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General Information
Symbol
Dmel\Vps16AdsRNA.UAS.WIZ
Species
D. melanogaster
Name
FlyBase ID
FBal0221466
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat of an approximately 300bp fragment of Vps16A cDNA sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Vps16AdsRNA.Scer\UAS.WIZ in posterior epidermal cells under the control of Scer\GAL45-56 prevents phagocytic clearance of ddaC neuron dendrite debris following pupal stage dendritic pruning. Similarly, when larval dendrites are injured (severed with an infrared laser) expression of Vps16AdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4A58 blocks dendritic debris clearance.

Knock-down of Vps16A through expression of Vps16AdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32 results in elevated levels of bacteria in primary hemocytes, suggesting an inability to digest them.

Dextran phagocytosed in LysoTracker experiments fails to reach lysosomes in flies expressing Vps16AdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32 indicating lysosomal dysfunction rather than a defect in phagocytosis.

Expression of Vps16AdsRNA.Scer\UAS.WIZ driven by Scer\GAL4da.G32 leads to enlarged endosomes that are twice as big as those in wild-type.

Expression of Vps16AdsRNA.Scer\UAS.WIZ under the simultaneous control of both Scer\GAL4GMR.PF and Scer\GAL4ey.PH results in a dramatic reduction of eye pigmentation compared to wild type. There is a gradient of pigmentation with the most anterior ommatidia having lost most of their pigmentation. The lenses overlaying each ommatidium appear correctly formed, but the pseudocones are essentially absent, with the remnants that are present being surrounded by a dense structure that is composed of vesicles and vacuolar structures containing internal vesicles. Type I pigment granules (normally found in primary pigment cells) and type II pigment granules (normally found in secondary pigment cells) are absent. The pigment cells are full of vacuoles and autophagosomes resulting in the pigment cell layer being expanded in width. Most photoreceptor cells contain only remnants of rhabdomeres, and the loss of rhabdomeres is accompanied by the accumulation of autophagosomes and vacuoles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4da.G32, Vps16AdsRNA.UAS.WIZ has endosome phenotype, enhanceable by emaf07675/CG12753[+]

Additional Comments
Genetic Interactions
Statement
Reference

Knockdown of Vps16A through expression of Vps16AdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4Scer\FRT.Act5C moderately enhances the overgrowth seen in Ras85DV12.Scer\UAS, Scer\GAL4Scer\FRT.Act5C tumour cells and leads to tumour invasion towards the ventral nerve cord.

emaf07675/+ enhances the endosomal phenotype observed in cell expressing Vps16AdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32, as there is an additional twofold increase in the size of endosomes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Vps16AdsRNA.Scer\UAS.WIZ
Vps16AdsRNA.UAS.WIZ
Name Synonyms
Secondary FlyBase IDs
    References (5)