz\FLAG

Allele Dmel\Ctr1A^{Scer\UAS.T:Zzzz\FLAG}