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General Information
Symbol
Avic\GFPEGFP.ppk.1
Species
A. victoria
Name
FlyBase ID
FBal0221331
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ppk-EGFP, ppk-GFP, ppk-EGFP5, pickpocket-EGFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A ~1kb fragment from the ppk enhancer region fused to a minimal Hsp70 promoter drives expression of EGFP.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Avic\GFPEGFP.ppk.1 drives expression in 4 clusters of neurons in the adult female genital tract: one cluster of 2 neurons in the lateral oviduct (with one neuron co-localising with Scer\GAL4dsx.KI and the other with Ecol\lexAfru.P1.T:Hsim\VP16) and three clusters in the uterus. An anterior one with 3 neurons (which also express Scer\GAL4dsx.KI) and one more posterior with 7 neurons which co-express Scer\GAL4dsx.KI, with 3 neurons of those also expressing Ecol\lexAfru.P1.T:Hsim\VP16. The most posterior cluster in the uterus is composed of 5 neurons.

Signal is observed in the axonal terminal branches of of class IV dendritic arborizing (C4da) neurons in the ventral nerve cord as a ladder-like pattern with a longitudinal fascicle on each side and a commissural fascicle in every segment.

Avic\GFPEGFP.ppk.1 is expresssed in 2-3 Scer\GAL4fru.P1.D positive fruitless expressing neurons either side of the uterus.

In females, Avic\GFPEGFP.ppk.1 drives expression in 2 neurons on each lateral oviduct and 15 neurons on each side of the uterus, organised in 3 clusters. Eight of these reproductive tract neurons labeled by Avic\GFPEGFP.ppk.1 co-express Scer\GAL4fru.P1.D. In males, Avic\GFPEGFP.ppk.1 labels 7 neurons in the ejaculatory duct, with two of these co-expressing Scer\GAL4fru.P1.D.

This reporter reproduces the expression of the ppk gene in Type IV dendritic aborising neurons. There is also a low level of expression in some Type III neurons.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Avic\GFP1.E.ppk
Avic\GFP5.E.ppk
Avic\GFPEGFP.ppk.1
Name Synonyms
pick-pocket-EGFP
Secondary FlyBase IDs
  • FBal0148470
  • FBal0190823
References (26)